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Responses of chlorophyll b-less chlorina 3613 mutant of barley to a prolonged decrease in illuminance: 2. Dynamics of carotenoids in leaf chloroplasts

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Abstract

Barley (Hordeum vulgare L.) mutant chlorina 3613 is notable for a lack of chlorophyll b (Chl b), low content of chlorophyll a (Chl a) and carotenoids in the chloroplasts, as well as reduction in the majority of components of LHCI and LHCII. Incompletely developed photosynthetic machinery of chlorina 3613 results in suppressed growth, lower biomass, and the declined rate of photosynthesis (as compared with the wild-type cv. Donaria). The lack of Chl b and greater part of peripheral antenna suggests that this mutant will have difficulties during acclimation to long-term shading because the light-harvesting role of Chl b-containing antenna becomes more important under the shortage of light. Earlier, our experiments with the mature chlorina 3613 plants shaded for one week at PAR photon flux density of 60 and 40% of that in full sunlight showed a stimulating effect of shading on growth, biomass accumulation, and Chl a synthesis in chlorina 3613 when biosynthesis of Chl b did not occur [1]. In this work, we investigated in more detail the changes in the content of carotenoids in chlorina 3613. We found that in Donaria at both investigated levels of illumination (60 and 40% of full sunlight) and in chlorina 3613 at 60% illumination, moderate reversible changes typical of shade-enduring plants occur in the content of carotenoids. In chlorina 3613 at 40% illumination, the content of β-carotene increased considerably (by 3 times) with simultaneous accumulation of Chl a. When full illumination was restored, the content of β-carotene decreased and remained on the level, which exceeded its initial content in the plants without shading by 38%; this level, was maintained by the end of vegetation. The changes in the contents of β-carotene and Chl a in chlorina 3613 were not accompanied by any accumulation of xanthophylls or changes in the relative content of active violaxanthin. The obtained results suggest that a long-term shading of the leaves of mature chlorina 3613 plants induced the formation of certain components of photosynthetic apparatus: reactive centers and core parts of photosystems’ antennae as well as proteins CP26 and CP29 and in this way contributed to partial restoration of photosynthetic activity and production process in the mutant lacking Chl b.

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Abbreviations

Chl a :

chlorophyll a

Chl b :

chlorophyll b

LHC:

light-harvesting complex

VC:

violaxanthin cycle

References

  1. Tyutereva, E.V. and Voitsekhovskaya, O.V., Responses of Chlorophyll b-Free chlorina 3613 Barley Mutant to a Prolonged Decrease in Illuminance: 1. Dynamics of Chlorophyll Content, Growth, and Productivity, Russ. J. Plant Physiol., 2011, vol. 58, pp. 1–8.

    Article  CAS  Google Scholar 

  2. Dekker, J.P. and Boekema, E.J., Supramolecular Organization of Thylakoid Membrane Proteins in Green Plants, Biochim. Biophys. Acta, 2005, vol. 1706, pp. 12–39.

    Article  PubMed  CAS  Google Scholar 

  3. Jensen, P.E., Bassi, R., Boekema, E.J., Dekker, J.P., Jansson, S., Leister, D., Robinson, C., and Vibe, Scheller, H., Structure, Function and Regulation of Plant Photosystem I, Biochim. Biophys. Acta, 2007, vol. 1767, pp. 335–352.

    Article  PubMed  CAS  Google Scholar 

  4. Melis, A., Dynamics of Photosynthetic Membrane Composition, Biochim. Biophys. Acta, 1991, vol. 1058, pp. 87–106.

    Article  CAS  Google Scholar 

  5. Ruban, A.V., Berera, R., Ilioaia, C., van Stokkum, I.H.M., Kennis, J.T.M., Pascal, A.A., van Amerongen, H., Robert, B., Horton, P., and van Grondelle, R., Identification of a Mechanism of Photoprotective Energy Dissipation in Higher Plants, Nature, 2007, vol. 450, pp. 575–578.

    Article  PubMed  CAS  Google Scholar 

  6. Apel, P., Photosynthesemessungen an Chlorophyllmutanten von Gerste (Lichtkurven, “Light-Atmung”, Starklichtemfindlichkeit), Stud. Biophys., 1967, vol. 5, pp. 105–110.

    Google Scholar 

  7. Leverenz, J.W., Öquist, G., and Winglse, G., Photosynthesis and Photoinhibition in Leaves of Chlorophyll b-Less Barley in Relation to Absorbed Light, Physiol. Plant., 1992, vol. 85, pp. 495–502.

    Article  CAS  Google Scholar 

  8. Machold, O., Meister, A., Sagromsky, H., Hoyer-Hansen, G., and Wettstein, D., Composition of Photosynthetic Membranes of Wild-Type Barley and Chlorophyll b-Less Mutants, Photosynthetica, 1977, vol. 11, pp. 240–245.

    Google Scholar 

  9. Armond, P.A., Staehelin, L.A., and Arntzen, C.J., Spatial Relationship of Photosystem I, Photosystem II and Light Harvesting Complex II in Chloroplast Membranes, J. Cell Biol., 1977, vol. 73, pp. 400–418.

    Article  PubMed  CAS  Google Scholar 

  10. Falbel, T.G., Mehl, J.B., and Staehelin, L.A., Severity of Mutant Phenotype in a Series of Chlorophyll-Deficient Wheat Mutants Depends on Light Intensity and the Severity of the Block in Chlorophyll Synthesis, Plant Physiol., 1996, vol. 112, pp. 821–832.

