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Endophytic Fungi Pezicula radicicola in the Root Nodules of Actinorhizal Plants

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Abstract—

Based on analysis of the nucleotide sequences of the ITS regions, LSU gene fragments of rDNA, and cultural and morphological characteristics, species affiliation of six strains of mycelial fungi isolated from root nodules of actinorhizal plants in the subtropical zone (Corriaria myrtifolia, Myrica cerifera, and Eleagnus pungeus) was determined. The micromycetes studied were identified as Pezicula radicicola. Members of this species have not previously been revealed in the root nodules of actinorhizal plants. The possible functions and ecological role of Pezicula radicicola as mycosymbionts of actinorhizal plants are discussed.

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REFERENCES

  1. Almario, J., Jeena, G., Wunder, J., Langen, G., Zuccaro, A., Coupland, G., and Buche, M., Root-associated fungal microbiota of nonmycorrhizal Arabis alpine and its contribution to plant phosphorus nutrition, Proc. Natl. Acad. Sci. U. S. A., 2017, vol. 114, pp. E9403–E9412. https://doi.org/10.1073/pnas.1710455114

    Article  CAS  Google Scholar 

  2. Capote, N., Del Rio, M.A., Herencia, J.F., and Arroyo, F.T., Molecular and pathogenic characterization of Cylindrocarpon-like anamorphs causing root and basal rot of almonds, Plants, 2022, vol. 11, p. 984. https://doi.org/10.3390/plants11070984

    Article  CAS  Google Scholar 

  3. Chen, C., Verkley, G.J.M., Sun, G., Groenewald, J.Z., and Crous, P.W., Redefining common endophytes and plant pathogens in Neofabraea, Pezicula and related genera, Fungal Biol., 2016, vol. 120, pp. 1291‒1322. https://doi.org/10.1016/j.funbio.2015.09.013

    Article  Google Scholar 

  4. Gardes, M. and Bruns, T.D., ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts, Mol. Ecol., 1993, vol. 2, pp. 113‒118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

    Article  CAS  Google Scholar 

  5. Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/ 98/NT, Nucl. Acid Symp. Ser., 1999, vol. 41, pp. 95‒98.

    CAS  Google Scholar 

  6. Ivanushkina, N.E., Kochkina, G.A., Stupar, O.S., Pavlova, O.S., and Polyanskaya, L.M., Specific features of micromycete complexes of the rhizosphere and nodules of actinorhizal plants, Microbiology (Moscow), 1994, vol. 63, pp. 511‒515.

    Google Scholar 

  7. Khare, E., Mishra, J., and Arora, N.K., Multifaceted interactions between endophytes and plant: developments and prospects, Front. Microbiol., 2018, vol. 9, p. 2732. https://doi.org/10.3389/fmicb.2018.02732

    Article  Google Scholar 

  8. Kochkina, G.A. and Ivanushkina, N.E., Effect of Frankia sp. pigment on growth of fungi from actinorhizal nodules, Wageningen: XII Congress of European Mycologists, 1995, abstracts, p. 31.

  9. Kochkina, G.A., Ivanushkina, N.E., Pavlova, O.S., and Polyanskaya, L.M., Species composition of micromycetes of Alder actinorhizal nodules, Microbiology (Moscow), 1994, vol. 63, pp. 397‒400.

    Google Scholar 

  10. Kornerup, A. and Wanscher J.H., Methuen Handbook of Colour, London: Eyre Methuen, 1978, 3rd ed.

    Google Scholar 

  11. Kowalski, T. and Bartnik, C., Cryptosporiopsis radicicola sp. nov. from roots of Quercus robur, Mycol. Res., 1995, vol. 99, pp. 663‒666. https://doi.org/10.1016/S0953-7562(09)80524-8

    Article  Google Scholar 

  12. Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K., MEGA X: molecular evolutionary genetics analysis across computing platforms, Mol. Biol. Evol., 2018, vol. 35, pp. 1547‒1549. https://doi.org/10.1093/molbev/msy096

    Article  CAS  Google Scholar 

  13. Nei, M. and Kumar, S., Molecular Evolution and Phylogenetics, New York: Oxford Univ. Press, 2000.

    Google Scholar 

  14. Ngom, M., Oshone, R., Diagne, N., Cissoko, M., Svistoonoff, S., Tisa, L.S., Laplaze, L., Sy, M.O., and Champion, A., Tolerance to environmental stress by the nitrogen-fixing actinobacterium Frankia and its role in actinorhizal plants adaptation, Symbiosis, 2016, vol. 70, nos. 1‒3, pp. 17–29. https://doi.org/10.1007/s13199-016-0396-9

    Article  CAS  Google Scholar 

  15. Sarma, K.H., Sarma, B.K., and Tiwari, S.C., A novel calcimycin antibiotic from Gram-positive actinomycete Frankia microsymbiont, Curr. Sci., 2003, vol. 85, pp. 1401‒1403. www.researchgate.net/publication/255621183

    CAS  Google Scholar 

  16. Schulz, B., Sucker, J., Aust, H.J., Krohn, K., Ludewig, K., Jones, P.G., and Döring, D., Biologically active secondary metabolites of endophytic Pezicula species, Mycol. Res., 1995, vol. 99, pp. 1007–1015. https://doi.org/10.1016/S0953-7562(09)80766-1

    Article  CAS  Google Scholar 

  17. Sigler, L., Allan, T., Lim, S.R., Berch, S., and Berbee, M., Two new Cryptosporiopsis species from roots of ericaceous hosts in western North America, Stud. Mycol., 2005, vol. 53, pp. 53‒62. https://doi.org/10.3114/sim.53.1.53

