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Proteomic analysis of sensitive and multi drug resistant Mycobacterium tuberculosis strains

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Abstract

Multidrug-resistant tuberculosis (MDR-TB) is caused by bacteria that are resistant to the most effective anti TB drugs (Isoniazid and Rifampicin) with or without resistance to other drugs. Novel intervention strategies to eliminate this disease based on finding proteins can be used for designing new drugs or new and reliable kits for diagnosis. The aim of this study was to compare the protein profile of MDR-TB with sensitive isolates. Two-dimensional gel electrophoresis (2DE) along with mass spectrometry is a powerful and effective tool to identification and characterization of Mycobacterium tuberculosis. Two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was used for diagnosis and comparison of proteins. We identified 14 protein spots in MDR-TB isolates that 2DE analysis showed these spots absent in M. tuberculosis sensitive isolates (Rv1876, Rv0379, Rv0147, Rv2031c, Rv3597c, Rv1886c, MT0493, Rv0440, Rv3614c, Rv1626, Rv0443, Rv0475, Rv3057 and unknown protein. The results showed 22 protein spots which were up regulated (or expressed) by the MDR-TB isolates, (Rv1240, Rv3028c, Rv2971, Rv2114c, Rv3311, Rv3699, Rv1023, Rv1308, Rv3774, Rv0831c, Rv2890c, Rv1392, Rv0719, Rv0054, Rv3418c, Rv0462, Rv2215, Rv2986c, Rv3248c and Rv1908c)). Two up regulated protein spots were identified in sensitive isolate (Rv1133c and Rv0685). These data will provide valuable clues in further investigation for suitable TB rapid tests or drug targets against drug resistant and sensitive of M. tuberculosis.

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Authors and Affiliations

  1. Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran

    Shamsi Yari, Alireza Hadizadeh Tasbiti, Mostafa Ghanei & Ahmadreza Bahrmand

  2. Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mostafa Ghanei

  3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Mohammad Ali Shokrgozar

  4. Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Behrouz Vaziri

  5. Molecular Medicine Dept, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Reza Mahdian

  6. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

    Fatemeh Yari

Authors
  1. Shamsi Yari
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  2. Alireza Hadizadeh Tasbiti
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  3. Mostafa Ghanei
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  4. Mohammad Ali Shokrgozar
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  5. Behrouz Vaziri
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  6. Reza Mahdian
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  7. Fatemeh Yari
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  8. Ahmadreza Bahrmand
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Correspondence to Ahmadreza Bahrmand.

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Yari, S., Tasbiti, A.H., Ghanei, M. et al. Proteomic analysis of sensitive and multi drug resistant Mycobacterium tuberculosis strains. Microbiology 85, 350–358 (2016). https://doi.org/10.1134/S0026261716030164

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  • DOI: https://doi.org/10.1134/S0026261716030164

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