Abstract
This is the first report on depicting the pioneering microbiota of Unnai hot spring using shotgun metagenome sequencing approach. Community analysis encompassed a total of 688,059 sequences with the total size 125.31 Mbp and 46% G + C content. Sequencing metagenome reported about 992 species belonged to 40 different phyla dominated by Firmicutes (97.49%), Proteobacteria (1.36%), and Actinobacteria (0.31%). In functional analysis, Non-Supervised Orthologous Groups (NOG) annotation revealed the predominance of poorly characterized reads (82.79%). Moreover, the subsystem classification displayed 19% genes assigned to carbohydrates metabolism, 12% genes allocated to clustering-based subsystems, 10% genes belonged to amino acids and its derivatives. The result suggests the huge bacterial diversity which will be useful for further characterizing the economically important bacteria for biotechnological applications.
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References
Apha, A.W.W.A., and W.E.F., Standard Methods for the Examination of Water and Waste Water, 2012, vol. 22.
Dudhagara, P., Bhavsar, S., Bhagat, C., Ghelani, A., Bhatt, S., and Patel, R., Web resources for metagenomics studies, Genomics, Proteomics Bioinformatics, 2015, vol. 13, no. 5, pp. 296–303. http://doi.org/10.1016/j.gpb. 2015.10.003.
Dudhagara, P., Bhavasar, S., Ghelani, A., and Bhatt, S., Isolation, characterization and investing the industrial applications of thermostable and solvent tolerant serine protease from Hot spring isolated thermophilic Bacillus licheniformis U1, Int. J. Appl. Sci. Biotechnol., 2014, vol. 2, no. 1, pp. 75–82. http://doi.org/10.3126/ijasbt.v2i1.9519.
Mangrola, A.V., Dudhagara, P., Koringa, P. Joshi, C.G., and Patel, R.K., Shotgun metagenomic sequencing based microbial diversity assessment of Lasundra hot spring, India, Genomics Data, 2015a, vol. 4, pp. 73–75. http://doi.org/10.1016/j.gdata.2015.03.005.
Mangrola, A., Dudhagara, P., Koringa, P. Joshi, C.G., Parmar, M., and Patel, R., Deciphering the microbiota of Tuwa hot spring, India using shotgun metagenomic sequencing approach, Genomics Data, 2015b, vol. 4, pp. 153–155. http://doi.org/10.1016/j.gdata.2015.04.014.
Meyer, F., Paarmann, D., D’Souza, M., Olson, R., Glass, E.M., Kubal, M., Paczian, T., Rodriguez, A., Stevens, R., Wilke, A., Wilkening, J., and Edwards, R.A., The metagenomics RAST server—a public resource for the automatic phylogenetic and functional analysis of metagenomes, BMC Bioinformatics, 2008, vol. 9, no. 1, p. 386. http://dx.doi.org/10.1186/1471-2105-9-386.
Nikolaki, S. and Tsiamis, G., Microbial diversity in the era of omic technologies, BioMed Res. Int., 2013, vol. 2013, pp. 1–15. http://doi.org/10.1155/2013/958719.
Patel, R., Mevada, V., Prajapati, D., Dudhagara, P., Koringa, P., and Joshi, C.G., Metagenomic sequence of saline desert microbiota from wild ass sanctuary, Little Rann of Kutch, Gujarat, India, Genomics Data, 2015, vol. 3, pp. 137–139. http://doi.org/10.1016/j.gdata.2015.01.003.
Sahoo, R.K., Gaur, M., Das, A., Singh, A., Kumar, M., and Subudhi, E., Comparative analysis of 16S rRNA gene Illumina sequence for microbial community structure in diverse unexplored hot springs of Odisha, India, Geomicrobiology Journal, 2016, vol. 0, pp. 1–10. http://doi.org/10.1080/01490451.2016.1238980.
Sharma, A., Paul, D., Dhotre, D., Jani, K., Pandey, A., and Shouche, Y.S., Deep sequencing analysis of bacterial community structure of Soldhar hot spring, India, Microbiology, 2017, vol. 86, pp. 136–142. doi 10.1134/S0026261717010118.
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Mangrola, A.V., Dudhagara, P.R., Koringa, P.G. et al. Metagenomic microbial community profiling of Unnai hot spring by Ion-Torrent based shotgun sequencing. Microbiology 87, 143–146 (2018). https://doi.org/10.1134/S0026261718010113
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DOI: https://doi.org/10.1134/S0026261718010113