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Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene

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Abstract

Mouse embryonic fibroblasts (MEF) with point mutation in somatic cytochrome C gene were generated and characterized. It was shown that the substitution of lysine for tryptophan in position 72 (K72W) decreased the proapoptotic functions of cytochrome C in response to staurosporin treatment without disrupting its respiratory functions. The presence of this mutation did not affect the pattern of cytochrome C gene expression or its localization inside the cell. These cell lines therefore represent an interesting model for the study of apoptotic signaling and physiological functions of cytochrome C.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    I. A. Mufazalov, D. N. Penkov, A. A. Kruglov, D. V. Kuprash & S. A. Nedospasov

  2. Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119899, Russia

    I. A. Mufazalov, A. A. Kruglov & V. P. Skulachev

  3. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Academy of Sciences, Moscow, 119899, Russia

    B. V. Chernyak, O. Y. Pletjushkina, M. Y. Vyssokikh, V. P. Skulachev & S. A. Nedospasov

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia

    R. V. Chertkova, M. P. Kirpichnikov & D. A. Dolgikh

Authors
  1. I. A. Mufazalov
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  2. D. N. Penkov
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  3. B. V. Chernyak
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  4. O. Y. Pletjushkina
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  5. M. Y. Vyssokikh
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  6. R. V. Chertkova
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  7. M. P. Kirpichnikov
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  8. D. A. Dolgikh
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  9. A. A. Kruglov
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  10. D. V. Kuprash
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  11. V. P. Skulachev
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  12. S. A. Nedospasov
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Corresponding author

Correspondence to S. A. Nedospasov.

Additional information

Original Russian Text © I.A. Mufazalov, D.N. Penkov, B.V. Chernyak, O.Y. Pletjushkina, M.Y. Vyssokikh, R.V. Chertkova, M.P. Kirpichnikov, D.A. Dolgikh, A.A. Kruglov, D.V. Kuprash, V.P. Skulachev, S.A. Nedospasov, 2009, published in Molekulyarnaya Biologiya, 2009, Vol. 43, No. 4, pp. 648–656.

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Mufazalov, I.A., Penkov, D.N., Chernyak, B.V. et al. Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in somatic cytochrome C gene. Mol Biol 43, 596–603 (2009). https://doi.org/10.1134/S0026893309040116

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  • DOI: https://doi.org/10.1134/S0026893309040116

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