Abstract
A modification of the ISSR amplification method based on using a combination of microsatellite and specific unique primer is proposed and tested. This modification simplifies the detected PCR profiles and allows the examination of DNA regions containing definite genes. Combinations of microsatellite primer Mic2 (5′-gacag-acaga-cagac-a-3′) and one of the primers specific to the Adh1 locus, which controls alcohol dehydrogenase (ADH1) in sugar beet, were employed in this work. The microsatellite primer was used in combination with the following specific primers: Adh1f (5′-agagt-gttgg-agagg-gtgtg-ac-3′) containing the binding site at the fourth exon of gene Adh1, or Adh1r (5′-act(ct)a-cagca-ag(ct)cc-(ct)ac(ct)g-ctcc-3′) that binds to the fifth exon of the same gene. In the agamospermous progeny of individual heterozygous diploid plants of sugar beet with the Adh1-F/Adh1-S genotype, polymorphism of PCR profiles obtained in plants of each of three phenotypic classes (FF, FS, and SS) was detected. Among plants of the progeny from an individual plant that represents the heterozygous phenotypic class FS, differences were revealed not only between the PCR profiles but also in the relative activity of allele isozymes of ADH1.
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References
Sandaltzopoulos, R., Mitchelmore, C., Bonte, E., et al., Dual Regulation of the Drosophila hsp26 Promoter in vitro, Nucleic Acids Res., 1995, vol. 23, pp. 2479–2487.
Punt, P.J., Dingemanse, M.A., Kuyvenhoven, A., et al., Functional Elements in the Promoter Region of the Aspergillus nidulans gpdA Gene Coding Glyceraldehyde-3-Phosphate Dehydrogenase, Gene, 1990, vol. 93, pp. 101–109.
Chen, Y. and Roxby, R., Identification of a Functional CT-Element in the Phytophthora infestans piypt1 Gene Promoter, Gene, 1997, vol. 198, nos. 1–2, pp. 159–164.
Vinichenko, N.A., Kirikovich, S.S., and Levites, E.V., Modification of the ISSR Amplification Technique for the Analysis of the Adh1 Allele Variation Patterns in Agamospermous Progeny of Sugar Beet, Faktori Eksperimental’noi Evolyutsii Organizmiv (Factors of Experimental Evolution of the Organisms), Kiiv: “Logos”, 2006, vol. 3, pp. 80–84.
Levites, E.V., Shkutnik, T., Ovechkina, O.N., and Maletskii, S.I., Pseudosegregation in Agamospermous Progeny of Pollen-Sterile Sugar Beet Plants (Beta vulgaris L.), Dokl. Akad. Nauk, 1998, vol. 362, no. 3, pp. 430–432.
Levites, E.V., Redetermination: An Interesting Epigenetic Phenomenon Associated with Mitotic Agamospermy in Sugar Beet, Sugar Tech., 2002, vol. 4, vol. 3&4, pp. 137–141.
Levites, E.V. and Kirikovich, S.S., Epigenetic Variability of Unlinked Enzyme Genes in Agamospermous Progeny of Sugar Beet, Sugar Tech., 2003, vol. 1&2, pp. 57–59.
Maletskii, S.I. and Konovalov, A.A., Inheritance of Alcohol Dehydrogenase in Sugar Beet: 1. Analysis of the Deviation from Monogenic Segregation, Genetika (Moscow), 1985, vol. 21, no. 9, pp. 1527–1540.
Vinichenko, N.A., Golovnina, K.A., Blinov, A.G., et al., Molecular Differences between Alleles Adh1-F and Adh1-S in Sugar Beet Beta vulgaris L., Russ. J. Genet., 2004, vol. 40, no. 2, pp. 172–177.
Schwartz, D., The Genetic Control of Alcohol Dehydrogenase in Maize: Gene Duplication and Repression, Proc. Natl. Acad. Sci. USA, 1966, vol. 56, pp. 1431–1436.
Levites, E.V., Genetika izofermentov rastenii (Genetics of Plant Isozymes), Novosibirsk: Nauka, 1986.
Doyle, J.J. and Doyle, J.L., A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.
Woodman, J.G. and Freeling, M., Identification of a Genetic Element That Controls the Organ-Specific Expression of Adh1 in Maize, Genetics, 1981, vol. 98, no. 2, pp. 357–378.
Schmidt, T. and Heslop-Harrison, J.S., The Physical and Genomic Organization of Microsatellites in Sugar Beet, Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 8761–8765.
Stallings, R.L., Ford, A.F., Nelson, D., et al., Evolution and Distribution of (GT)n Repetitive Sequences in Mammalian Genomes, Genomics, 1991, vol. 10, pp. 807–815.
Templeton, A.R., Clark, A.G., Weiss, K.M., et al., Recombinational and Mutational Hot Spots within the Human Lipoprotein Lipase Gene, Am. J. Hum. Genet., 2000, vol. 66, pp. 69–83.
Jeffreys, A.J., Murray, J., and Neumann, R., High-Resolution Mapping of Crossovers in Human Sperm Defines a Minisatellite-Associated Recombination Hotspot, Mol. Cell, 1998, vol. 2, pp. 267–273.
Eisen, J.A, Mechanistic Basis of Microsatellite Instability in Microsatellites: Evolution and Applications, in Microsatellites: Evolution and Applications, Goldstein, D.B. and Schlotterer, C., Eds., Oxford: Oxford Univ. Press, 1999, pp. 34–48.
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Original Russian Text © N.A. Vinichenko, S.S. Kirikovich, E.V. Levites, 2008, published in Genetika, 2008, Vol. 44, No. 9, pp. 1252–1256.
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Vinichenko, N.A., Kirikovich, S.S. & Levites, E.V. Polymorphism of PCR profiles and expression of alleles at the locus Adh1 in agamospermous progeny of sugar beet Beta vulgaris L.. Russ J Genet 44, 1092–1095 (2008). https://doi.org/10.1134/S1022795408090123
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DOI: https://doi.org/10.1134/S1022795408090123