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Ultrastructural changes and dynamic expressions of FAD7, Cu/Zn-SOD, and Mn-SOD in Neosinocalamus affinis under cold stress

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Abstract

As one of the fast-growing species, bamboo plays an important role in ecological stability and wood processing industry. However, low temperature limitation is the basic problem for the cultivation and introduction of bamboo. In this study, the symptoms of cold stress influence on the native bamboo (Neosinocalamus affinis (Rendle) Keng f.) and hybrid bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) were observed under transmission electron microscope, and the dynamic responses of FAD7, Cu/Zn-SOD, and Mn-SOD genes to cold stress were identified in bamboo by real-time quantitative RT-PCR. Observation by electron microscopy indicated that bamboo is one of the most chilling-sensitive species with severe ultrastructural injury induced by chilling, but the native bamboo (N. affinis) is more cold-tolerant compared with the hybrid bamboo. Results obtained by real-time quantitative RT-PCR analysis revealed that FAD7, Cu/Zn-SOD, and Mn-SOD were all cold-inducible genes in N. affinis. In addition, dynamic response patterns of N. affinis Cu/Zn-SOD and Mn-SOD under cold stress were similar. This work is a fundamental research of hardiness physiology of bamboo and may contribute to the breeding program on obtaining transgenic bamboo species.

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Abbreviations

CAT:

catalase

FAD7 :

gene of ω-3 fatty acid desaturase

POD:

peroxidase

qRT-PCR:

real-time quantitative RT-PCR

SOD:

superoxide dismutase

Cu/Zn-SOD :

gene of Cu, Zn-SOD

Mn-SOD :

gene of manganese-SOD

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Authors and Affiliations

  1. College of Landscape Architecture, Sichuan Agricultural University, Chengdu, 610000, China

    F. Zhang, X. Q. Zhu, Y. L. Guo, Q. B. Chen & M. Liu

  2. College of Forestry, Sichuan Agricultural University, Ya’an, 625014, China

    X. Q. Wan & T. T. Lin

  3. Institute of Rice Research, Sichuan Agricultural University, Chengdu, 610000, China

    P. Q. Liu

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  1. F. Zhang
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  2. X. Q. Zhu
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  3. Y. L. Guo
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  4. X. Q. Wan
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  5. T. T. Lin
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  6. Q. B. Chen
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  7. M. Liu
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Correspondence to F. Zhang.

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Zhang, F., Zhu, X.Q., Guo, Y.L. et al. Ultrastructural changes and dynamic expressions of FAD7, Cu/Zn-SOD, and Mn-SOD in Neosinocalamus affinis under cold stress. Russ J Plant Physiol 61, 760–767 (2014). https://doi.org/10.1134/S1021443714050173

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  • DOI: https://doi.org/10.1134/S1021443714050173

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