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Rhizobacteria Strain from a Hypersaline Environment Promotes Plant Growth of Kengyilia thoroldiana

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Abstract

Kengyilia thoroldiana is a nutritionally rich grass species of the Qinghai-Tibet Plateau. Here, to improve its quality and biomass via biological fertilization, we sought out plant-growth promoting rhizobacteria. Our screening found one Bacillus species capable of nitrogen fixation, indole-3-acetic acid production, and tolerating the extreme saline-alkali soil of Qinghai Province. We determined the strain’s growth performance and antagonistic effects with pathogens (Fusarium graminearum, F. acuminatum) and evaluated its impact on K. thoroldiana. The test strain KKLW1 was identified as Bacillusamyloliquefaciens by morphology, adversity culture, and 16S rDNA and gyrB partial sequence analyses. KKLW1 strongly tolerates saline conditions (11% salt, pH 11), and showed stable nitrogen fixation activity and IAA production capacity; its Phl gene, which we amplified, was significant antagonistic to pathogenic pasture fungi. Furthermore, compared with the control, the strain showed clear germination and growth-promoting activity after soaking grass seeds and root-irrigating with the Bacillus suspension, increasing each by 8 and 10–12%, respectively. In sum, the strain KKLW1 has excellent biological properties and stable physiological characteristics in this extreme environment of the Qinghai-Tibet Plateau. We propose KKLW1 for use as a functional strain of microbial fertilizer to increase production and restore vegetation coverage of the Plateau’s grassland.

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REFERENCES

  1. Ali, R.M., Houghton, P.J., and Hoo, T.S., Antifungal activity of some bignoniaceae found in malaysia, Phytother. Res., 2015, vol. 12, pp. 331‒334.

    Article  Google Scholar 

  2. Bhadury, P. and Austen, M.C., Barcoding marine nematodes: an improved set of nematode 18S rRNA primers to overcome eukaryotic co-interference, Hydrobiologia, 2010, vol. 641, pp. 245‒251.

    Article  CAS  Google Scholar 

  3. Chen, L., Liu, Y., Wu, G., Veronican, Njeri. K., Shen, Q., Zhang, N., and Zhang, R., Induced maize salt tolerance by rhizosphere inoculation of Bacillus amyloliquefaciens SQR9, Physiol. Plant., 2016, vol. 158, pp. 34‒44.

    Article  CAS  PubMed  Google Scholar 

  4. Das, K., and Mukherjee, A.K., Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from north-east India, Bioresour. Technol., 2007, vol. 98, pp. 1339‒1345.

    Article  CAS  PubMed  Google Scholar 

  5. Dong, Q., Zhao, X., Wu, G., Shi, J., and Ren, G., A review of formation mechanism and restoration measures of “black-soil-type” degraded grassland in the Qinghai-Tibetan Plateau, Environ. Earth Sci., 2013, vol. 70, pp. 2359‒2370.

    Article  Google Scholar 

  6. Dong, Q.M., Zhao, X.Q., Wu, G.L., and Chang, X.F., Optimization yak grazing stocking rate in an alpine grassland of Qinghai-Tibetan Plateau, China. Environ. Earth Sci., 2015, vol. 73, pp. 2497‒2503.

    Article  Google Scholar 

  7. Dong, S.K., Wen, L., Li, Y.Y., Wang, X.X., Zhu, L., and Li, X.Y., Soil-quality effects of grassland degradation and restoration on the Qinghai-Tibetan Plateau, Soil Sci. Soc. Am. J., 2012, vol. 76, pp. 2256‒2264.

    Article  CAS  Google Scholar 

  8. Duarte, G.R.M., Price, C.W., Augustine, B.H., Carrilho, E., and Landers, J.P., Dynamic solid phase DNA extraction and PCR amplification in polyester-toner based microchip, Anal. Chem., 2011, vol. 83, pp. 5182‒5189.

    Article  CAS  PubMed  Google Scholar 

  9. Eriksen, J., Pedersen, L., and Jorgensen, J.R., Nitrate leaching and bread-making quality of spring wheat following cultivation of different grasslands, Agric., Ecosyst. Environ., 2006, vol. 116, pp. 165‒175.

    Article  CAS  Google Scholar 

  10. Fan, B., Chen, X.H., Budiharjo, A., Bleiss, W., Vater, J., and Borriss, R., Efficient colonization of plant roots by the plant growth promoting bacterium Bacillus amyloliquefaciens FZB42, engineered to express green fluorescent protein, J. Biotechnol., 2011, vol. 151, pp. 303‒311.

