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Vacuolar symplast formation is due to highly permeable gap junctions between the tonoplast and endoplasmic reticulum membrane

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Abstract

An NMR method with a pulsed magnetic field gradient was applied to study changes in water permeability of the vacuolar symplast in maize (Zea mays L.) seedling roots treated with various inhibitors of cell metabolism. The results were qualitatively analogous to literature data on conductivity changes of intercellular gap junctions in animal cells exposed to similar treatments. Electron microscopy examination of root cells provided evidence for the existence of membrane contacts between the endoplasmic reticulum and the tonoplast. It is supposed that vacuoles of neighboring plant cells are interconnected through highly dynamical gap junctions between the tonoplast and the endoplasmic reticulum membrane.

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Abbreviations

BDM:

2,3-butanedione monoxime

DNP:

2,4-dintrophenol

ER:

endoplasmic reticulum

PMFG NMR:

pulsed magnetic field gradient NMR method

SDC:

self-diffusion coefficient

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, a/ya 30, Kazan, Tatarstan, 420111, Russia

    G. A. Velikanov, L. P. Belova & V. Yu. Levanov

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  1. G. A. Velikanov
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  2. L. P. Belova
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  3. V. Yu. Levanov
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Correspondence to G. A. Velikanov.

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Original Russian Text © G.A. Velikanov, L.P. Belova, V.Yu. Levanov, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 6, pp. 921–930.

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Velikanov, G.A., Belova, L.P. & Levanov, V.Y. Vacuolar symplast formation is due to highly permeable gap junctions between the tonoplast and endoplasmic reticulum membrane. Russ J Plant Physiol 55, 834–842 (2008). https://doi.org/10.1134/S1021443708060149

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  • DOI: https://doi.org/10.1134/S1021443708060149

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