    Article  Google Scholar 

  18. Strobel, G., The emergence of endophytic microbes and their biological promise, J. Fungi, 2018, vol. 4, p. 57. https://doi.org/10.3390/jof4020057

    Article  CAS  Google Scholar 

  19. Stroheker, S., Dubach, V., Vogtli, I., and Sieber, T.N., Investigating host preference of root endophytes of three European tree species, with a focus on members of the Phialocephala fortinii‒Acephala applanata species complex (PAC), J. Fungi, 2021, vol. 7, p. 317. https://doi.org/10.3390/jof7040317

    Article  Google Scholar 

  20. Tedersoo, L., Jairus, T., Horton, B.M., Abarenkov, K., Suvi, T., Saa, I., and Kõljalg, U., Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon-specific primers, New Phytol., 2008, vol. 180, pp. 479‒490. https://doi.org/10.1111/j.1469-8137.2008.02561.x

    Article  CAS  Google Scholar 

  21. Toju, H. and Sato, H., Root-associated fungi shared between arbuscular mycorrhizal and ectomycorrhizal conifers in a temperate forest, Front. Microbiol., 2018, vol. 9, p. 433. https://doi.org/10.3389/fmicb.2018.00433

    Article  Google Scholar 

  22. Toju, H., Tanabe, A.S., and Sato, H., Network hubs in root-associated fungal metacommunities, Microbiome, 2018, vol. 6, p. 116. https://doi.org/10.1186/s40168-018-0497-1

    Article  Google Scholar 

  23. Tsiknia, M., Tsikou, D., Papadopoulou, K., and Ehaliotis, C., Multi-species relationships in legume roots: from pairwise legume-symbiont interactions to the plant–microbiome–soil continuum, FEMS Microbiol. Ecol., 2021, vol. 97, fiaa222. https://doi.org/10.1093/femsec/fiaa222

  24. Vasilenko, O., Ivanushkina, N., Starodumova, I., Kochkina, G., Danilogorskaya, A., Pinchuk, I., Telkov, M., and Ozerskaya, S., The advantage of the usage of the long “boosted” fungal barcode that includes ITS1/2 region and D1/D3 domain of the  26s  large  subunit  ribosomal  DNA, Proc. 37th Ann. Meet. Euro. Cult. Collect. Org., Moscow, 2018, pp. 126‒127.

  25. Verkley, G.J.M., Zijlstra, J.D., Summerbell, R.C., and Berendse, F., Phylogeny and taxonomy of root-inhabiting Cryptosporiopsis species, and C. rhizophila sp. nov., a fungus inhabiting roots of several Ericaceae, Mycol. Res., 2003, vol. 107, pp. 689‒698. https://doi.org/10.1017/S0953756203007883

    Article  CAS  Google Scholar 

  26. Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V., and Verkley, G.J.M., Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation, Stud. Mycol., 2019, vol. 92, pp. 135‒154. https://doi.org/10.1016/j.simyco.2018.05.001

    Article  CAS  Google Scholar 

  27. Wang, J., Wang, G., Zhang, Y., Zhen, B., Zhang, C., and Wang, L., Isolation and identification of an endophytic fungus Pezicula sp. in Forsythia viridissima and its secondary metabolites, World J. Microb. Biot., 2014, vol. 30, pp. 2639–2644. https://doi.org/10.1007/s11274-014-1686-0

    Book  Google Scholar 

  28. Wang, W., Tsuneda, A., Gibas, C., and Currah, R.S., Cryptosporiopsis species isolated from the roots of aspen in central Alberta: identification, morphology and interactions with the host, in vitro, Can. J. Bot., 2007, vol. 85, pp. 1214–1226. https://doi.org/10.1139/B07-086

    Article  CAS  Google Scholar 

  29. Xu, L., Duan, X., Wei, X., Xue, J., Feng, L., and Wu, P., Fungal trisaccharide ester compounds and use thereof in preparing agents for preventing and controlling plant fungal diseases, US Patent, no. 0166835 A1, 2019.

  30. Yue, Q., Li, Y., Chen, L., Zhang, X., Liu, X., An, Z., and Bill, G.F., Genomics driven discovery of a novel self-resistance mechanism in the echinocandin producing fungus Pezicula radicicola, Environ. Microbiol., 2018, vol. 20, pp. 3154–3167. https://doi.org/10.1111/1462-2920.14089

    Article  CAS  Google Scholar 

  31. Zhang, X.H., Zhang, D.J., Liu, J.L., Pan, H.Y., Qin, J.C., and Zhang, Y.H., Antifungal effects of volatile organic compounds from the endophytic fungus Cryptosporiopsis ericae Cc-HG-7 isolated from Coptis chinensis Franch., Biocontrol. Sci. Techn., 2018, vol. 28, pp. 496‒508. https://doi.org/10.1080/09583157.2018.1460744

    Article  Google Scholar 

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Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation, project no. 075-15-2021-1051.

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Authors and Affiliations

  1. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences (PSCBR RAS), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia

    G. A. Kochkina, N. E. Ivanushkina, I. P. Pinchuk & S. M. Ozerskaya

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  1. G. A. Kochkina
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  2. N. E. Ivanushkina
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  3. I. P. Pinchuk
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  4. S. M. Ozerskaya
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Correspondence to G. A. Kochkina.

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Translated by E. Babchenko

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Kochkina, G.A., Ivanushkina, N.E., Pinchuk, I.P. et al. Endophytic Fungi Pezicula radicicola in the Root Nodules of Actinorhizal Plants. Microbiology 91, 750–756 (2022). https://doi.org/10.1134/S0026261722601622

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  • DOI: https://doi.org/10.1134/S0026261722601622

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