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Ethylene-dependent adjustment of metabolite profiles in Arabidopsis thaliana seedlings during gravitropic response

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Abstract

Metabolite profile adjustments under impact of ethylene synthesis inhibitors were studied in Arabidopsis thaliana (L.) Heynh. seedlings during reorientation of plants relative to the gravity vector (gravistimulation). Metabolite profiles were compared with Principal Component Analysis. We have shown that significant changes in metabolite profiles developed within 60 min of gravistimulation and were most pronounced in 2 mm root tips including the root cap, apical meristem and elongation zone. Gravistimulation resulted in the increased levels of valine, leucine, serine, γ-aminobutyric acid, nicotinic acid, and decreased levels of several monosaccharides, malate and oxalate. Treatment with ethylene synthesis inhibitor, aminoethoxyvinylglycine (10 μM), escaped the effect of gravistimulation on root tip metabolite profile. Metabolite profile adjustments revealed in this study suggest that ethylene may be involved into the regulation of Arabidopsis metabolome during the gravitropic response.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

AVG:

L-α-(2-Aminoethoxyvinyl)glycine

GC/MS:

gas chromatography–mass-spectrometry

PC:

principal component

PCA:

principal component analysis

SA:

salicylic acid

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Author information

Authors and Affiliations

  1. Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia

    G. A. Pozhvanov, N. S. Klimenko, T. E. Bilova & S. S. Medvedev

  2. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg, Russia

    N. S. Klimenko

  3. Komarov Botanical Institute of Russian Academy of Sciences, St. Petersburg, Russia

    A. L. Shavarda

Authors
  1. G. A. Pozhvanov
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  2. N. S. Klimenko
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  3. T. E. Bilova
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  4. A. L. Shavarda
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  5. S. S. Medvedev
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Corresponding author

Correspondence to G. A. Pozhvanov.

Additional information

Published in Russian in Fiziologiya Rastenii, 2017, Vol. 64, No. 6, pp. 446–460.

The article was translated by the authors.

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Pozhvanov, G.A., Klimenko, N.S., Bilova, T.E. et al. Ethylene-dependent adjustment of metabolite profiles in Arabidopsis thaliana seedlings during gravitropic response. Russ J Plant Physiol 64, 906–918 (2017). https://doi.org/10.1134/S1021443717050090

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  • DOI: https://doi.org/10.1134/S1021443717050090

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