Abstract
In the exponentially growing E. coli cells we have described in 2005 a new fundamental genetic phenomenon, namely quasi-adaptive response to alkylating compounds, “quasi-Ada”. Phenotypic expression of “quasi-Ada” is similar to the true Ada response, however in contrast it develops in the course of pretreatment of the cells by sublethal dose of non-alkylating agent, an NO-containing dinitrosyl iron complex with glutathione (DNICglu). To reveal the mechanisms of quasi-adaptation and its association with the function of the regulatory protein Ada here we used a unique property of dual gene expression regulation of aidB1 gene, a part of Ada-regulon, namely its relative independence from Ada protein in anaerobic conditions. Based on the results of aidB1 gene expression analysis an EPR spectra of E.coli MV2176 cells (aidB1::lacZ) in aerobic and anaerobic conditions after the corresponding treatments we concluded that the function and the spatial structure of meAda and [(Cys−)2Fe+(NO+)2]Ada are identical and thus the nitrosylated protein represents an Ada regulon genes expression regulator during quasi-adaptation development.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Landini, P., Hajec, L.I., and Volkert, M.R., Structure and Transcriptional Regulation of the Escherichia coli Adaptive Response Gene aidB, J. Bacteriol., 1994, vol. 276, pp. 6583–6589.
Smirnova, G.V., Oktyabrsky, O.N., Moshonkina, E.V., et al., Induction of the Alkylation-Inducible aidB Gene of Escherichia coli by Cytoplasmic Acidification and N-Ethylmaleimide, Mutat. Res., 1994, vol. 314, pp. 51–56.
Vasileva, S.V. and Moshkovskaya, E.Yu., Quasi-Adaptive Response to Alkylating Agents in Escherichia coli, Russ. J. Genet., 2005, vol. 41, no. 5, pp. 484–489.
Vasileva, S.V., Moshkovskaya, E.Yu., Sanina, N.A., et al., The Formation of Dinitrosyl Iron Complexes—a Necessary Stage in Realization of Genetic Activity of Tetranitrozyl-Tiosulphate Complex [Na2[Fe2(m-S2O3)2(NO)4] · 4H2O, Dokl. RAN, 2005, vol. 402, no. 5, pp. 1–4.
Vasileva, S.V., Makhova, E.V., and Moshkovskaya, E.Yu., Expression and Functions of Adaptive Response Genes in Escherichia coli Treated with Mono-and Bifunctional Alktlating Agents: Interference with SOS Response, Russ. J. Genet., 1999, vol. 35, no. 4, pp. 364–369.
Miller, J.H., Experiments in Molecular Genetics, New York: Cold Spring Harbor Lab., 1972.
Mackay, W.J., Han, S., and Samson, L.D., DNA Alkylation Repair Limits Spontaneous Base Substitution Mutations in Escherichia coli, J. Bacteriol., 1994, vol. 176, pp. 3224–3230.
Taverna, P. and Sedgwick, B., Generation of an Endogenous DNA-Methylating Agent by Nitrosation in Escherichia coli, J. Bacteriol., 1996, vol. 178, pp. 5105–5111.
Landini, P. and Volkert, M.R., Regulatory Responses of the Adaptive Response to Alkylation Damage: A Simple Regulon with Complex Regulatory Features, J. Bacteriol., 2000, vol. 182, no. 23, pp. 6543–6549.
Farewell, A., Kvint, K., and Nystrom, T., Negative Regulation by RpoS: A Case of Sigma Factor Competition, Mol. Microbiol., 1998, vol. 29, pp. 1039–1051.
Frolov, E.N. and Vanin, A.F., A Novel Type of Paramagnetic Nitrosyl Iron Complexes of Nonhemic Iron, Biofizika, 1973, vol. 18, pp. 605–610.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S.V. Vasilieva, E.Yu. Moshkovskaya, A.S. Terekhov, V.D. Mikoyan, A.F. Vanin, 2008, published in Genetika, 2008, Vol. 44, No. 1, pp. 29–35.
Rights and permissions
About this article
Cite this article
Vasilieva, S.V., Moshkovskaya, E.Y., Terekhov, A.S. et al. Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli . Russ J Genet 44, 21–26 (2008). https://doi.org/10.1134/S1022795408010031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1134/S1022795408010031