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Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants

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Abstract

Promoters of carnation etched ring virus (CERV) and dahlia mosaic virus (DMV) were cloned into binary vectors pCambia 1304, pCambia 1281Z, and pCambia 1291Z with reporter GFP and GUS genes. Activities of these promoters in tobacco protoplasts and transgenic plants were determined using these constructs. Histochemical GUS analysis demonstrated the absence of tissue-specificity in transgenic plants transformed with these promoters. The quantitative analysis of these promoter activities in transgenic tobacco plants, using 4-methylumbelliferone as a substrate, showed that 35S CaMV, CERV, and DMV promoters displayed approximately similar activities in transgenic tobacco plants.

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Abbreviations

BA:

benzyladenine

CaMV:

cauliflower mosaic virus

CERV:

carnation etched ring virus

DMV:

dahlia mosaic virus

GFP:

green fluorescent protein

GUS:

β-glucuronidase

MS:

Murashige and Skoog nutrient medium

X-Gluc:

5-bromo-4-chloro-3-indoxyl-β-D-glucuronide

4-MU:

4-methylumbelliferone

4-MUG:

4-methylumbelliferone-β-D-glucuronide

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa, 450054, Russia

    B. R. Kuluev, A. V. Knyazev & A. V. Chemeris

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  1. B. R. Kuluev
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  2. A. V. Knyazev
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  3. A. V. Chemeris
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Correspondence to B. R. Kuluev.

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Original Russian Text © B.R. Kuluev, A.V. Knyazev, A.V. Chemeris, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 5, pp. 763–770.

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Kuluev, B.R., Knyazev, A.V. & Chemeris, A.V. Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants. Russ J Plant Physiol 55, 687–693 (2008). https://doi.org/10.1134/S1021443708050130

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