    Article  PubMed  CAS  Google Scholar 

  11. Tyutereva, E.V., Ivanova, A.N., and Voitsekhovskaya, O.V., Structural and Functional Reorganization of the Photosynthetic Apparatus in Barley Mutant chlorina 3613 by Changing in the Light Level, Fundamental’nye i prikladnye problemy botaniki v nachale XXI veka, Ch. 6, Mater. dokl. XII S“ezda Russkogo bot. ob-va (Fundamental and Applied Problems in Botany at the Beginning of the XXI Century, Chapter 6), Petrozavodsk, 2008, pp. 132–134.

  12. Sapozhnikov, D.I. and Kornyushenko, G.A., Heterogeneity of Violaxanthin in Pea Leaves, Sov. Plant Physiol., 1969, vol. 16, pp. 1038–1041.

    CAS  Google Scholar 

  13. Ruban, A.V., Young, A.J., and Horton, P., Dynamic Properties of the Minor Chlorophyll a/b Binding Proteins of Photosystem II, an In Vitro Model for Photoprotective Energy Dissipation in the Photosynthetic Membrane of Green Plants, Biochemistry, 1996, vol. 35, pp. 674–678.

    Article  PubMed  CAS  Google Scholar 

  14. Bassi, R., Pineau, B., Dainese, P., and Marquardt, J., Carotenoid Binding Proteins of Photosystem II, Eur. J. Biochem., 1993, vol. 212, pp. 297–303.

    Article  PubMed  CAS  Google Scholar 

  15. Härtel, H., Lokstein, H., Grimm, B., and Rank, B., Kinetic Studies on the Xanthophyll Cycle in Barley Leaves (Influence of Antenna Size and Relations to Nonphotochemical Chlorophyll Fluorescence Quenching), Plant Physiol., 1996, vol. 110, pp. 471–482.

    PubMed  Google Scholar 

  16. Lichtenthaler, H.K. and Wellburn, A.R., Determination of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents, Biochem. Soc. Trans., 1983, vol. 11, pp. 591–592.

    CAS  Google Scholar 

  17. Anderson, J.M., Photoregulation of the Composition, Function and Structure of Thylakoid Membranes, Annu. Rev. Plant Physiol., 1986, vol. 37, pp. 93–136.

    Article  CAS  Google Scholar 

  18. Leong, T.-Y. and Anderson, J.M., Light-Quality and Irradiance Adaptation of the Composition and Function of Pea Thylakoid Membranes, Biochim. Biophys. Acta, 1986, vol. 850, pp. 57–63.

    Article  CAS  Google Scholar 

  19. Lichtenthaler, H.K. and Meier, D., Regulation of Chloroplast Photomorphogenesis by Light Intensity and Light Quality, Chloroplast Biogenesis, Ellis, R.J., Ed., Cambridge: Cambridge Univ. Press, 1984, pp. 261–281.

    Google Scholar 

  20. Akoyunoglou, G. and Argyroudi-Akoyunoglou, J.H., Post-Translation Regulation of Chloroplast Differentiation, Regulation of Chloroplast Differentiation, Plant Biology, vol. 2, Akoyunoglou, G. and Senger, H., Eds., New York: Alan R. Liss, 1986, pp. 571–582.

    Google Scholar 

  21. Bellmare, G., Bartlett, S.G., and Chua, N.H., Biosynthesis of Chlorophyll a/b-Binding Polypeptides in Wild Type and the chlorina f2 Mutant of Barley, J. Biol. Chem., 1982, vol. 257, pp. 7762–7767.

    Google Scholar 

  22. Tanaka, R. and Tanaka, A., Chlorophyll b Is Not Just an Accessory Pigment but a Regulator of the Photosynthetic Antenna, Porphyrins, 2000, vol. 9, pp. 240–245.

    Google Scholar 

  23. Hirashima, M., Satoh, S., Tanaka, R., and Tanaka, A., Pigment Shuffling in Antenna Systems Achieved by Expressing Prokaryotic Chlorophyllide a Oxygenase in Arabidopsis, J. Biol. Chem., 2006, vol. 281, pp. 15385–15393.

    Article  PubMed  CAS  Google Scholar 

  24. Williams, N.D., Joppa, L., Duysen, M.E., and Freeman, T.P., Inheritance of Three Chlorophyll-Deficient Mutants of Common Wheat, Crop Sci., 1985, vol. 25, pp. 1023–1025.

    Article  Google Scholar 

  25. Allen, K.D., Duysen, M.E., and Staehelin, L.A., Biogenesis of Thylakoid Membranes Is Controlled by Light Intensity in the Conditional Chlorophyll b-Deficient CD3 Mutant of Wheat, J. Cell Biol., 1988, vol. 107, pp. 907–919.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Ecological Physiology, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, St. Petersburg, 197376, Russia

    E. V. Tyutereva & O. V. Voitsekhovskaja

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  1. E. V. Tyutereva
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  2. O. V. Voitsekhovskaja
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Correspondence to O. V. Voitsekhovskaja.

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Original Russian Text © E.V. Tyutereva, O.V. Voitsekhovskaja, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 2, pp. 186–194.

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Tyutereva, E.V., Voitsekhovskaja, O.V. Responses of chlorophyll b-less chlorina 3613 mutant of barley to a prolonged decrease in illuminance: 2. Dynamics of carotenoids in leaf chloroplasts. Russ J Plant Physiol 58, 218–225 (2011). https://doi.org/10.1134/S1021443711010237

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