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Analysis of storage proteins (prolamines, puroindolines and Waxy) in common wheat lines Triticum aestivum L. × (Triticum timopheevii Zhuk. × Triticum tauschii) with complex resistance to fungal infections

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Abstract

Storage proteins (prolamines, puroindolines, and Waxy) were studied in common wheat introgression lines obtained with the use of the Saratovskaya 29 (S29) cultivar line and synthetic hexaploid wheat (Triticum timopheevii Zhuk. × T. tauschii) (Sintetik, Sin.) displaying complex resistance to fungal infections. Comparative analysis of storage proteins in the introgression lines of common wheat Triticum aestivum L. and in the parental forms revealed the only line (BC5) having a substitution at the Gli-B2 locus from Sintetik. Hybrid lines subjected to nine backcrosses with the recurrent parental form S29 and selections for resistance to pathogens can be considered as nearly isogenic for the selected trait and retaining the allelic composition of (1) prolamines responsible for the bread-making qualitiy, (2) puroindolines associated with grain texture, and (3) Waxy proteins responsible for nutritive qualities. These lines are valuable as donors of immunity in breeding programs without the loss of the quality of flour and grain as compared to the S29 line and are also important in searching for genes determining resistance to leaf and stem rust and to powdery mildew. The amphiploid has a number of characters (silent Glu-A1 locus and Ha genotype) that can negatively affect the quality of flour and grain and thus should be taken into account when choosing this donor.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    L. V. Obukhova, L. I. Laikova & V. K. Shumny

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  1. L. V. Obukhova
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  2. L. I. Laikova
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  3. V. K. Shumny
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Correspondence to L. V. Obukhova.

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Original Russian Text © L.V. Obukhova, L.I. Laikova, V.K. Shumny, 2010, published in Genetika, 2010, Vol. 46, No. 6, pp. 764–768.

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Obukhova, L.V., Laikova, L.I. & Shumny, V.K. Analysis of storage proteins (prolamines, puroindolines and Waxy) in common wheat lines Triticum aestivum L. × (Triticum timopheevii Zhuk. × Triticum tauschii) with complex resistance to fungal infections. Russ J Genet 46, 672–676 (2010). https://doi.org/10.1134/S1022795410060062

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