Skip to main content
SpringerLink
Log in

Effect of sodium nitroprusside on heat resistance of wheat coleoptiles: Dependence on the formation and scavenging of reactive oxygen species

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

The effect of nitric oxide donor sodium nitroprusside (SNP) on resistance of coleoptiles of 4-day-old etiolated seedlings of wheat (Triticum aestivum L., cv. Elegiya) to damaging heating (10 min at 43°C) and possible dependence of this effect on changes in the activities of enzymes producing and scavenging reactive oxygen species (ROS) were studied. Treatment of coleoptiles with 500 μM SNP considerably boosted generation of superoxide anion radical therein. This effect was substantially suppressed by blocker of calcium channels (lanthanum chloride), calmodulin antagonist (chlorpromazine), and inhibitor of NADPH-oxidase (imidazole) but not by peroxidase inhibitor (salicylhydroxamic acid). NO donor activated antioxidant enzymes (superoxide dismutase, catalase, and soluble peroxidase) and elevated heat resistance of wheat coleoptiles. NO scavenger methylene blue, antioxidant agent ionol, calcium antagonists, and NADPH-oxidase inhibitor imidazole substantially reduced the elevation of heat resistance of wheat coleoptiles induced by NO donor. It was concluded that SNP-induced heat resistance of coleoptiles depended on calcium and ROS, whose production is probably boosted by activation of NADPH-oxidase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

NBT:

nitro blue tetrazolium

SHAM:

salicylhy-droxamic acid

SNP:

sodium nitroprusside

SOD:

superoxide dismutase

References

  1. Wilson, I.D., Neill, S.J., and Hancock, J.T., Nitric Oxide Synthesis and Signaling in Plants, Plant Cell Environ., 2008, vol. 31, pp. 622–631.

    Article  PubMed  CAS  Google Scholar 

  2. Zhang, L., Zhou, S., Xuan, Y., Sun, M., and Zhao, L., Protective Effect of Nitric Oxide against Oxidative Damage in Arabidopsis Leaves under Ultraviolet-B Irradiation, J. Plant Biol., 2009, vol. 52, pp. 135–140.

    Article  Google Scholar 

  3. Zaninotto, F., Camera, S.L., Polverari, A., and Delledonne, M., Cross Talk Be-Tween Reactive Nitrogen and Oxygen Species during the Hypersensitive Disease Resistance Response, Plant Physiol., 2006, vol. 141, pp. 379–383.

    Article  PubMed  CAS  Google Scholar 

  4. Glyan’ko, A.K. and Vasil’eva, G.G., Activated Oxygen and Nitrogen Species in Legume-Rhizobium Symbiosis (Review), Prikl. Biokhim. Mikrobiol., 2010, vol. 46, pp. 21–28.

    Google Scholar 

  5. Krasylenko, Yu.A., Emets, A.I., and Blume, Ya.B., Functional Role of Nitric Oxide in Plants, Russ. J. Plant Physiol., 2010, vol. 57, pp. 451–461.

    Article  CAS  Google Scholar 

  6. Zhang, Y., Wang, L., Liu, Y., Zhang, Q., Wei, Q., and Zhang, W., Nitric Oxide Enhances Salt Tolerance in Maize Seedlings through Increasing Activities of Proton Pump and Na+/H+ Antiport in the Tonoplast, Planta, 2006, vol. 224, pp. 545–555.

    Article  PubMed  CAS  Google Scholar 

  7. Uchida, A., Jagendorf, A.T., Hibino, T., and Takabe, T., Effects of Hydrogen Peroxide and Nitric Oxide on Both Salt and Heat Stress Tolerance in Rice, Plant Sci., 2002, vol. 163, pp. 515–523.

    Article  CAS  Google Scholar 

  8. Hossain, K.K., Itoh, R.D., Ioshimura, G., Tokuda, G., Oku, H., Cohen, M.F., and Yamasaki, H., Effects of Nitric Oxide Scavengers on Thermoinhibition of Seed Germination in Arabidopsis thaliana, Russ. J. Plant Physiol., 2010, vol. 57, pp. 222–232.

    Article  CAS  Google Scholar 

  9. Tewari, R.K., Hahn, E.J., and Paek, K.Y., Function of Nitric Oxide and Superoxide Anion in the Adventitious Root Development and Antioxidant Defence in Panax ginseng, Plant Cell Rep., 2008, vol. 27, pp. 563–573.

    CAS  Google Scholar 

  10. Viktorova, L.V., Maksyutova, N.N., Trifonova, T.V., and Andrianov, V.V., Hydrogen Peroxide and Nitric Oxide Generation Induced by Nitrate and Nitrite Operation into the Apoplast of Wheat Leaves, Biochimiya, 2010, vol. 75, pp. 117–124.

    Google Scholar 

  11. Lu, D., Zhang, X., Jiang, J., An, G.Y., Zhang, L.R., and Song C.P. NO May Function in the Downstream of H2O2 in ABA-Induced Stomatal Closure in Vicia faba L., J. Plant Physiol. Mol. Biol., 2005, vol. 31, pp. 62–70.

    Google Scholar 

  12. Zhang, A., Jiang, M., Zhang, J., Ding, H., Xu, S., Hu, X., and Tan, M., Nitric Oxide Induced by Hydrogen Peroxide Mediates Abscisic Acid-Induced Activation of the Mitogen-Activated Protein Kinase Cascade Involved in Antioxidant Defense in Maize Leaves, New Phytol., 2007, vol. 175, pp. 36–50.

