Skip to main content
SpringerLink
Log in

Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants

  • Plant Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm’ region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity of A. vernalis populations were determined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. McClintok, B., Controlling Elements and the Gene, Cold Spring Harbor Symp. Quant. Biol., 1956, vol. 21, pp. 197–216.

    Google Scholar 

  2. Ilyin, Y.V., Tchurikov, N.A., Ananiev, E.V., et al., Studies on the DNA Fragments and Adjacent Sequences, Cold Spring Harbor Symp. Quant. Biol., 1978, vol. 42, pp. 959–969.

    PubMed  Google Scholar 

  3. Lyubomirskaya, N.V. and Il’in, Yu.V., Mobile Genetic Elements of Eukaryotes: Past, Present, Future, Mol. Biol. (Moscow), 1999, vol. 33, no. 6, pp. 958–968.

    Google Scholar 

  4. Kalendar, R., Tanskanen, J., Immonen, S., et al., Genome Evolution of Wild Barley (Hordeum spontaneum) by BARE-1 Retrotransposon Dynamics in Response to Sharp Microclimatic Divergence, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, no. 12, pp. 6603–6607.

    Article  CAS  PubMed  Google Scholar 

  5. Evgen’ev, M.B., Mobile Elements and Genome Evolution, Mol. Biol. (Moscow), 2007, vol. 41, no. 2, pp. 234–245.

    Google Scholar 

  6. Kumar, A. and Bennetzen, J., Plant Retrotransposons, Annu. Rev. Genet., 1999, vol. 33, pp. 479–532.

    Article  CAS  PubMed  Google Scholar 

  7. Wicker, T., Sabot, F., Hua-Van, A., et al., A Unified Classification System for Eukaryotic Transposable Elements, Nat. Rev. Genet., 2007, no. 8, pp. 973–982.

  8. Kalendar, R., Grob, T., Regina, M., et al., IRAP and REMAP: Two New Retrotransposon-Based DNA Fingerprinting Techniques, Theor. Appl. Genet., 1999, vol. 98, pp. 704–711.

    Article  CAS  Google Scholar 

  9. Kalendar, R. and Schulman, A.H., IRAP and REMAP for Retrotransposon-Based Genotyping and Fingerprinting, Nat. Protocols, 2006, vol. 1, no. 5, pp. 2478–2484.

    Article  CAS  Google Scholar 

  10. Leigh, F., Kalendar, R., Lea, V., et al., Comparison of the Utility of Barley Retrotransposon Families for Genetic Analysis by Molecular Marker Techniques, Mol. Gen. Genomics, 2003, vol. 269, no. 3, pp. 464–474.

    Article  CAS  Google Scholar 

  11. Bannikova, A.A., Matveev, V.A., and Kramerov, D.A., Using Inter-SINE PCR to Study Mammalian Phylogeny, Russ. J. Genet., 2002, vol. 38, no. 6, pp. 714–724.

    Article  CAS  Google Scholar 

  12. Ryabinina, N.L., Bannikova, A.A., Sheremet’eva, V.A., et al., Analysis of DNA of Higher Primates Using Inter-SINE PCR, Russ. J. Genet., 2008, vol. 44, no. 3, pp. 266–272.

    CAS  Google Scholar 

  13. Kalendar, R.N. and Glazko, V.I., Types of Molecular Genetic Markers and Their Application, Fiziol. Biokhim. Kulturnyh Rasteniy, 2002, vol. 34, no. 4, pp. 279–296.

    CAS  Google Scholar 

  14. Krasnaya kniga Permskogo kraya (Red Book of Perm Krai), Shepel’, A.I., Ed., Perm: Knizhnyi mir, 2008.

    Google Scholar 

  15. Torres, A.M., Weeden, N.F., and Martin, A., Linkage among Isozyme, RFLP and RAPD Markers in Vicia faba, Theor. Appl. Genet., 1993, vol. 5, pp. 937–945.

