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Effect of Multipotent Mesenchymal Stromal Cells on Functional Activity of Monocyte-Derived Macrophages under Short-Term Hypoxic Stress in Vitro

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Abstract

The study focused on the interaction between mesenchymal stromal cells (MSCs) and monocyte-derived macrophages (MN/MPs). The interactions play an important role in MSC in vivo indirect therapeutic effects and anti-inflammatory and immunomodulatory properties. To assess the MSC effect on MN/MP functional activity, MSCs and MN/MPs were co-cultured in standard conditions (20% O2) for 6 days. Then part of MN/MP and MN/MP + MSC cultures was exposed to short-term hypoxic stress (1% O2, 24 h), while the other part was further cultured at 20% O2. In both standard conditions and hypoxia, MSCs exerted stromal activity and increased the MN/MP viability by decreasing the MN/MP portion undergoing early apoptosis. A paracrine interaction stimulated phagocytic activity of MN/MPs without affecting activity of the lysosomal compartment at 20% O2. Hypoxic stress decreased the MSC-induced phagocytic potential and lysosomal activity. An anti-inflammatory phenotype developed in MN/MPs upon their interaction with MSCs, and MN/MPs consequently better utilized their phagocytic potential in the presence of MSCs in spite of oxygen deprivation.

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Funding

This work was supported by the Russian Foundation for Basic Research (project no. 17-04-00942).

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    O. Yu. Alekseeva, P. I. Bobyleva & E. R. Andreeva

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  1. O. Yu. Alekseeva
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  2. P. I. Bobyleva
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  3. E. R. Andreeva
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Correspondence to O. Yu. Alekseeva.

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Conflict of interests. The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human subjects performed by any of the authors.

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Translated by T. Tkacheva

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Alekseeva, O.Y., Bobyleva, P.I. & Andreeva, E.R. Effect of Multipotent Mesenchymal Stromal Cells on Functional Activity of Monocyte-Derived Macrophages under Short-Term Hypoxic Stress in Vitro. Hum Physiol 48, 899–905 (2022). https://doi.org/10.1134/S0362119722070155

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