Abstract
A first report on structural organization of ribosomal DNA arrays in some members of the order Squamata is presented. The data obtained point to unusually small (for vertebrates) size of the rDNA repetitive unit (approximately, 10 to 15 kb) in the lizard species examined. Analysis of BAC library of Uta stansburiana (Iguania) showed that haploid genome of this lizard contained a single cluster, consisting of about ten rDNA repeats. Determination of the extent of rDNA unit repetition in some other representatives of the order Squamata, using the method of comparative real-time PCR, showed that the number of rDNA units varied from one or several dozens in Iguanina to several hundred repeats in Scincomorpha and Varonoidea. The results are discussed in terms of an ambiguous position of the infraorder Iguania on the evolutionary trees constructed based on morphological and molecular data.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
McLysaght, A., Hokamp, K., and Wolfe, K., Extensive Genomic Duplication during Early Chordate Evolution, Nat. Genet., 2002, vol. 31, pp. 200–204.
Blanc, G. and Wolfe, K.H., Functional Divergence of Duplicated Genes Formed by Polyploidy during the Arabidopsis Evolution, Plant Cell, 2004, vol. 16, pp. 1647–1649.
Arisue, N., Hasegawa, M., and Hashimoto, T., Root of the Eukaryotic Tree Inferred from Combined Maximum Likelihood Analysis of Multiple Sequence Data, Mol. Biol. Evol., 2005, vol. 22, pp. 409–420.
Clack, J., Gaining Ground: The Origin and Evolution of Tetrapodes, Bloomington: Indiana University Press, 2002.
Garner, P., The Fossil Record of ‘Early’ Tetrapodes: Evidence of a Major Evolutionary Transition?, TJ Arch., 2003, vol. 17, no. 2, pp. 111–117.
Voronov, A.S., Shibalev, D.V., Ryskov, A.P., and Kupriyanova, N.S., Evolutional Divergence of Ribosomal Internal Transcribed Spacer 2 in Lizards, Mol. Biol., (Moscow), 2006, vol. 40, no. 1, pp. 45–51.
Mathew, C.G.P., Methods in Molecular Biology, Walker, J.M., Ed., New York: Human Press, 1984, vol. 2, pp. 31–34.
Gonzalez, I. and Sylvester, J., Complete Sequence Analysis of the 43-Kb Human Ribosomal DNA Repeat: Analysis of the Intergenic Spacer, Genomics, 1995, vol. 27, pp. 320–328.
Long, E.O. and Dawid, I.B., Repeated Genes in Eukaryotes, Annu. Rev. Biochem., 1980, vol. 49, pp. 727–764.
Kupriyanova, N.S., Timofeeva, M.Ya., Eisner, G.I., et al., Ribosomal Genes of Loach: Isolation and Restriction Analysis, Mol. Biol., (Moscow), 1981, vol. 15, no. 6, pp. 1180–1187.
Kupriyanova, N., Popenko, V., Eisner, G., et al., Organization of Loach Ribosomal Genees (Misgurnus fossilis L.), Mol. Biol. Rep., 1982, vol. 8, pp. 143–148.
Veiko, N.N., Lyapunova, N.A., Bogush, A.V., et al., Identification of Ribosomal Genes Numbers in Individual Human Genome: Comparison of the Results of Molecular and Genetic Analyses, Mol. Biol., (Moscow), 1996, vol. 30, no. 5, pp. 1076–1086.
Hedges, S.B., Moberg, K.D., and Maxon, L.R., Tetrapod Phylogeny Inferred from 18S and 28S Ribosomal RNA Sequences and a Review of the Evidence for Amniote Relationships, Mol. Biol. Evol., 1990, vol. 7, pp. 607–633.
Vidal, N. and Hedges, S.B., The Phylogeny of Squamata Reptiles (Lizards, Snakes, and Amphisbaenias) Inferred from Nine Nuclear Protein-Coding Genes, C. R. Biol., 2005, vol. 328, pp. 1000–1008.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S. Voronov, D.V. Shibaev, N.S. Kupriyanova, 2008, published in Genetika, 2008, Vol. 44, No. 11, pp. 1547–1552.
Rights and permissions
About this article
Cite this article
Voronov, A.S., Shibaev, D.V. & Kupriyanova, N.S. Specific organization of ribosomal DNA arrays in Squamata. Russ J Genet 44, 1345–1350 (2008). https://doi.org/10.1134/S1022795408110148
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795408110148