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Thermophilic Bacteria in Lake Baikal Bottom Sediments Associated with Hydrocarbon Discharge

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Abstract—

The research was aimed at detection of thermophilic microorganisms in Lake Baikal low-temperature sediments associated with discharge of gas-saturated fluids. Members of the order Clostridiales were revealed in enrichment cultures obtained from the bottom sediments at three sites (methane seep, oil-methane seep, and mud volcano). No thermophilic prokaryotes were found in the enrichment culture with sediment samples from a background area. The presence of thermophilic microorganisms at the sites of hydrocarbon discharge may result from their migration to the bottom surface with the gas-bearing mineralized fluid moving along the fracture zones. Unlike marine cold sediments, where the endospores of thermophilic bacteria belong to strict anaerobes existing due to fermentation of organic substrates or sulfate reduction, Lake Baikal sediments associated with discharge of gas-saturated fluids were found to contain facultatively anaerobic thermophilic prokaryotes.

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REFERENCES

  1. Belkova, N.L., Parfenova, V.V., Suslova, M.Yu., Ahn, T.S., and Tazaki, K., Biodiversity and activity of the microbial community in the Kotelnikovsky hot springs (Lake Baikal), Biol. Bull. (Moscow), 2005, vol. 32, pp. 549–555.

    Article  Google Scholar 

  2. Bonch-Osmolovskaya, E.A., Metabolic diversity of thermophilic prokaryotes–what’s new?, in Extremophiles: Microbiology and Biotechnology, Anitori, R., Ed., Beaverton: Horizon, 2012, pp. 109–130.

    Google Scholar 

  3. Chakraborty, A., Ellefson, E., Li, C., Gittins, D., Brooks, J.M., Bernard, B.B., and Hubert, C.R.J., Thermophilic endospores associated with migrated thermogenic hydrocarbons in deep Gulf of Mexico marine sediments, ISME J., 2018, vol. 12, pp. 1895–1906.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Chernitsyna, S.M., Mamaeva, E.V., Lomakina, A.V., Pogodaeva, T.V., Galach’yants, Yu.P., Bukin, S.V., Pimenov, N.V., Khlystov, O.M., and Zemskaya, T.I., Phylogenetic diversity of microbial communities of the Posolsk Bank bottom sediments, Lake Baikal, Microbiology (Moscow), 2016, vol. 85, pp. 672–680.

    Article  CAS  Google Scholar 

  5. Cuylaerts, M., Naudts, L., Casier, R., Khabuev, A.V., Belousov, O.V., Kononov, E.E., Khlystov, O., and De Batist, M., Distribution and morphology of mud volcanoes and other fluid flow-related lake-bed structures in Lake Baikal, Russia, Geo-Mar. Lett., 2012, vol. 32, pp. 383–394.

    Article  Google Scholar 

  6. De Rezende, J.R., Kjeldsen, K.U., Hubert, C.R.J., Finster, K., Loy, A., and Jørgensen, B.B., Dispersal of thermophilic Desulfotomaculum endospores into Baltic Sea sediments over thousands of years, ISME J., 2013, vol. 7, pp. 72–84.

    Article  CAS  PubMed  Google Scholar 

  7. Duchkov, A.D., Lysak, S.V., Golubev, V.A., Dorofeeva, R.P., and Sokolova, L.S., Heat flow and geotemperature fiald of the Baikal region, Geol. Geofiz., 1999, vol. 40, no. 3, pp. 287–303.

    Google Scholar 

  8. Fardeau, M.-L., Barsotti, V., Cayol, J.-L., Guasco, S., Michotey, V., Joseph, M., Bonin, P., and Ollivier, B., Caldinitratiruptor microaerophilus, gen. nov., sp. nov. isolated from a French hot spring (Chaudes-Aigues, Massif Central): a novel cultivated facultative microaerophilic anaerobic thermophile pertaining to the Symbiobacterium branch within the Firmicutes, Extremophiles, 2010, vol. 14, pp. 241–247.

    Article  CAS  PubMed  Google Scholar 

  9. Golubev, V.A., Heat flow through Lake Baikal depression, Doklady AN SSSR, 1979, vol. 245, no. 6, pp. 1333–1336.

    Google Scholar 

  10. Han, C., Gu, W., Zhang, X., Lapidus, A., Nolan, M., Copeland, A., Lucas, S., Del Rio, T.G., Tice, H., Cheng, J.F., Tapia, R., Goodwin, L., Pitluck, S., Pagani, I., Ivanova, N., et al., Complete genome sequence of Thermaerobacter marianensis type strain (7p75a), Stand. Genom. Sci., 2010, vol. 3, pp. 337–345.

