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Cell Wall Rhamnan in Actinobacteria of the Genus Curtobacterium

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Abstract—

Presence of the cell wall glycopolymer rhamnan was established for members of the genus Curtobacterium (family Microbacteriaceae) by using chemical and NMR spectroscopic methods. The rhamnan with the repeating unit, →2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→, was found in C. flaccumfaciens pv. flaccumfaciens VKM Ас-1923T, C. flaccumfaciens pv. poinsettiae VKM Ас-1924T, C. pusillum VKM Ac-2099T, C. herbarum VKM Ac-2512T, and Curtobacterium sp. VKM Ac-1811. Each of the studied strains also contained a second, acidic phosphate-free polysaccharide. NMR spectra of these polysaccharides differed, indicating the structural differences. Cell wall hydrolysates of all studied strains were found to contain rhamnose, mannose, glucose, galactose, and glucosamine. Further research will facilitate more detailed characterization of the composition of cell wall glycopolymers in Curtobacterium species, as well as evaluation of their taxonomic significance. The data on the polymer structure will contribute to understanding of the mechanisms of colonization and infection of higher organisms by Curtobacterium species and bacterial interaction in microbial communities and with abiotic environmental objects.

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Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation.

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    V. A. Zaychikov, N. V. Potekhina, Ding Fan & E. M. Tul’skaya

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. S. Dmitrenok

  3. All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    L. V. Dorofeeva & L. I. Evtushenko

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  1. V. A. Zaychikov
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  2. N. V. Potekhina
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Correspondence to N. V. Potekhina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Makeeva

Abbreviations: HSQC, proton-detected heteronuclear single quantum correlation; J, spin-spin interaction constant; ROESY, Rotating-frame nuclear Overhauser Effect correlation Spectroscopy; COSY, Correlated Spectroscopy; TOCSY, Total Correlation Spectroscopy; HMBC, Heteronuclear Multiple Bond Correlation; δС, δН, the values of chemical shifts of the 13C and 1H atoms, respectively.

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Zaychikov, V.A., Potekhina, N.V., Dmitrenok, A.S. et al. Cell Wall Rhamnan in Actinobacteria of the Genus Curtobacterium . Microbiology 90, 343–348 (2021). https://doi.org/10.1134/S0026261721030139

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