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Genetic markers for search of rhizobia based on symbiotic genes

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Abstract

The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, while more variable genes nifK and nodC could be used for their primary identification. Efficiency of the proposed method was shown in analysis of bacterial isolates obtained from Onobrychis arenaria and Astragalus cicer root nodules.

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Scientific Centre Russian Academy of Sciences, Ufa, Russia

    E. S. Akimova, R. S. Gumenko, Z. R. Vershinina, Al. Kh. Baymiev & An. Kh. Baymiev

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  1. E. S. Akimova
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  2. R. S. Gumenko
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  3. Z. R. Vershinina
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  4. Al. Kh. Baymiev
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  5. An. Kh. Baymiev
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Correspondence to An. Kh. Baymiev.

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Original Russian Text © E.S. Akimova, R.S. Gumenko, Z.R. Vershinina, Al.Kh. Baymiev, An.Kh. Baymiev, 2017, published in Mikrobiologiya, 2017, Vol. 86, No. 5, pp. 621–628.

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Akimova, E.S., Gumenko, R.S., Vershinina, Z.R. et al. Genetic markers for search of rhizobia based on symbiotic genes. Microbiology 86, 640–646 (2017). https://doi.org/10.1134/S0026261717050034

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  • DOI: https://doi.org/10.1134/S0026261717050034

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