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The role of the glycoprotein gp130 in the serotonin mediator system in the mouse brain

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Abstract

Glycoprotein gp130 is involved in signal transduction from the receptors of such important cytokines as interleukin-6 (IL-6), leukemia inhibitory factor, and ciliary neurotrophic factor, which play a critical role in immunity, inflammation, and neurogenesis. Both IL-6 and the brain neurotransmitter serotonin are involved in the mechanism of depression. The aim of this work was to investigate the role of gp130 in regulating the gene expression of the tryptophan hydroxylase 2 (TPH2), the key enzyme of the serotonin synthesis, as well as of the 5-HT transporter and the 5-HT1A and 5-HT2A receptors. The study was carried out on adult male mice of the congenic strains AKR and AKR.CBA-D13Mit76; the latter was created by transferring a gp130-containing fragment of chromosome 13 from the CBA/Lac strain into the AKR/J genome. The expression of 5-HT1A and 5-HT2A receptor genes in the hippocampus and midbrain and of the TPH2 gene in the midbrain was decreased in AKR.CBA-D13Mit76 mice in comparison to AKR mice. Activation of nonspecific immunity by administration of a bacterial endotoxin lipopolysaccharide did not affect the gene expression in AKR mice but increased the 5-HT2A receptor expression in the midbrain and decreased the 5-HT1A receptor expression in the cortex in AKR.CBA-D13Mit76 mice. These results suggest that gp130 is involved in the regulation of TPH2, 5-HT1A and 5-HT2A receptor genes and is associated with the genetically determined sensitivity to lipopolysaccharides.

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Abbreviations

RT:

reverse transcription

PCR:

polymerase chain reaction

5-HT:

serotonin

5-HT1A receptor:

subtype 1A serotonin receptor

5-HT2A receptor:

subtype 2A serotonin receptor

TPH:

tryptophan hydroxylase

LPS:

lipopolysaccharide

ip:

intraperitoneally

LIF:

leukemia inhibitory factor

CNTF:

ciliary neurotrophic factor

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, Novosibirsk, 630090, Russia

    A. V. Kulikov, V. S. Naumenko, A. S. Tsybko, N. A. Sinyakova, D. V. Bazovkina & N. K. Popova

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  1. A. V. Kulikov
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  2. V. S. Naumenko
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  3. A. S. Tsybko
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  4. N. A. Sinyakova
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  5. D. V. Bazovkina
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Correspondence to A. V. Kulikov.

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Original Russian Text © A.V. Kulikov, V.S. Naumenko, A.S. Tsybko, N.A. Sinyakova, D.V. Bazovkina, N.K. Popova, 2010, published in Molekulyarnaya Biologiya, 2010, Vol. 44, No. 5, pp. 904–910.

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Kulikov, A.V., Naumenko, V.S., Tsybko, A.S. et al. The role of the glycoprotein gp130 in the serotonin mediator system in the mouse brain. Mol Biol 44, 801–806 (2010). https://doi.org/10.1134/S0026893310050171

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