Abstract
Bacterial diversity of four thermally different hot springs of Ratnagiri district, Maharashtra, India, was investigated using culture-dependent and culture-independent approaches. A total of 144 bacterial cultures were isolated and identified using MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) and 16S rRNA gene sequencing. Culture-independent analysis by Ion Torrent sequencing targeting the V3 region of the 16S rRNA gene revealed the predominance of Firmicutes across all the hot springs, followed by Chloroflexi, Bacteroidetes, Cyanobacteria, Proteobacteria, Armatimonadetes, Actinobacteria, Nitrospirae, Acidobacteria, and Deinococcus–Thermus, with subtle differences in their abundance. At the lower taxonomic rank of genus, we noted the prevalence of Acinetobacter followed by Clostridium, Planomicrobium, Bacillus, Streptomyces, and Leptolyngbya. Metagenomics imputation using in silico approach revealed divergence in the metabolic capabilities of bacterial communities along the thermal gradient of host springs, with site TS (63 °C) featuring the abundant functional gene families.
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Acknowledgements
We acknowledge Mr. Harsh Patel and Mr. Tanay Shetty for the hot springs sample collections. We also acknowledge Mr. Swapnil Kajale for his technical support. The authors are very thankful to DBT-NCCS, India, and Dr Yogesh Shouche for permission and accessibility of research facility to conduct the experiments performed in this study. The study was supported by the inhouse funding by the ‘Department of Biotechnology (DBT), Government of India’ (by Grant No. BT/Coord.II/01/03/2016), under the project Establishment of Centre for excellence National Centre for Microbial Resource (NCMR).
Funding
This work was funded by the Department of Biotechnology, Ministry of Science and Technology (Grant no. BT/Coord.II/01/03/2016).
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Mathan Kumar, R., Jani, K., Parvathi, J.R. et al. Bacterial diversity of geochemically distinct hot springs located in Maharashtra, India. Arch Microbiol 204, 110 (2022). https://doi.org/10.1007/s00203-021-02728-2
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DOI: https://doi.org/10.1007/s00203-021-02728-2