Abstract
Verticillium wilt (V. wilt), a notorious wilt disease caused by Verticillium dahliae, often leads to the reduction of eggplant (Solanum melongena L.) production. MiRNAs, as a class of small RNAs, can regulate gene expression and then affect growth and development in plants. MiR395 has been proven to respond to sulfate-deficient stress in Arabidopsis thaliana and sulfate is well known to have a close relationship with plant disease resistance. To explore the function of eggplant miR395, we examined its expression in V. dahliae-infected eggplant by qRT-PCR and found miR395 exhibited a gradual reduction trend with time after infection. We then expressed pre-miR395 from Arabidopsis thaliana in Suqi eggplant and resistance analysis showed that miR395 overexpressed plants were hypersensitive to V. dahliae infection. We further measured the content of GSH and activities of POD and SOD and the results indicated that the index of GSH/POD/SOD in the overexpressed plants was lower than that of the wild-type control under V. dahliae infection. These results suggest that miR395 plays a negative role in eggplant response to V. dahliae infection.
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Abbreviations
- 6-BA:
-
6-benzylaminopurine
- Cef:
-
cefotaxime
- CTAB:
-
hexadecyl trimethyl ammonium bromide
- GSH:
-
glutathione
- Hyg B:
-
hygromycin B
- KT:
-
kinetin
- POD:
-
peroxidase
- qRT-PCR:
-
real-time quantitative RT-PCR
- RISC:
-
RNAinduced silencing complex
- SOD:
-
superoxide dismutase
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Mu, X.Y., Liu, X.R., Cai, J.H. et al. MiR395 Overexpression Increases Eggplant Sensibility to Verticillium dahliae Infection. Russ J Plant Physiol 65, 203–210 (2018). https://doi.org/10.1134/S1021443718020164
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DOI: https://doi.org/10.1134/S1021443718020164