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The Effect of Long-term Space Flights on Human Urine Proteins Functionally Related to Endothelium

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Abstract

It is known that long-term space flights lead to dysregulation of the cardiovascular system, and the endothelium is the most important functional element of such dysregulation. In order to find the signs of endothelial dysfunction in cosmonauts who have been in long-term space flights, we collected urine samples from 21 cosmonauts before the flight and on the first and seventh days after landing. The urine samples were investigated by chromatography–mass spectrometry analysis. Proteins were identified using the MaxQuant software and the SwissProt database. The software package Perseus was used for semi-quantitative analysis. The reconstruction of associative molecular networks was performed using the ANDSystem software. We identified 200 different proteins in urine samples of 21 Russian cosmonauts. The ANDSystem software made it possible to determine seven processes related to endothelium functioning. These processes had direct relations to 17 urine proteins, which were functionally associated with the endothelium. At the same time, eight proteins (such as serotransferrin, prostate-specific antigen, fibrinogen gamma chain, UFO tyrosine kinase receptor, aminopeptidase N, vascular cell adhesion molecule 1, osteopontin, and syndecan-4) were significantly changed (p < 0.01) at different points of the recovery period (the first and seventh days). Thus, we performed the first study of the urine protein composition in cosmonauts for the evaluation of signs of endothelial dysfunction after space flight using proteomics methods.

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Authors and Affiliations

  1. Institute for Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia

    L. Kh. Pastushkova, D. N. Kashirina, A. S. Kononikhin, A. G. Brzhozovsky & I. M. Larina

  2. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    V. A. Ivanisenko & E. S. Tiys

  3. Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, 117303, Russia

    A. S. Kononikhin & E. N. Nikolaev

  4. Center for Clinical Studies, Angers University Clinic, Angers, 49035, France

    A. M. Novosyolova & M. -A. Custaud

  5. Tal’roze Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    A. S. Kononikhin & E. N. Nikolaev

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  1. L. Kh. Pastushkova
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  2. D. N. Kashirina
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  3. A. S. Kononikhin
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  7. A. M. Novosyolova
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  9. E. N. Nikolaev
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Correspondence to L. Kh. Pastushkova.

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Original Russian Text © L.Kh. Pastushkova, D.N. Kashirina, A.S. Kononikhin, A.G. Brzhozovsky, V.A. Ivanisenko, E.S. Tiys, A.M. Novosyolova, M.-A. Custaud, E.N. Nikolaev, I.M. Larina, 2018, published in Fiziologiya Cheloveka, 2018, Vol. 44, No. 1, pp. 72–81.

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Pastushkova, L.K., Kashirina, D.N., Kononikhin, A.S. et al. The Effect of Long-term Space Flights on Human Urine Proteins Functionally Related to Endothelium. Hum Physiol 44, 60–67 (2018). https://doi.org/10.1134/S0362119718010139

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