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Viable nonsense mutants for the SUP45 gene in the yeast Saccharomyces cerevisiae are lethal at increased temperature

  • Genetics of Microorganisms
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Abstract

Nonlethal nonsense mutations obtained earlier in the essential gene SUP45 encoding the translation termination factor eRF1 in the yeast Saccharomyces cerevisiae were further characterized. Strains carrying these mutations retain the viability, since the full-length eRF1 protein is present in these strains, although in decreased amounts as compared to wild-type cells, together with a trucated eRF1. All nonsense mutations are likely to be located in a weak termination context, because a change in the stop codon UGAA (in the case of mutation sup45-107) to UAGA (sup45-107.2) led to the alteration of the local context from a weak to strong and to the lethality of the strain carrying sup45-107.2. All nonsense mutations studied are characterized by thermosensitivity expressed as cell mortality after cultivation at 37°C. When grown under nonpermissive conditions (37°C), cells of nonsense mutants sup45-104, sup45-105, and sup45-107 display a decrease in the amount of the truncated eRF1 protein without reduction in the amount of the full-length eRF1 protein. The results of this study suggest that the N-terminal eRF1 fragment is indispensable for cell viability of nonsense mutants due to the involvement in termination of translation.

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Authors and Affiliations

  1. Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 199034, Russia

    G. A. Zhouravleva, S. E. Moskalenko, O. A. Murina & S. G. Inge-Vechtomov

  2. CNRS UMR 6061 Génétique et Développement, Université de Rennes 1, IFR 140, Faculté de Médicine, 2 av. Pr. Leon Bernard, CS 34317, 35043, Rennes Cedex, France

    G. A. Zhouravleva & S. E. Moskalenko

  3. St. Petersburg Branch of Vavilov Institute of General Genetics, St. Petersburg, 199034, Russia

    S. G. Inge-Vechtomov

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  1. G. A. Zhouravleva
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  2. S. E. Moskalenko
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  3. O. A. Murina
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  4. S. G. Inge-Vechtomov
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Original Russian Text © G.A. Zhouravleva, S.E. Moskalenko, O.A. Murina, S.G. Inge-Vechtomov, 2007, published in Genetika, 2007, Vol. 43, No. 10, pp. 1363–1371.

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Zhouravleva, G.A., Moskalenko, S.E., Murina, O.A. et al. Viable nonsense mutants for the SUP45 gene in the yeast Saccharomyces cerevisiae are lethal at increased temperature. Russ J Genet 43, 1139–1146 (2007). https://doi.org/10.1134/S1022795407100079

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  • DOI: https://doi.org/10.1134/S1022795407100079

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