    Google Scholar 

  11. Hubert, C., Arnosti, C., Brüchert, V., Loy, A., Vandieken, V., and Jørgensen, B.B., Thermophilic anaerobes in Arctic marine sediments induced to mineralize complex organic matter at high temperature, Environ. Microbiol., 2010, vol. 12, pp. 1089–1104.

    Article  CAS  PubMed  Google Scholar 

  12. Hubert, C., Loy, A., Nickel, M., Arnosti, C., Baranyi, C., Brüchert, V., Ferdelman, T., Finster, K., Christensen, F.M., De Rezende, J.R., Vandieken, V., and Jørgensen, B.B., A constant flux of diverse thermophilic bacteria into the cold Arctic seabed, Science, 2009, vol. 325, pp. 1541–1544.

    Article  CAS  PubMed  Google Scholar 

  13. Isaksen, M.F., Bak, F., and Jørgensen, B.B., Thermophilic sulfate-reducing bacteria in cold marine sediment, FEMS Microbiol. Ecol., 1994, vol. 14, pp. 1–8.

    Article  CAS  Google Scholar 

  14. Kalashnikov, A.M., Gaisin, V.A., Sukhacheva, M.V., Namsaraev, B.B., Panteleeva, A.N., Nuyanzina-Boldareva, E.N., Kuznetsov, B.B., and Gorlenko, V.M., Anoxygenic phototrophic bacteria from microbial communities of Goryachinsk, Microbiology (Moscow), 2014, vol. 83, pp. 407–421.

    Article  CAS  Google Scholar 

  15. Khlystov, O.M., De Batist, M., Shoji, H., Hachikubo, A., Nishio, S., and Naudt, L., Gas hydrate of Lake Baikal: discovery and varieties, J. Asian. Earth Sci., 2013, vol. 62, pp. 162–166.

    Article  Google Scholar 

  16. Khlystov, O.M., Minami, H., Hachikubo, A., Yamashita, S., De Batist, M., Nauds, L., Khabuev, A.V., Chenskiy, A.G., Gubin, N.A., and Vorobyeva, S.S., Age of mud breccia from mud volcanoes in Academician Ridge, Lake Baikal, Geodyn. Tectonophys., 2017, vol. 8, pp. 923–932.

    Article  Google Scholar 

  17. Klerkx, J., De Batist, M., Poort, J., Hus, R., VanRensbergen, P., Khlystov, O.M., and Granin, N., Tectonically controlled methane escape in Lake Baikal, in Advances in the Geological Storage of Carbon Dioxide, NATO Sci. Ser. IV. Earth Environ. Sci., 2006, vol. 65, pp. 203–219.

    Google Scholar 

  18. Kontorovich, A.E., Kashirtsev, V.A., Moskvin, V.I., Burshtein, L.M., Zemskaya, T.I., Kostyreva, E.A., Kalmychkov, G.V., and Khlystov, O.M., Petroleum potential of Baikal deposits, Russ. Geol. Geophys. (Novosibirsk), 2007, vol. 12, pp. 1046–1053.

    Article  Google Scholar 

  19. Kovaleva, O.L., Merkel, A.Y., Novikov, A.A., Baslerov, R.V., Toshchakov, S.V., and Bonch-Osmolovskaya, E.A., Tepidisphaera mucosa gen. nov., sp. nov., a moderately thermophilic member of the class Phycisphaerae in the phylum Planctomycetes, and proposal of a new family, Tepidisphaeraceae fam. nov., and a new order, Tepidisphaerales ord. nov., Int. J. Syst. Evol. Microbiol., 2015, vol. 65, pp. 549–555.

    Article  CAS  PubMed  Google Scholar 

  20. Kozhov, M., Geography and hydrology of Baikal, in Lake Baikal and Its Life. Monographiae Biologicae, Dordrecht: Springer, 1963, vol. 11, pp. 5–55.