    Article  PubMed  CAS  Google Scholar 

  13. Lamotte, O., Gould, K., Lecourieux, D., Sequeira-Legrand, A., Lebrun-Garcia, A., Durner, J., Pugin, A., and Wendehenne, D., Analysis of Nitric Oxide Signalling Functions in Tobacco Cells Challenged by the Elicitor Cryptogein, Plant Physiol., 2004, vol. 135, pp. 516–529.

    Article  PubMed  CAS  Google Scholar 

  14. Courtois, C., Besson, A., Dehan, J., Bourque, S., Dobrowolska, G., Pugin, A., and Wendehenne, D., Nitric Oxide Signalling in Plants: Interplays with Ca2+ and Protein Kinases, J. Exp. Bot., 2008, vol. 59, pp. 155–163.

    Article  PubMed  CAS  Google Scholar 

  15. Kolupaev, Yu.E., Akinina, G.E., and Mokrousov, A.V., Induction of Heat Tolerance in Wheat Coleoptiles by Calcium Ions and Its Relation to Oxidative Stress, Russ. J. Plant Physiol., 2005, vol. 52, pp. 199–204.

    Article  CAS  Google Scholar 

  16. Kolupaev, Yu.E., Karpets, Yu.V., Yastreb, T.O., and Musatenko, L.I., Peroxidase and Superoxide Dismutase Contribute to the Enhancement of Activated Oxygen Generation in Wheat Coleoptiles Induced by Salicylic Acid, Fiziol. Biokh. Kul’t. Rast., 2010, vol. 42, pp. 210–217.

    CAS  Google Scholar 

  17. Shorning, B.Yu., Smirnova, E.G., Yaguzhinskii, L.S., and Vanyushin, B.F., Necessity of Superoxide Generation for Wheat Etiolated Seedling Development, Biokhimiya, 2000, vol. 65, pp. 1612–1618.

    Google Scholar 

  18. Zherelova, O.M. and Chailakhyan, L.M., Ca2+-Channels in Plant Cells and Their Regulation, Usp. Sovrem. Biol., 1994, vol. 114, pp. 608–619.

    CAS  Google Scholar 

  19. Hung, K.T., Hsu, Y.T., and Kao, C.H., Hydrogen Peroxide Is Involved in Methyl Jasmonate-Induced Senescence of Rice Leaves, Physiol. Plant., 2006, vol. 127, pp. 293–303.

    Article  CAS  Google Scholar 

  20. Mori, I.C., Pinontoan, R., Kawano, T., and Muto, S., Involvement of Superoxide Generation in Salicylic Acid-Induced Stomatal Closure in Vicia faba, Plant Cell Physiol., 2001, vol. 42, pp. 1383–1388.

    Article  CAS  Google Scholar 

  21. Ridge, I. and Osborne, D.J., Hydroxyproline and Peroxidases in Cell Walls of Pisum sativum: Regulation by Ethylene, J. Exp. Bot., 1970, vol. 21, pp. 843–856.

    Article  CAS  Google Scholar 

  22. Foyer, C.H. and Noctor, G., Redox Regulation in Photosynthetic Organisms: Signaling, Acclimation, and Practical Implications, Antioxid. Redox Signal., 2009, vol. 11, pp. 861–906.

    Article  PubMed  CAS  Google Scholar 

  23. Asada, K., Ascorbate Peroxidase — a Hydrogen Peroxide-Scavenging Enzymes in Plants, Physiol. Plant., 1992, vol. 85, pp. 235–241.

    Article  CAS  Google Scholar 

  24. Wong, H.L., Pinontoan, R., Hayashi, K., Tabata, R., Yaeno, T., Hasegawa, K., Kojima, C., Yoshioka, H., Iba, K., Kawasaki, T., and Shimamoto, K., Regulation of Rice NADPH-Oxidase by Rac GTPase to Its N-Terminal Extension, Plant Cell, 2007, vol. 19, pp. 4022–4034.

    Article  PubMed  CAS  Google Scholar 

  25. Herbette, S., Lenne, C., de Labrouhe, D.T., Drevet, J.R., and Roeckel-Drevet, P., Transcripts of Sunflower Antioxidant Scavengers of the SOD and GPX Families Accumulate Differentially in Response to Downy Mildew Infection, Phytohormones, Reactive Oxygen Species, Nitric Oxide, Protein Kinase and Phosphatase Inhibitors, Physiol. Plant., 2003, vol. 119, pp. 418–428.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dokuchaev National Agrarian University, Kommunist-1, Khar’kov, 62483, Ukraine

    Yu. V. Karpets, Yu. E. Kolupaev & T. O. Yastreb

Authors
  1. Yu. V. Karpets
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu. E. Kolupaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. O. Yastreb
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu. V. Karpets.

Additional information

Original Russian Text ©Yu.V. Karpets, Yu.E. Kolupaev, T.O. Yastreb, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 6, pp. 883–890.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karpets, Y.V., Kolupaev, Y.E. & Yastreb, T.O. Effect of sodium nitroprusside on heat resistance of wheat coleoptiles: Dependence on the formation and scavenging of reactive oxygen species. Russ J Plant Physiol 58, 1027–1033 (2011). https://doi.org/10.1134/S1021443711060094

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443711060094

Keywords

Search

Navigation