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Effects of Nanoparticles on Activity of Lignan Biosynthesis Enzymes in Cell Suspension Culture of Linum usitatissimum L.

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Abstract

Flax (Linum usitatissimum L.), as an important commercial crops, is a rich source of fatty acids, protein, dietary fiber and lignans. Flax plant has an effective anticancer activity due to lignan contents. Nanoparticles have recently been used as efficient non-biologic elicitors to improve the biosynthesis of secondary metabolites. In this study, the effects of different concentration of ZnO (0, 30, 60, and 120 mg/L) and TiO2 (0, 50, 100, and 150 mg/L) nanoparticles at different time (0, 24, 48, and 72 h) were investigated on enzyme activities and production of secondary metabolites in cell suspension cultures of flax. The results indicated that the highest activity of phenylalanine ammonia lyase (PAL) was observed in 30 mg/L nano-ZnO treatment at 48 h, whereas the effect of nano-TiO2 on PAL enzyme activity was not statistically significant. According to the results, the highest activity of CAD (cinnamyl alcohol dehydrogenase) was observed in 60 mg/L concentration of nano-ZnO at different intervals. The use of 150 mg/L nano-TiO2 led to increased activity of CAD. The maximum content of total phenol was detected at 150 mg/L nano-TiO2. Different concentrations of nano-TiO2, caused to an increase in total lignan at all intervals. The highest amount of total phenol and lignans was observed in 30 and 60 mg/L ZnO. In the present study, we were observed different effects of nanoparticle on enzymes activity and secondary metabolite production in cell suspension cultures of flax plant, depending on concentration and type of nanoparticles.

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ACKNOWLEDGMENTS

Appreciation to all staff for their collaboration towards the success of this work, which was carried out at the Biotechnology Department of Imam Khomeini International University.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran

    F. Karimzadeh, R. Haddad & Gh. Garoosi

  2. Department of Genetic and Plant Breeding, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran

    R. Khademian

Authors
  1. F. Karimzadeh
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  2. R. Haddad
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  3. Gh. Garoosi
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  4. R. Khademian
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Correspondence to F. Karimzadeh.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Abbreviations: CAD—cinnamyl alcohol dehydrogenase; SCV— settled cell volume; SDG—secoisolariciresinol diglucoside.

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Karimzadeh, F., Haddad, R., Garoosi, G. et al. Effects of Nanoparticles on Activity of Lignan Biosynthesis Enzymes in Cell Suspension Culture of Linum usitatissimum L.. Russ J Plant Physiol 66, 756–762 (2019). https://doi.org/10.1134/S1021443719050078

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