    Book  Google Scholar 

  21. Kuzmin, M.I., Karabanov, E.B., Kawai, T., Williams, D., Bychinsky, V.A., Kerber, E.V., Kravchinsky, V., Bezrukova, E., Prokopenko, A.A., Geletii, V.F., Kalmychkov, G.V., Goreglyad, A.V., Antipin, V.S., Khomutova, M.Yu., Soshina, N.M., et al., Deep drilling on Lake Baikal: main results, Russ. Geol. Geophys. (Novosibirsk), 2001, vol. 42, pp. 8–34.

    CAS  Google Scholar 

  22. Lane, D.J., 16S/23S rRNA sequencing, in Nucleic Acid Techniques in Bacterial Systematics, Stackebrandt, E. and Goodfellow, M., Eds., New York: Wiley, 1991, pp. 115–175.

  23. Lavrenteva, E.V., Shagzhina, A.P., Babasanova, O.B., Dunaevsky, Y.E., Barkhutova, D.D., and Namsaraev, Z.B., The study of two alkaliphilic thermophile bacteria of the Anoxybacillus genus, Appl. Biochem. Microbiolol., 2009, vol. 45, pp. 484–488.

    Article  CAS  Google Scholar 

  24. Lebedeva, E.V., Of, S., Zumbragel, S., Kruse, M., Shagzhina, A., Lücker, S., Maixner, F., Lipski, A., Daims, H., and Spieck, E., Isolation and characterization of a moderately thermophilic nitrite-oxidizing bacterium from a geothermal spring, FEMS Microbiol. Ecol., 2010, vol. 75, pp. 195–204.

    Article  CAS  PubMed  Google Scholar 

  25. Müller, A.L., De Rezende, J.R., Hubert, C.R.J., Kjeldsen, K.U., Lagkouvardos, I., Berry, D., Jørgensen, B.B., and Loy, A., Endospores of thermophilic bacteria as tracers of microbial dispersal by ocean currents, ISME J., 2014, vol. 8, pp. 1153–1165.

    Article  CAS  PubMed  Google Scholar 

  26. Naudts, L., Khlystov, O., Granin, N., Chensky, A., Poort, J., and De Batist, M., Stratigraphic and structural control on the distribution of gas hydrates and active gas seeps on the Posolsky Bank, Lake Baikal, Geo-Mar. Lett., 2012, vol. 32, pp. 395–406.

    Article  CAS  Google Scholar 

  27. Pavlova, O.N., Bukin, S.V., Gorshkov, A.G., Khanaeva, T.A., and Zemskaya, T.I., Microorganisms of Lake Baikal: from psychrophilic hydrocarbon-oxidizing aerobed to thermophilic mixotrophs, 1 Rossiiskii mikrobiologicheskii kongress (1st Russian Microbiological Congress), Reshetilova, T.A., Ed., Moscow: Voda: Khim. Ekol., 2017, pp. 68–69.

  28. Pogodaeva, T.V., Lopatina, I.N., Khlystov, O.M., Egorov, A.V., and Zemskaya, T.I., Background composition of pore waters in Lake Baikal bottom sediments, J. Great Lakes Res., 2017, vol. 43, pp. 1030–1043.

    Article  CAS  Google Scholar 

  29. Pogodaeva, T.V., Zemskaya, T.I., Golobokova, L.P., Khlystov, O.M., Minami, H., and Sakagami, H., Chemical composition of pore waters of bottom sediments in different Baikal basins, Russ. Geol. Geophys. (Novosibirsk), 2007, vol. 48, pp. 886–900.

    Article  Google Scholar 

  30. Poort, J., Khlystov, O.M., Naudts, L., Duchkov, A.D., Shoji, H., Nishio, S., De Batist, M., Hachikubo, A., Kida, M., Minami, H., Manakov, A.Y., Kulikova, M.V., and Krylov, A.A., Thermal anomalies associated with shallow gas hydrates in the K-2 mud volcano, Lake Baikal, Geo-Mar. Lett., 2012, vol. 32, pp. 407–417.

    Article  CAS  Google Scholar 

  31. Praktikum po mikrobiologii (Practical Course in Microbiology), Netrusov, A.I., Ed., Moscow: Akademiya, 2005.

    Google Scholar 

  32. Radnagueva, A.A., Lavrentieva, E.V., Budagaeva, V.G., Barkhutova, D.D., and Namsaraev, B.B., Organotrophic bacteria of the Baikal Rift Zone hot springs, Microbiology (Moscow), 2016, vol. 85, pp. 367–378.

