Abstract
Development of Drosophila imaginal discs is accompanied by a high-ordered cell proliferation. However, the distinctions in the topographic distribution of mitoses at different developmental stages are insufficiently studied. In this work, we have analyzed the distribution of mitoses in the wing disc of third-instar larvae and determined the regions where mitotic clustering. The results obtained demonstrate that the proliferation rate is region-specific, which is determined by the location of cell cycle regulators and/or the location of growth factors. A comparison of the topography of mitoses with the activity patterns of the regulatory regions of gene string (stg), a known regulator of the mitotic M phase, has demonstrated a similarity between the topography and the activity pattern of one of these regions. The similarity between mitotic distributions in the left and right discs of the same larva (compared with the similarity of gene neuralized expression patterns is considered, and the degree of histone H3 phosphorylation at various mitotic stages is analyzed.
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References
Garcia-Bellido, A. and Merriam, J.R., Parameters of the Wing Imaginal Disc Development of Drosophila melanogaster, Dev. Biol., 1971, vol. 24, pp. 61–87.
Gonzalez-Gaitan, M., Capdevila, M.P., and Garcia-Bellido, A., Cell Proliferation Patterns in the Wing Imaginal Disc of Drosophila, Mech. Dev., 1994, vol. 40, pp. 183–200.
Dubatolova, T. and Omelyanchuk, L., Analysis of Cell Proliferation in Drosophila Wing Imaginal Disc Using Mosaic Clones, Heredity, 2004, vol. 92, pp. 299–305.
Resino, J., Salama-Cohen, P., and Garcia-Bellido, A., Determining the Role of Patterned Cell Proliferation in the Shape and Size of the Drosophila Wing, Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 7502–7507.
Milan, M., Campuzano, S., and Garcia-Bellido, A., Cell Cycling and Patterned Cell Proliferation in the Wing Primordium of Drosophila, Proc. Natl Acad. Sci. USA, 1996, vol. 93, pp. 640–645.
Lehman, D.A., Patterson, B., Johnston, L.A., et al., Cis Regulatory Elements of the Mitotic Regulator, String/Cdc25, Development, 1999, vol. 126, pp. 1793–1803.
Modolell, J. and Campuzano, S., The Achaete-Scute Complex as an Integrating Device, Int. J. Dev. Biol., 1998, vol. 42, pp. 275–282.
Baena-Lopez, L.A., Baonza, A., and Garcia-Bellido, A., The Orientation of Cell Divisions Determines the Shape of Drosophila Organs, Curr. Biol., 2005, vol. 15, pp. 1640–1644.
Jonston, L.A. and Edgar, B., Wingless and Notch Regulate Cell-Cycle Arrest in the Developing Drosophila Wing, Nature, 1998, vol. 394, pp. 82–84.
Huang, F., Dambly-Chaudiere, C., and Ghysen, A., The Emergence of Sence Organs in the Wing Disc of Drosophila, Development, 1991, vol. 111, pp. 1087–1095.
Milan, M., Campuzano, S., and Garcia-Bellido, A., Cell Cycling and Patterned Cell Proliferation in the Drosophila Wing during Metamorphosis, Proc. NatlAcad. Sci. USA, 1996, vol. 93, pp. 687–692.
Negre, N., Ghysen, A., and Martinez, A.M., Mitotic G2-Arrest Is Required for Neural Cell Fate Determination in Drosophila, Mech. Dev., 2003, vol. 120, pp. 253–265.
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Original Russian Text © L.V. Omel’yanchuk, C. Nokkala, J. Mattila, L.I. Lebedeva, T.Yu. Baimak, K.A. Akhmetova, 2007, published in Genetika, 2007, Vol. 43, No. 7, pp. 930–937.
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Omel’yanchuk, L.V., Nokkala, C., Mattila, J. et al. The distribution of mitoses in the imaginal disks of third-instar Drosophila melanogaster larvae. Russ J Genet 43, 769–775 (2007). https://doi.org/10.1134/S1022795407070083
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DOI: https://doi.org/10.1134/S1022795407070083