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Can the Ovum Genome of Tetraploid Sturgeon Species (Acipenseridae) Exhibit the Functional Properties of a Diploid Genome?

  • STURGEONS (ACIPENSERIDAE)
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Abstract

Viable prelarvae were obtained for the first time as a result of insemination of eggs of the kaluga Acipenser dauricus with UV-inactivated sperm of the sterlet A. ruthenus. These normally developed prelarvae without any signs of the haploid syndrome carried two maternal alleles at each examined microsatellite locus; they did not have sterlet paternal alleles. The viability of haploid gynogenetic prelarvae is explained by the low level of diploidization of the kaluga genome, which is confirmed by the presence of multivalent configurations (mainly quadrivalents) in prophase I of meiosis in kaluga (with ~260 chromosomes). The results correspond to the extremely low rate of molecular and chromosomal evolution noted for all sturgeons.

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ACKNOWLEDGMENTS

We are very grateful to D. Amvrosov and E. Rachek who provided us with materials from LRFBS for our experimental crosses and genetic analyzes, as well as for their help in organizing and conducting our research at LRFBS. The authors also thank the anonymous reviewers for their valuable comments and suggestions, which undoubtedly improved the manuscript.

Funding

Experimental crosses in LRFBS, as well as microsatellite and synaptonemal complex analyses were conducted in 2017 and 2018 with partial financial support of the Russian Fund of Basic Researches; further scientific investigations of Ekaterina Vasil’eva were carried within a State Project of Moscow State University no. 121032300105-0.

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  1. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Moscow, Russia

    V. P. Vasil’ev & S. A. Simanovsky

  2. Russian Federal Research Institute of Fisheries and Oceanography, Moscow, Russia

    A. E. Barmintseva

  3. Lomonosov Moscow State University, Biological Department, Zoological Museum, Moscow, Russia

    E. D. Vasil’eva

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Vasil’ev, V.P., Simanovsky, S.A., Barmintseva, A.E. et al. Can the Ovum Genome of Tetraploid Sturgeon Species (Acipenseridae) Exhibit the Functional Properties of a Diploid Genome?. J. Ichthyol. 62, 1430–1438 (2022). https://doi.org/10.1134/S0032945222060303

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