    Article  CAS  PubMed  Google Scholar 

  11. Fang, X.Z., Deng, W., Yuan, Y.W., and Zhao, C.H., The impacts of climate change and human activities on grassland productivity in Qinghai Province, China, Front. Earth Sci., 2014, vol. 8, pp. 93‒103.

    Article  Google Scholar 

  12. Gaiero, J.R., and Dunfield, K.E., Inside the root microbiome: bacterial root endophytes and plant growth promotion, Am. J. Bot., 2013, vol. 100, pp. 1738‒1750.

    Article  PubMed  Google Scholar 

  13. Gong, G., Kim, S., Lee, S.M., Woo, H.M., Park, T.H., and Um, Y., Complete genome sequence of Bacillus sp. 275, producing extracellular cellulolytic, xylanolytic and ligninolytic enzymes, J. Biotechnol., 2017, vol. 254, pp. 59‒62.

    Article  CAS  PubMed  Google Scholar 

  14. Idris,E.E., Iglesias, D.J., Talon, M., and Borriss, R., Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42, Mol. Plant-Microbe Interact., 2007, vol. 20, pp. 619‒626.

    Article  CAS  PubMed  Google Scholar 

  15. Karlidag, H., Esitken, A., Turan, M., and Sahin, F., Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple, Sci. Hortic., 2007, vol. 114, pp. 16‒20.

    Article  CAS  Google Scholar 

  16. Kayalvizhi, N. and Gunasekaran, P., Production and characterization of a low-molecular-weight bacteriocin from Bacillus licheniformis, MKU3, Lett. Appl. Microbiol., 2008, vol. 47, pp. 600‒607.

    Article  CAS  PubMed  Google Scholar 

  17. Kizilkaya, R., Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils, J. Environ. Biol., 2009, vol. 30, pp. 73‒82.

    CAS  PubMed  Google Scholar 

  18. Kloepper, J.W., Ryu, C.M., and Zhang, S., Induced systemic resistance and promotion of plant growth by Bacillus spp., Phytopathology, 2004, vol. 94, pp. 1259‒1266.

    Article  CAS  PubMed  Google Scholar 

  19. Li, C.H., Li, S.J., Lei, Y.S., Hu, K., Wang, P.Y., and Sun, H.Q., Study on drought resistance of four native grass species, Pratacul. Sci., 2013, vol. 9, pp. 1386‒1393.

    Google Scholar 

  20. Li, C.H., Li, S.J., Zhang, J., and Sun, H.Q., Observations on spike differentiation of Kengyilia thoroldiana, Pratacul. Sci., 2013, vol. 8, pp. 1189‒1193.

    Google Scholar 

  21. Li, S.J., Li, C.H., and Sun, H.Q., Research progress and development prospect on Kengyilia thoroldiana, Pratacul. Sci., 2009, vol. 1, pp. 64‒68.

    Google Scholar 

  22. Lin, D., Xu, Q., Liu, Y., Wei, J., Qu, L., Gu, H., and Chen, Z.L., The antibacterial effect of the secreted peptide from Bacillus subtilis SO113 and separation and purification of the antibacterial peptides, Chin. J. Agric. Biotechnol., 2001, vol. 9, pp. 77‒80.

    Google Scholar 

  23. Lulai, E.C., Suttle, J.C., Olson, L.L., Neubauer, J.D., Campbell, L.G., and Campbell, M.A., Wounding induces changes in cytokinin and auxin content in potato tuber, but does not induce formation of gibberellins, J. Plant Physiol., 2015, vol. 192, pp. 22‒28.

    Google Scholar 

  24. Mena-Violante, H.G. and Olalde-Portugal, V., Alteration of tomato fruit quality by root inoculation with plant growth-promoting rhizobacteria (PGPR): Bacillus subtilis, BEB-13BS, Sci. Hortic., 2007, vol. 113, pp. 103‒106.

    Article  CAS  Google Scholar 

  25. Mizuguchi, Y., Fukunaga, M., and Taniguchi, H., Plasmid deoxyribonucleic acid and translucent-to-opaque variation in mycobacterium intracellulare 103, J. Bacteriol., 1981, vol. 146, pp. 656–659.

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Nahi, A., Othman, R., and Omar, D., Effects of SB16 bacterial strain and herbicides on endophytic bacterial populations and growth of aerobic rice, Plant, Soil Environ., 2016, vol. 62, pp. 453‒459.

