Skip to main content
Log in

Production of potato breeding material using somatic hybrids between Solanum tuberosum L. dihaploids and the wild diploid species Solanum bulbocastanum Dunal. from Mexico

  • Plant Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

An original approach to overcome interspecific incompatibility when backcrossing the tetraploid Solanum tuberosum + S. bulbocastanum somatic hybrids with cultivated potato was realized. This method is based on the decrease in their ploidy using anther culture and involvement of the haploid producer S. phureja IvP35. The feasibility of obtaining a diploid progeny from the somatic hybrids carrying genetic material of the wild species S. bulbocastanum and crossable with S. tuberosum dihaploids was demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Yermishin, A.P., Makhanko, O.V., Voronkova, E.V., and Khlebnikova, L.M., Usage of Somatic Hybrids between Dihaploids of Solanum tuberosum L. and Wild Diploid Potato Species from Mexico in Breeding, in The Biology of Plant Cells in Vitro and Biotechnology, Abstr. 8th Int. Conf., Saratov, 2003, p. 369.

  2. Yermishin, A.P., Khlebnikova, L.M., Voronkova, E.V., and Makhanko, O.V., Dihaploids (2n = 2x = 24) of Somatic Hybrids tbr + pnt (2n = 4x = 48) as a Tool for the Introduction of Wild Species Germplasm into Potato Breeding Material, Abstr. 15th Triennial Conf. EAPR, Hamburg, 2002.

  3. Yermishin, A.P., Makhanko, O.V., and Voronkova, E.V., Application of Somatic Hybrids between Dihaploids of Potato Solanum tuberosum L. and Wild Diploid Species from Mexiso in Breeding: Generation and Backcrossing of Dihaploids of Somatic Hybrids, Russ. J. Genet., 2006, vol. 42, no. 12, pp. 1414–1421.

    Article  CAS  Google Scholar 

  4. Makhanko, O.V., Voronkova, E.V., Kozlov, V.A., et al., Influence of Different Incompatibility Types on the Hybridization Results of Wild Diploid Potato Species with Dihaploids of Solanum tuberosum, Otdalennaya gibridizatsiya: Sovremennoe sostoyanie i perspektivy razvitiya (Distant Hybridization: Current State and Perspectives of Development), Proc. Int. Conf. Distant Hybridization, Moscow, 1: Izd. Mosk. S-kh. Akad., 2003, pp. 161–165.

    Google Scholar 

  5. Schilde-Rentschler, L., Boos, G., and Ninnemann, H., Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding, Progress in Plant Protoplast Research, Dordrecht: Kluwer, 1988, pp. 195–196.

    Google Scholar 

  6. Voronkova, E.V., Approaches to Enhance Androgenic Ability of Potato and Genetic Characteristics of Androregenerants, Cand. Sci. (Biol.) Dissertation, Minsk, 1999.

  7. Nitsch, J.P. and Nitsch, C., Haploid Plants from Pollen Grains, Science, 1969, vol. 163, no. 3862, pp. 85–87.

    Article  PubMed  Google Scholar 

  8. Murashige, T. and Skoog, F., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant., 1962, vol. 15, no. 13, pp. 473–497.

    Article  CAS  Google Scholar 

  9. Pausheva, Z.P., Praktikum po Tsitologii Rastenii (Manual in Plant Cytology), Moscow: Kolos, 1974.

    Google Scholar 

  10. Kostrica, P. and Domkarova, J., Possibilities of Indirect Determination of the Ploidy in Potato Regenerants in vitro, Rostlinna Viroba UZPI, 1993, vol. 39, no. 2, pp. 139–148.

    Google Scholar 

  11. Hermsen, J.G.Th. and Verdenius, J., Selection from Solanum tuberosum Group Phureja of Genotypes Combining High Frequency Haploid Induction with Homozygosity for Embryo-Spot, Euphytica, 1973, vol. 22, pp. 244–259.

    Article  Google Scholar 

  12. Sivolap, Yu.M., Solodenko, A.E., and Burlov, V.V., RARD Analysis of Molecular-Genetic Polymorphism in Sunflower Helianthus annuus, Russ. J. Genet., 1998, vol. vol. 34, no. 2, pp. 199–203.

    CAS  Google Scholar 

  13. Shure, M., Wessler, S., and Fedoroff, N., Molecular Identification and Isolation of the Waxy Locus in Maize, Cell, 1983, vol. 35, pp. 225–233.

    Article  PubMed  CAS  Google Scholar 

  14. Rokka, V.-M., Androgenic Haploidization and Interspecific and Intraspecific Somatic Hybridization in Potato Germplasm Development, Acad. Dissertation, Helsinki, 1998, p. 62.

  15. Yermishin, A.P. and Voronkova, E.V., Sposob polucheniya digaploidov kartofelya Solanum tuberosum L. (Method for Production of Potato Solanum tuberosum L. Dihaploids), Avtorskoye Svidetel’stov SSSR, no. 1554368, 1968.

  16. Meyer, R., Salamini, F., and Uhrig, H., Isolation and Characterization of Potato Diploid Clones Generating a High Frequency of Monohaploid or Homozygous Diploid Androgenetic Plants, Theor. Appl. Genet., 1993, vol. 85, pp. 905–912.

    Article  Google Scholar 

  17. Kumar, A., Somaclonal Variation, Potato Genetics, Bradshaw, J.E. and Mackay, G.R., Eds., Wallingford: CAB, 1994, pp. 197–213

    Google Scholar 

  18. Voronkova, E.V., Cytogenetic Instability in Anther Culture Derived Regenerated Plants of Potato S. tuberosum L. and Their Sexual and Vegetative Generations, in The Biology of Plant Cells in Vitro and Biotechnology, Abstr. 8th Int. Conf., Saratov, 2003, p. 352.

  19. Jacobsen, E., Doubled Dihaploid Potato Clones via Leaf Tissue Culture, Zeitschrift Pflanzenzuchtung, 1977, vol. 80, pp. 80–82.

    Google Scholar 

  20. Hermsen, J.G.Th., Ramanna, M.S., Roest, S., and Bokelmann, G.S., Chromosome Doubling through Adventitious Shoot Formation on in vitro Cultivated Leaf Explants from Diploid Interspecific Potato Hybrids, Euphytica, 1981, vol. 30, pp. 239–246.

    Article  Google Scholar 

  21. Peloquin, S.J., Yerk, G.L., Werner, J.E., and Darmo, E., Potato Breeding with Haploids and 2n Gametes, Genome, 1989, vol. 31, pp. 1000–1004.

    Google Scholar 

  22. Jansky, S.H., Peloquin, S.J., and Yerk, G.L., Use of Potato Haploids to Put 2x Wild Species Germplasm in Usable Form, Plant Breed., 1990, vol. 104, pp. 290–294.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. P. Yermishin.

Additional information

Original Russian Text © A.P. Yermishin, O.V. Makhan’ko, E.V. Voronkova, 2008, published in Genetika, 2008, Vol. 44, No. 5, pp. 645–653.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yermishin, A.P., Makhan’ko, O.V. & Voronkova, E.V. Production of potato breeding material using somatic hybrids between Solanum tuberosum L. dihaploids and the wild diploid species Solanum bulbocastanum Dunal. from Mexico. Russ J Genet 44, 559–566 (2008). https://doi.org/10.1134/S1022795408050086

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795408050086

Keywords

Navigation