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Expression profiles of genes involved in starch synthesis in non-waxy and waxy wheat

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Abstract

Non-waxy and waxy types of wheat were used to study the expression profiles of genes involved in starch synthesis. During grain development, expression profiles and levels of AGPL and AGPS genes were similar to each other. SSI expression remained constant during the late grain development, while expression of SSII and SSIII was higher over the early to middle and middle grain development, and the GBSSI was actively expressed during the entire grain development. SBEIIa was higher expressed during early to middle stage, SBEIIb was active in middle and SBEI during middle to late grain development. During the entire grain development, expression levels of GBSSI and SSIII genes were higher in non-waxy type of wheat, while those of SBEI and SBEIIb were lower in the non-waxy type of wheat. Expression of all genes involved in starch synthesis was stage-specific and tissue-specific. In addition, the expression profiles of genes encoding starch synthase were in agreement with the activity changes of starch synthase during grain development.

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Abbreviations

AGPP:

adenosine diphosphate glucose pyrophosphorylase

AGPL :

gene encoding large subunit of AGPP

AGPS :

gene encoding small subunit of AGPP

DAA:

days after anthesis

DP:

degree of polymerization

SSS:

soluble starch synthase

SS :

gene encoding SSS

GBSSI:

granule-bound starch synthase I

GBSSI :

gene encoding GBSSI

SBE:

starch-branching enzyme

SBE :

gene encoding SBE

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Authors and Affiliations

  1. College of Agronomy, Northwest A&F University, No. 3 Taicheng Road, 712100, Yangling, Shaanxi, P.R. China

    Y. N. Cao & C. S. Wang

  2. Wheat Research Centre, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, P.R China

    W. G. Hu

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  1. Y. N. Cao
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  2. W. G. Hu
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  3. C. S. Wang
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Correspondence to C. S. Wang.

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Cao, Y.N., Hu, W.G. & Wang, C.S. Expression profiles of genes involved in starch synthesis in non-waxy and waxy wheat. Russ J Plant Physiol 59, 632–639 (2012). https://doi.org/10.1134/S1021443712050056

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