Abstract
Using the model of synthetic allopolyploid Aegilops longissima TL05 × Triticum urartu TMU06 of the first generation, the degree and character of changes in subtelomeric, microsatellite and random amplified DNA sequences (RAPD) on early stage of polyploidization was estimated. Study of genome changes was performed by comparing PCR fragments of the allopolyploid and its parental forms. For analysis of subtelomeric DNA, we used 66 pairs of primers composed of 11 singular primers designed for these chromosomal regions sequences of cereals. RAPD analysis was performed with usage of 38 primers, in microsatellite (SSR) analysis 23 primer pairs were used. RAPD analysis appeared to be a more effective PCR-based method to identify genome changes. Absence of some RAPD fragments typical for parental genome in allopolyploid TL05 × TMU06 was shown using 13 primers of 38 (34%), and with usage of subtelomeric primers the changes in PCR fragments were shown only for one of 66 pairs of primers (1.5%). SSR loci were stable during the polyploidization process. Subsequent analysis of PCR fragments absent in the synthetic allopolyploid showed that high level of genome changes in RAPD analysis is probably connected with more effective ability of this method to reveal point mutations. Some data was found suggesting that not all genome changes observed in experimentally synthesized allopolyploids of the first generation are consequences of coadaptation of few genomes in one nucleus.
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References
Soltis, D.E. and Soltis, P.S., The Dynamic Nature of Polyploid Genomes, Proc. Natl. Acad. Sci. USA, 1995, vol. 92, pp. 8089–8091.
Wendel, J.F., Genome Evolution in Polyploids, Plant. Mol. Biol., 2000, vol. 42, pp. 225–249.
Axelsson, T., Bowman, C.M., Sharpe, A.G., et al., Amphidiploid Brassica juncea Contains Conserved Progenitor Genomes, Genome, 2000, vol. 43, pp. 679–688.
Skalicka, K., Lim, K.Y., Matyasek, R., et al., Preferential Elimination of Repeated DNA Sequences from the Paternal, Nicotiana tomentosiformis Genome Donor of a Synthetic Allotetraploid Tobacco, New Phytol., 2005, vol. 166, pp. 291–303.
Liu, B., Brubaker, C.L., Mergeai, G., et al., Polyploid Formation in Cotton Is Not Accompanied by Rapid Genomic Changes, Genome, 2001, vol. 44, pp. 321–330.
Levy, A. and Feldman, M., Genetic and Epigenetic Reprogramming of the Wheat Genome upon Allopolyploidisation, Biol. J. Linnaean Soc., 2004, vol. 82, pp. 607–613.
Ozkan, H., Levy, A.A., and Feldman, M., Allopolyploidy-Induced Rapid Genome Evolution in the Wheat (Aegilops-Triticum) Group, Plant Cell, 2001, vol. 13, pp. 1735–1747.
Shaked, H., Kashkush, K., Ozkan, H., et al., Sequence Elimination and Cytosine Methylation Are Rapid and Reproducible Responses of the Genome to Wide Hybridization and Allopolyploidy in Wheat, Plant Cell, 2001, vol. 13, pp. 1749–1759.
Kashkush, K., Feldman, M., and Levy, A.A., Transcriptional Activation of Retrotransposons Alters the Expression of Adjacent Genes in Wheat, Nat. Genet., 2003, vol. 33, pp. 102–106.
Salina, E.A., Numerova, O.M., Ozkan, H., and Feldman, M., Alterations in Subtelomeric Tandem Repeats during Early Stages of Allopolyploidy in Wheat, Genome, 2004, vol. 47, pp. 860–867.
Levy, A.V. and Feldman, M., The Impact of Polyploidy on Grass Genome Evolution, Plant Physiol., 2002, vol. 130, pp. 1587–1593.
Salina, E.A., Lim, Y.K., Badaeva, E.D., et al., Phylogenetic Reconstruction of Aegilops Section Sitopsis and the Evolution of Tandem Repeats in the Diploids and Derived Wheat Polyploids, Genome, 2006, vol. 49, pp. 1023–1035.
Salina, E., Adonina, I., Vatolina, T., and Kurata, N., A Comparative Analysis of the Composition and Organization of Two Subtelomeric Repeat Families in Aegilops speltoides Tausch. and Related Species, Genetics, 2004, vol. 122, pp. 227–237.
Roeder, M.S., Korzun, V., Wendehake, K., et al., A Microsatellite Map of Wheat, Genetics, 1998, vol. 149, pp. 2007–2023.
Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory, Manual, New York: Cold Spring Harb. Lab., 1982.
Altchul, S.F., Gish, W., Miller, W., et al., Basic Local Alignment Search Tool, J. Biol., 1990, vol. 215, pp. 403–410.
Feinberg, A.P. and Vogelstein, B.A., A Technique for Radiolabelling DNA Restriction Endonuclease Fragments to High Specific Activity, Anal. Biochem., 1983, vol. 132, pp. 6–13.
Adonina, I.G., Salina, E.A., Pestsova, E.G., and Roeder, M.S., Transferability of Wheat Microsatellites to Diploid Aegilops Species and Determination of Chromosomal Localizations of Microsatellites in the S Genome, Genome, 2005, vol. 48, pp. 959–970.
Salina, E.A., Leonova, I.N., Efremova, T.T., and Roeder, M.S., Wheat Genome Structure: Translocations during the Course of Polyploidization, Funct. Integr. Genomics, 2006, vol. 6, pp. 71–80.
Penner, G.A, RARD Analysis Plant Genome, Method of Genome Analysis in Plants, Jauhar, P.P., Ed., New York: CRC, 1996, pp. 252–264.
Bildanova, L.L., Salina, E.A., and Pershina, L.A., Investigation of the Backcross Progeny of Barley-Wheat Hybrids by the RARD and RAMRO Methods, Russ. J. Genet., 2002, vol. 38, pp. 257–263.
Schlotterer, C., Evolutionary Dynamics of Microsatellite DNA, Chromosoma, 2000, vol. 109, pp. 365–371.
Palomi, M.A. and Damiano, C., Comparison between RAPD and SSR Molecular Markers in Detecting Genetic Variation in Kiwifruit (Actinidia deliciosa A. Chev.), Plant Cell Rep., 2002, vol. 21, pp. 1061–1066.
Rahman, M.H. and Rajora, O.P., Microsatellite DNA Somaclonal Variation in Micropropagated Trembling Aspen (Populus tremuloides), Plant Cell Rep., 2001, vol. 20, pp. 531–536.
Dobrovol’skaya, O.B., Characterization of Wheat-Rye Translocation Lines Using Microsatellite Markers and the Study of Individual Rye Chromosome Influence on Androgenesis Indexes in Vitro, Cand. Sci. (Biol.) Dissertation, Novosibirsk: Inst. Cytol. Genet., Rus. Acad. Sci., 2003.
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Original Russian Text © A.B. Shcherban, E.K. Khlestkina, E.M. Sergeeva, E.A. Salina, 2007, published in Genetika, 2007, Vol. 43, No. 7, pp. 963–970.
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Shcherban, A.B., Khlestkina, E.K., Sergeeva, E.M. et al. Genomic changes at early stages of formation of allopolyploid Aegilops longissima × Triticum urartu . Russ J Genet 43, 798–804 (2007). https://doi.org/10.1134/S1022795407070125
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DOI: https://doi.org/10.1134/S1022795407070125