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Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene

  • Plant Genetics
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Abstract

The buckwheat serine protease inhibitor (BWI-1) target gene (ISP) was expressed under the control of the constitutive 35S promoter of the cauliflower mosaic virus was expressed in transgenic tobacco plants and conferred antibacterial resistance. A stable and linked inheritance and expression of the marker nptII and target genes were observed in a random sample of independent transgenic tobacco plants after longterm propagation by nodal segments or multiple (for 1.5 years) regenerations under nonselective conditions; the transgene insert was preserved in the T1 seed progeny. Transgenic plants displayed numerous alterations in microsporogenesis. A loss of kanamycin (Km) resistance was accompanied by a loss of antibacterial activity in two lines. Segregation was observed for Km resistance in line C7 and for seedling size in line C22.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    N. V. Khadeeva & E. Yu. Yakovleva

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  1. N. V. Khadeeva
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  2. E. Yu. Yakovleva
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Correspondence to N. V. Khadeeva.

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Original Russian Text © N.V. Khadeeva, E.Yu. Yakovleva, 2010, published in Genetika, 2010, Vol. 46, No. 1, pp. 58–65.

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Khadeeva, N.V., Yakovleva, E.Y. Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene. Russ J Genet 46, 50–56 (2010). https://doi.org/10.1134/S1022795410010084

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