    Article  CAS  Google Scholar 

  33. Rozanov, A.S., Bryanskaya, A.V., Kotenko, A.V., and Peltek, S.E., Draft genome sequence of Thermoactinomyces sp. Gus2-1 isolated from the hot-spring Gusikha in Bargusin Valley (Baikal Rift Zone, Russia), Genomics Data, 2017, vol. 11, pp. 1–2. https://doi.org/10.1016/j.gdata.2016.11.014

    Article  PubMed  Google Scholar 

  34. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning. A Laboratory Manual, New York: Cold Spring Harbor, 1989, vol. 2.

  35. Sokolova, T.G., Kostrikina, N.A., Chernyh, N.A., Kolganova, T.V., Tourova, T.P., and Bonch-Osmolovskaya, E.A., Thermincola carboxydiphila gen. nov., sp. nov., a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area, Int. J. Syst. Evol. Microbiol., 2005, vol. 55, pp. 2069–2073.

    Article  CAS  PubMed  Google Scholar 

  36. Spanevello, M.D. and Patel, B.K.C., Thermaerobacter, in Bergey’s Manual of Systematics of Archaea and Bacteria, Whitman, W.B., Ed., Wiley, 2015. https://doi.org/10.1002/9781118960608.gbm00730

  37. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 2011, vol. 28, pp. 2731–2739.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Van Rensbergen, P., De Batist, M., Klerkx, J., Hus, R., Poort, J., Vanneste, M., Granin, N., Khlystov, O., and Krinitsky, P., Sublacustrine mud volcanoes and methane seeps caused by dissociation of gas hydrates in Lake Baikal, Geology, 2002, vol. 30, pp. 631–634.

    Article  CAS  Google Scholar 

  39. Verbolov, V.I., Currents and water exchange in Lake Baikal, Water Res., 1996, vol. 23, no. 4, pp. 381–391.

    CAS  Google Scholar 

  40. Volpi, M., Lomstein, B.A, Sichert, A., Roy, H., Jorgensen, B.B., and Kjeldsen, K.U., Identity, abundance, and reactivation kinetics of thermophilic fermentative endospores in cold marine sediment and seawater, Front. Microbiol., 2017, vol. 8, p. 131. https://doi.org/10.3389/fmicb.2017.00131

    Article  PubMed  PubMed Central  Google Scholar 

  41. Yu, Z., Wu, C., Yang, G.Q., and Zhou, S.G., Planifilum caeni sp. nov., a novel member of Thermoactinomycete isolated from sludge compost, Curr. Microbiol., 2015, vol. 70, pp. 135–140.

    Article  CAS  PubMed  Google Scholar 

  42. Zhilina, T.N., Kevbrin, V.V., Tourova, T.P., Lysenko, A.M., Kostrikina, N.A., and Zavarzin, G.A., Clostridium alkalicellum sp. nov., an obligately alkaliphilic cellulolytic bacterium from a soda lake in the Baikal Region, Microbiology (Moscow), 2005, vol. 74, pp. 557–566.

    Article  CAS  Google Scholar 

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FUNDING

This work was carried out within the framework of the State Assignment no. 0345-2019-0007 (expedition studies) and 0345-2018-0001 (lithologic investigations and chemical analysis of pore waters) and was supported by the Russian Foundation for Basic Research, project no. 16-04-00181_а (microbiological and molecular biological studies).

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Authors and Affiliations

  1. Limnological Institute, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia

    O. N. Pavlova, A. V. Lomakina, A. S. Novikova, S. M. Chernitsyna, T. A. Khanaeva, T. V. Pogodaeva, A. V. Khabuev & T. I. Zemskaya

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  1. O. N. Pavlova
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  2. A. V. Lomakina
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  3. A. S. Novikova
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  4. S. M. Chernitsyna
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  5. T. A. Khanaeva
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  6. T. V. Pogodaeva
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  7. A. V. Khabuev
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  8. T. I. Zemskaya
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Correspondence to O. N. Pavlova.

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Translated by E. Dedyukhina

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Pavlova, O.N., Lomakina, A.V., Novikova, A.S. et al. Thermophilic Bacteria in Lake Baikal Bottom Sediments Associated with Hydrocarbon Discharge. Microbiology 88, 335–342 (2019). https://doi.org/10.1134/S0026261719030081

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