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Production of Hybrids Between the 2EBN Bridge Species Solanum verrucosum and 1EBN diploid Potato Species

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Abstract

The potato crop has diploid wild relatives in the primitive 1EBN crossability group that have a wealth of desirable traits, but are currently difficult to access through conventional crossing. The objective of this study was to develop an efficient technique for using 1EBN species in breeding by crossing to 2EBN bridge species S. verrucosum. Success was obtained with several 1EBN diploid species: S. bulbocastanum, S. pinnatisectum, S. polyadenium, S. commersonii and S. circaeifolium. Use of 2x(2EBN) S. verrucosum as a receptive female avoided prezygotic interspecific incompatibility, and double “rescue” pollination was done using haploid-inducing clone, S. phureja IvP35 to minimize postzygotic failure due to abortion of berries with few or small seeds. In total, 4,646 hybrid seeds were obtained in crosses between S. verrucosum and the 1EBN species. Rescue pollination particularly improved seeds per pollination for S. pinnatisectum hybrids. The hybrid seeds were normal in appearance and had high germination (47–88 %). They produced plants with the distinctive phenotypic characteristics and molecular markers specific to their 1EBN parents. Hybrids had poor male fertility, but crossed easily as females to diploid S. tuberosum at an average of 38 seeds per pollination.

Resumen

El cultivo de la papa tiene especies diploides silvestres cercanas en el grupo primitivo de cruzabilidad 1EBN con abundancia de caracteres deseables, pero con dificultades actuales de accesibilidad por la vía de los cruzamientos convencionales. El objetivo de este estudio fue desarrollar una técnica eficiente para el uso de las especies 1EBN en mejoramiento mediante el cruzamiento a la especie-puente 2EBN S. verrucosum. Se obtuvo éxito con varias especies diploides 1EBN: S. bulbocastanum, S. pinnatisectum, S. polyadenium, S. commersonii y S. circaeifolium. El uso de S. verrucosum, que es 2x(2EBN), como hembra receptiva, evitó la incompatibilidad pre-cigótica interespecífica, y se hizo una doble polinización de “rescate” usando un clon inductor de haploide, S. phureja IvP35, para minimizar falla postcigótica debida a la aborción de frutos con pocas o pequeñas semillas. En total se obtuvieron 4,646 semillas híbridas en cruzas entre S. verrucosum y las especies 1EBN. El rescate de la polinización mejoró particularmente las semillas por polinización para híbridos de S. pinnatisectum. Las semillas híbridas fueron aparentemente normales y tuvieron alta germinación (47–88 %). Produjeron plantas con las características fenotípicas peculiares y marcadores moleculares específicos para sus padres 1EBN. Los híbridos tuvieron pobre fertilidad masculina, pero se cruzaron fácilmente como hembras con S. tuberosum diploide en un promedio de 38 semillas por polinización.

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Acknowledgments

The authors thank E. Kuzminova (Centre “Genome” of the Institute of Genetics and Cytology) for help with SSR-analysis, Prof. E. Khavkin (Institute of Agricultural Biotechnology, Moscow, Russia) for providing the information on DNA-markers, and K. Pavur (International House, Minsk) for editing the manuscript.

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  1. Institute of Genetics and Cytology, Belarusian National Academy of Sciences, 27 Akademicheskaya Street, Minsk, 220072, Belarus

    A. P. Yermishin, Yu. V. Polyukhovich, E. V. Voronkova & A. V. Savchuk

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  1. A. P. Yermishin
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  2. Yu. V. Polyukhovich
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  3. E. V. Voronkova
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  4. A. V. Savchuk
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Correspondence to A. P. Yermishin.

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Yermishin, A.P., Polyukhovich, Y.V., Voronkova, E.V. et al. Production of Hybrids Between the 2EBN Bridge Species Solanum verrucosum and 1EBN diploid Potato Species. Am. J. Potato Res. 91, 610–617 (2014). https://doi.org/10.1007/s12230-014-9385-9

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