Abstract
Fluorescence of H3-p histone and DAPI was studi ed at different stages of interphase and mitosis in cells of imaginal disks of third-instar Drosophila melanogaster larvae. Three stages differing in the spatial organization of the chromosome set in mitosis were revealed. At the first stage (prophase, prometaphase), the histone 3 phosphorylation level rises, and the volume occupied by the chromosome set in the nucleus increases. The distinctive features of the second stage (metaphase) are a gradual decrease in the histone 3 phosphorylation (the density of phosphorylation remaining constant) and a reduction of the volume occupied by the chromosome set. At the third stage (anaphase, telophase), the intensity and density of the signal from H3-p histone decrease, and the volume occupied by the chromosome set reduces. At this stage, in Mer 4 larvae, in contrast to the control strain, the cells prematurely pass from anaphase into telophase. In addition, a subpopulation of cells with an abnormally large volume of nuclear DNA during the G1 period was revealed in Mer 4 larvae. The cells of this subpopulation do not enter into the DNA synthesis and quit the cycle.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wei, Y.Yu.L., Bowen, J., Gorovsky, M.A., and Allis, C.D., Phosphorylation of Histone H3 Is Required for Proper Chromosome Condensation and Segregation, Cell, 1999, vol. 97, pp. 99–109.
Hirano, T., Chromosome Cohesion, Condensation, and Separation, Annu. Rev. Biochem., 2000, vol. 69, pp. 115–144.
Hsu, J.Y., Sun, Z.W., Li, X., et al., Mitotic Phosphorilation of Histone H3 Is Governed by Ip 11/Aurora Kinase and g1c/PP1 Phosphotase in Budding Yeast and Nematods, Cell, 2000, vol. 102, pp. 279–291.
Speliotes, E.K., Uren, A., Vaux, D., and Horvitz, H.R., The Survivin-Like C. elegans DIR-1 Protein Acts with the Aurora-Like Kinase AIR-2 to Control Chromosome Behavior and Spindle Midzone Organization, Mol. Cell, 2000, vol. 6, pp. 211–223.
Omelyanchuk, L.V., Nokkala, S., Mattila, Ya., et al., The Distribution of Mitoses in the Imaginal Disks of Third-Instar Drosophila melanogaster Larvae, Russ. J. Genet., 2007, vol. 43, no. 7, pp. 769–775.
Lebedeva, L. I., Trunova, S.A., and Omelyanchuk, L.V., Genetics of the Cell Cycle: Adaptive Modification of the CycB2g Mutation Expression in Dividing Cells of Drosophila melanogaster, Russ. J. Genet., 2005, vol. 41, no. 3, pp. 237–243.
Tsuji, H. and Tobari, I., Effects of 5-Bromodeoxyuridine on Metamorphosis and on Cell Cycle in Drosophila melanogaster, Exp. Cell Res., 1980, vol. 125, pp. 79–85.
Milan, M., Compuzano, S., and Garcia-Bellido, A., Cell Cycling and Patterned Cell Proliferation in the Wing Primordium of Drosophila, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 640–615.
Latham, K.E., Mechanisms and Control of Embryonic Genome Activation in Mammalian Embryos, Int. Rev. Cytol., 1999, vol. 193, pp. 71–124.
Schmiesing, J.A., Gregson, H.C., Zhou, S., and Yoko-mori, K., A Human Condensing Complex Containing hCAP-C/hCAP-E and CNAP1, a Homolog of Xenopus XCAP-D2, Colocalizes with Phosphorylated Histone H3 during the Early Stage of Mitotic Chromosome Condensation, Mol. Cell. Biol., 2000, vol. 20, pp. 6996–7006
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © L.I. Lebedeva, E.M. Akhmametyeva, L. V. Omelyanchuk, 2010, published in Genetika, 2010, Vol. 46, No. 2, pp. 180–186.
Rights and permissions
About this article
Cite this article
Lebedeva, L.I., Akhmametyeva, E.M. & Omelyanchuk, L.V. Dynamics of the spatial organization of the chromosome set in cells of Drosophila melanogaster imaginal disks normally and under the action of the tumor-inducing mutation Merlin . Russ J Genet 46, 157–163 (2010). https://doi.org/10.1134/S1022795410020043
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795410020043