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Metagenomic Analysis of Bottom Sediments of the Karst Meromictic Lake Black Kichier Revealed Abundant Unculturable Thermoplasmatota

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Abstract

Investigations into the microbial community of bottom sediments in the karst Lake Black Kichier were conducted. These sediments exhibited elevated levels of sulfide, dissolved methane, and organic matter. Direct radiotracer experiments revealed substantial rates of microbial processes involved in the decomposition of organic matter. Uncultivated archaea belonging to the phylum Thermoplasmatota were identified within the microbial community. Metagenomic analysis unveiled representatives from five orders: Methanomassiliicoccales, Thermoprofundales (formerly known as Marine Benthic Group D and DHVEG-1), DTX01, SG8-5, and Candidatus Gimiplasmatales (formerly UBA10834). These archaea were previously believed to occur exclusively in deep marine sediments characterized by extreme organic matter scarcity. This discovery reshapes our understanding of the role played by Thermoplasmatota archaea, spanning five orders, in the degradation segment of the carbon cycle.

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ACKNOWLEDGMENTS

The authors are grateful to the staff of the Kichiyer Sanatorium (Republic of Mari El) for organizational assistance in setting up a temporary laboratory.

Funding

Field works, sample processing, and molecular-biological data analysis performed by researchers of the Federal Research Center of Biotechnology of the Russian Academy of Sciences were supported by the Russian Science Foundation, project no. 22-14-00038. Radiotracer experiments were performed by I.I. Rusanov in the framework of State Assignment of the Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Skryabin Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 117312, Moscow, Russia

    V. V. Kadnikov & A. V. Beletskii

  2. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312, Moscow, Russia

    A. S. Savvichev, I. I. Rusanov & N. V. Pimenov

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  1. V. V. Kadnikov
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  2. A. S. Savvichev
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  3. I. I. Rusanov
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  4. A. V. Beletskii
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  5. N. V. Pimenov
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Correspondence to V. V. Kadnikov.

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Translated by E. Makeeva

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Kadnikov, V.V., Savvichev, A.S., Rusanov, I.I. et al. Metagenomic Analysis of Bottom Sediments of the Karst Meromictic Lake Black Kichier Revealed Abundant Unculturable Thermoplasmatota. Microbiology 93, 128–133 (2024). https://doi.org/10.1134/S0026261723604359

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  • DOI: https://doi.org/10.1134/S0026261723604359

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