    Article  CAS  Google Scholar 

  27. Ping, X., Jiang, Z., and Li, C., Status and future perspectives of energy consumption and its ecological impacts in the Qinghai–Tibet region, Renew. Sustain. Energy Rev., 2011, vol. 15, pp. 514‒523.

    Article  Google Scholar 

  28. Poopathi, S., and Kumar, K.A., Novel fermentation media for production of Bacillus thuringiensis subsp. israelensis., J. Econ. Entomol., 2003, vol. 96, pp. 1039‒1044.

    Article  CAS  PubMed  Google Scholar 

  29. Rehman, H.U., Siddique, N.N., Aman, A., Nawaz, M.A., Baloch, A.H., and Qader, S.A.U., Morphological and molecular based identification of pectinase producing Bacillus licheniformis, from rotten vegetable, J. Genet. Eng. Biotechnol., 2015, vol. 13, pp. 139‒144.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Ruizherrera, J., Leónramírez, C., Veranuñez, A., Sánchezarreguín, A., Ruizmedrano, R., and Salgadolugo, H., A novel intracellular nitrogen-fixing symbiosis made by ustilago maydis and Bacillus spp., New Phytol., 2015, vol. 207, pp. 769‒777.

    Article  CAS  Google Scholar 

  31. Santoyo, G., Moreno-Hagelsieb, G., Del, C.O.M., and Glick, B.R., Plant growth-promoting bacterial endophytes, Microbiol. Res., 2016, vol. 183, pp. 92‒99.

    Article  CAS  PubMed  Google Scholar 

  32. Shao, J., Xu, Z., Zhang, N., Shen, Q., and Zhang, R., Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens, SQR9, Biol. Fertil. Soils, 2015, vol. 51, pp. 321‒330.

    Article  CAS  Google Scholar 

  33. Shiau, Y.J. and Chu, C.Y., Comparative effects of ultrasonic transducers on medium chemical content in a nutrient mist plant bioreactor, Sci. Hortic., 2010, vol. 123, pp. 514‒520.

    Article  CAS  Google Scholar 

  34. Stoof, C.R., Richards, B.K., Woodbury, P.B., Fabio, E.S., Brumbach, A.R., and Cherney, J., Untapped potential: opportunities and challenges for sustainable bioenergy production from marginal lands in the northeast USA, BioEnergy Res., 2015, vol. 8, pp. 482‒501.

    Article  Google Scholar 

  35. Strauss, M.L.A., Jolly, N.P., Lambrechts, M.G., and Rensburg, P.V., Screening for the production of extracellular hydrolytic enzymes by non-saccharomyces wine yeasts, J. Appl. Microbiol., 2001, vol. 91, pp. 182‒190.

    Article  CAS  PubMed  Google Scholar 

  36. Voort, M.V.D., Meijer, H.J.G., Schmidt, Y., Watrous, J., Dekkers, E., Mendes, R., Dorrestein, P.C., Gross, H., and Raaijmakers, J.M., Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SHC 52 for antimicrobial compounds, Front. Microbiol., 2015, vol. 6, p. 693.

    Google Scholar 

  37. Xie, S., Wu, H., Zhang, H., Wu, L., Zhu, Q., and Gao, X., Plant growth promotion by spermidine-producing Bacillus subtilis OKB105, Mol. Plant Microbe Interact., 2014, vol. 27, pp. 655‒663.

    Article  CAS  PubMed  Google Scholar 

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ACKNOWLEDGMENTS

Authors are grateful to the authorities of respective departments for support in doing this research. This work was funded by the National Natural Science Foundation of China (31660543) and International Sci-Technology Cooperation Project of Qinghai Provincial Science and Technology Department (2018-HZ-813).

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Authors and Affiliations

  1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, PR China

    X. Wu & Y. Xie

  2. Department of Grassland Science, College of Agricultural and Husbandry, Qinghai University, Xining, PR China

    X. Wu, Y. Xie, Sh. Chai & L. Chen

  3. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China

    J. Qiao

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  1. X. Wu
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Correspondence to Y. Xie.

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Wu, X., Xie, Y., Qiao, J. et al. Rhizobacteria Strain from a Hypersaline Environment Promotes Plant Growth of Kengyilia thoroldiana. Microbiology 88, 220–231 (2019). https://doi.org/10.1134/S0026261719020127

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