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Characterization of the Novel Plant Growth-Stimulating Strain Advenella kashmirensis IB-K1 and Evaluation of Its Efficiency in Saline Soil

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Abstract—

The strain of facultative chemolithotrophic bacteria isolated from soil within the aphotic zone of the Kinderlinskaya Cave (Southern Urals, Russia) was identified as member of the species Advenella kashmirensis based on its phenotypic characteristics and sequencing of its 16S rRNA gene fragment. The novel isolate was similar to the type strain A. kashmirensis WT 001 in its ability to utilize thiosulfate as an energy source and to grow in the presence of 7% NaCl. The strain A. kashmirensis IB-K1 actively solubilized poorly soluble organic and mineral phosphorous compounds in in vitro experiments (solubilization indexes for phytin and calcium orthophosphate were 7 and 5, respectively). Moreover, these bacterial cells exhibited high activity of alkaline and acid phosphatases. Scanning electron microscopy of 4-day-old wheat seedling roots revealed active colonization of their rhizoplane by A. kashmirensis IB-K1 in the root hairs zone. The main mechanism of plant growth-promoting effect caused by the strain IB-K1 is probably associated with its phosphate-mobilizing activity and indoleacetic acid (IAA) production (up to 220 ± 42 ng/mL). Presowing treatment of wheat (Triticum durum Desf.) seeds with A. kashmirensis strain IB-K1 significantly alleviated the adverse effect of salinity stress on plant growth under moderate salinization level of cultured soil (at content of water-soluble salts 0.33 ± 0.06%), resulting in improvement of some parameters of yield structure and increased plant productivity. These findings are the first demonstration of the possible application of Advenella species in agricultural crop protection and plant growth stimulation under salinity stress.

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ACKNOWLEDGMENTS

The authors are grateful to I.I. Musabirov (Institute for Metals Superplasticity Problems, Russian Academy of Sciences, Ufa) for his assistance in conducting SEM studies.

Funding

This work was supported by the state budget of the Ministry of Education and Science of the Russian Federation, project no. AAAA-A18-118022190098-9 “Ecological and Genetic-Physiological Features of the Interaction of Species in Natural and Artificial Communities of Microorganisms,” as well as by the Russian Foundation for Basic Research, project no. 18-04-00577/18 “The Ability of Halotolerant Bacteria to Produce Phytohormones and Influence the Salt Tolerance of Plants.” The study was conducted using the equipment of the Agidel Center for Collective Use of the Ufa Federal Research Center, Russian Academy of Sciences.

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  1. Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences, 450054, Ufa, Russia

    L. Yu. Kuzmina, E. A. Gilvanova, N. F. Galimzyanova, T. N. Arkhipova, A. S. Ryabova, G. E. Aktuganov, L. V. Sidorova, G. R. Kudoyarova & A. I. Melent’ev

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  1. L. Yu. Kuzmina
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  2. E. A. Gilvanova
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  3. N. F. Galimzyanova
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Correspondence to L. Yu. Kuzmina.

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Kuzmina, L.Y., Gilvanova, E.A., Galimzyanova, N.F. et al. Characterization of the Novel Plant Growth-Stimulating Strain Advenella kashmirensis IB-K1 and Evaluation of Its Efficiency in Saline Soil. Microbiology 91, 173–183 (2022). https://doi.org/10.1134/S0026261722020072

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