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Carbonic Anhydrase in Anoxygenic Phototrophic Bacteria

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Abstract—

The genes encoding carbonic anhydrase (CA) were found in all anoxygenic purple bacteria. The genes of α- and β-CA were found in purple nonsulfur bacteria of the class Alphaproteobacteria:Rhodospirillum rubrum, Rhodospirillum fulvum, Rhodoblastus acidophilus, and Rhodopseudomonas palustris. The alphaproteobacteria Rhodomicrobium vannielii, Blastochlorisviridis, Rhodobacter sphaeroides, Rhodobacter capsulatus, Rhodobacter veldkampii, Rhodovulumeuryhalinum, and Rhodovulum sulfidophilum possessed only the β-CA genes. Both nonsulfur purple bacteria of the class Betaproteobacteria (Rubrivivax gelatinosus) and purple sulfur bacteria (class Gammaproteobacteria) contained the α- and β-CA. No CA genes were found in green sulfur bacteria Chlorobaculum limnaeum and Chlorobaculum parvum, as well as in filamentous green nonsulfur bacteria Chloroflexus aurantiacus. However, the β-CA gene was revealed in Oscillochloris trichoides, which belonged to the latter taxonomic group. No γ-CA genes were detected in the genomes of the phototrophic bacteria studied in the present work. Although CA genes were present in all purple bacteria, the α- and β-CA activity was observed only in four species of purple nonsulfur Alphaproteobacteria: Rhodospirillum rubrum, Rhodopseudomonas palustris, Rhodoblastusacidophilus, and Rhodospirillum fulvum. These bacteria are able to synthesize CA under both photoautotrophic and photoheterotrophic conditions in the media with acetate, malate, or fructose. These bacteria (except for Rhodospirillum fulvum, which is unable to grow under aerobic conditions), also exhibit CA activity when grown under aerobic conditions in the dark.

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Authors and Affiliations

  1. Department of Microbiology, Biological Faculty, Moscow State University, 119192, Moscow, Russia

    R. N. Ivanovsky, O. I. Keppen & N. V. Lebedeva

  2. Laboratory of Molecular Diagnostics, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    D. S. Gruzdev

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  1. R. N. Ivanovsky
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  2. O. I. Keppen
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  4. D. S. Gruzdev
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Correspondence to R. N. Ivanovsky.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Babchenko

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Ivanovsky, R.N., Keppen, O.I., Lebedeva, N.V. et al. Carbonic Anhydrase in Anoxygenic Phototrophic Bacteria. Microbiology 89, 266–272 (2020). https://doi.org/10.1134/S0026261720020058

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