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Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity

  • Biophysical Chemistry
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The aim of this study was to examine in vitro chelator’s ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO4 concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC50 values, 1.15 × 10−4 and 1.71 × 10−3 mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO4 concentration for half-maximal inhibition (IC50), by decreasing Cu2+ concentrations, available to form inactive CuATP2− complex.

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Authors and Affiliations

  1. Laboratory for Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, P.O. Box 522, 11001, Belgrade, Serbia

    M. Milošević, S. Petrović, I. Stanojević, D. Drakulić, N. Veličković & A. Horvat

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  1. M. Milošević
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  2. S. Petrović
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  3. I. Stanojević
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  4. D. Drakulić
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  5. N. Veličković
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  6. A. Horvat
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Correspondence to M. Milošević.

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Milošević, M., Petrović, S., Stanojević, I. et al. Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity. Russ. J. Phys. Chem. 83, 1592–1595 (2009). https://doi.org/10.1134/S0036024409090313

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  • DOI: https://doi.org/10.1134/S0036024409090313

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