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Methacrylate-Reducing Activity of Anaerobic Bacteria Anaeromyxobacter dehalogenans and Denitrovibrio acetiphilus

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Abstract—

Periplasmic methacrylate-reducing activity was shown for anaerobic acetate-oxidizing gram-negative bacteria Anaeromyxobacter dehalogenans 2CP-1Т (class Deltaproteobacteria) and Denitrovibrio acetiphilus DSM 12809Т (class Deferribacteres), both possessing homologous genes for methacrylate redox system components. No acrylate reductase activity was revealed in these bacteria. A. dehalogenans and D. acetiphilus were also found to reduce fumarate, exhibiting periplasmic and intracellular fumarate reductase activity, respectively. D. acetiphilus was found to possess nitrate reductase activity of periplasmic and intracellular localization. The possible correspondence between reductase activities and the hypothetical proteins genes in the known genomes of A. dehalogenans and D. acetiphilus are discussed.

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ACKNOWLEDGMENTS

This work was supported by the Russian Foundation for Basic Research, project no. 17-04-01068 “The Anaerobic Enzyme System, Reducing Methacrylate.” We are grateful to Prof. F.A. Kondrashov (Institute of Science and Technology, Austria) for his help in the acquisition of cultures from DSMZ.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia

    O. V. Arkhipova, E. N. Biryukova, T. N. Abashina, G. V. Khokhlova & V. V. Ashin

  2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, 142290, Pushchino, Russia

    G. V. Mikoulinskaia

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  1. O. V. Arkhipova
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  2. E. N. Biryukova
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  3. T. N. Abashina
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  4. G. V. Khokhlova
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  6. G. V. Mikoulinskaia
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Correspondence to O. V. Arkhipova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Babchenko

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Arkhipova, O.V., Biryukova, E.N., Abashina, T.N. et al. Methacrylate-Reducing Activity of Anaerobic Bacteria Anaeromyxobacter dehalogenans and Denitrovibrio acetiphilus. Microbiology 88, 137–145 (2019). https://doi.org/10.1134/S0026261719020024

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