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The Effects of Lactic Acid Bacteria on Salmonella Biofilms

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Abstract

The anti-biofilm effects of the following probiotic LAB strains: Lactobacillus plantarum M16 (producer of plantarisin), Lactobacillus sake M17 (producer of sakacin), Pediococcus acidilactici M20 (producer of pediocin), Bifidobacterium longum M23 (bifidosin A producer strain), Bifidobacterium bifidum M24 (bifidocin B producer strain), and Pediococcus pentosaceus M46 (bacteriocin like substance producer strain) on the formation and eradication of mature Salmonella Typhimurium 14 028 biofilms were investigated. The strains were previously isolated from fermented foods traditionally produced in Turkey and identified. Biofilm formation by Salmonella Typhimurium 14 028 was completely inhibited after treatment with the cell-free filtrates of lactic acid bacteria. The amount of mature biofilms dropped at statistically significant levels after six hours (p < 0.05) only as the result of treatment with Pediococcus pentosaceus M46 cell-free filtrate, rather than with other cell-free filtrates. On the other hand, the cell-free filtrates of the other LAB strains promoted biofilm maturation. Biofilm assays were carried out with neutralized cell-free filtrates and neutralized cell-free filtrates treated with proteinase K to determine the source of the antibiofilm activity. The antibiofilm activities that were initially determined dropped statistically significantly (p < 0.05) as a result of both treatments. Based on these findings, the probiotic strains isolated from Turkey exhibited particularly significant antibiofilm capabilities, which were mostly due to the bacteriocins produced by these strains.

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ACKNOWLEDGMENTS

We would like to thank Prof. Hilmi Volkan Demir (National Nanotechnology Center, Bilkent University, UNAM), who let us work with a scanning electron microscope.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ankara University, Emniyet Mah., Ankara, Turkey

    Ş. Göksel, C. Özdemir & M. Akçelik

  2. Institute of Biotechnology, Ankara University, Gümüşdere, Ankara, Turkey

    N. Akçelik

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  1. Ş. Göksel
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Statement on the welfare of animals. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHORS CONTRIBUTION

M. Akçelik participated in designing and performing experiments, processing, and interpreting data, and preparing, writing, and revising the manuscript. Ş. Göksel participated in performing experiments, processing data, and preparation of the manuscript. N. Akçelik assisted in designing experiments, processing, and interpreting the data. C. Özdemir participated in biofilm assays, scanning electron microscopic examinations and processing data.

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Göksel, Ş., Akçelik, N., Özdemir, C. et al. The Effects of Lactic Acid Bacteria on Salmonella Biofilms. Microbiology 91, 278–285 (2022). https://doi.org/10.1134/S0026261722300129

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