    Google Scholar 

  16. Kalendar, R., Tanskanen, J., Antonius-Klemola, K., et al., Cassandra Retrotransposons Carry Independently Transcribed 5S RNA, Proc. Natl. Acad. Sci. USA, 2008, vol. 105, no. 15, pp. 5833–5838.

    Article  CAS  PubMed  Google Scholar 

  17. Kalendar, R., Lee, D., and Schulman, A.H., FastPCR Software for PCR Primer and Probe Design and Repeat Search, Genes Genomes Genomics, 2009, vol. 3, no. 1, http://www.biocenter.helsinki.fi/bi/programs/fastper.htm.

  18. Poshkurlat, A.P., Rod goritsvet-Adonis L.: Sistematika, rasprostranenie, biologiya (Genus Adonis: Systematics, Distribution, Biology), Moscow: Nauka/Interperiodika, 2000.

    Google Scholar 

  19. Nei, M., Molecular Population Genetics and Evolution, Amsterdam, 1975.

  20. Kalendar, R.N. and Boronnikova, S.V., Molecular Genetic Polymorphism of Natural Populations of Ural Rare Plant Species Using Retrotransposons, Biotekhnologiya: sostoyanie i perspektivy razvitiya (Biotechnology: Current State and Perspectives of Development), Proc. 4th Moscow Int. Congress, Moscow: Ekspobiokhim-tekhnologii, 2007, part 2, p. 121.

    Google Scholar 

  21. Boyko, E., Kalendar, R., Korzun, V., et al., A HighDensity Cytogenetic Map of the Aegilops tauschii Genome Incorporating Retrotransposons and Defense Related Genes: Insights into Cereal Chromosome Structure and Function, Plant Mol. Biol., 2002, vol. 48, pp. 767–790.

    Article  CAS  PubMed  Google Scholar 

  22. Baumel, A., Ainouch, M., Kalendar, R., et al., Retrotransposons and Genomic Stability in Populations of the Young Allopolyploid Species Spartina anglica Hubard (Poaceae), Mol. Biol. Evol., 2002, vol. 19, no. 8, pp. 1218–1227.

    CAS  PubMed  Google Scholar 

  23. Kokaeva, Z.G., Boronnikova, S.V., Tikhomirova, N.N., et al., Genetic Diversity Analysis of Population Systems of Rare and Resource Plant Species, Biotekhnologiyaokhrane okruzhayushchei sredy (Biotechnology to Environment Conservation), Proc. 4th Int. Sci. Conf., in Dokl. MOIP, Moscow, 2006, pp. 75–79.

  24. Boronnikova, S.V., Molekulyarno-geneticheskaya identifikatsiya i pasportizatsiya redkikh i nakhodyashchikhsya pod ugrozoi unichtozheniya vidov rastenii (Molecular Genetic Identification and Certification of Rare and Endangered Plant Species), Perm: Perm Univ., 2008.

    Google Scholar 

  25. Boronnikova, S.V., Genetic Certification of Population of Rare Plant Species of the Genus Adonis Using ISSRand IRAP-Markers, Izv. Mosk. S-kh. Akad., 2009, no. 1, pp. 83–89.

  26. Boronnikova, S.V., Svetlakova, T.N., and Boboshina, I.V., Genetic Polymorphism Study of Populus tremula L. Using ISSRand IRAP-Markers, Agrarnaya Rossiya, 2009, no. 2, pp. 20–22.

Download references

Author information

Authors and Affiliations

  1. Biological Faculty, Department of Botany and Genetics of Plants, The Perm’ State University, Perm’, 614990, Russia

    S. V. Boronnikova

  2. Institute of Biotechnology, University of Helsinki, Helsinki, 00014, Finland

    R. N. Kalendar

Authors
  1. S. V. Boronnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. N. Kalendar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. V. Boronnikova.

Additional information

Original Russian Text © S.V. Boronnikova, R.N. Kalendar, 2010, published in Genetika, 2010, Vol. 46, No. 1, pp. 44–50.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boronnikova, S.V., Kalendar, R.N. Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants. Russ J Genet 46, 36–42 (2010). https://doi.org/10.1134/S1022795410010060

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795410010060

Keywords

Search

Navigation