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Comparative sensitivity of the luminescent Photobacterium phosphoreum, Escherichia coli, and Bacillus subtilis strains to toxic effects of carbon-based nanomaterials and metal nanoparticles

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Abstract

A comparative analysis of the four commercially available and laboratory luminescent sensor strains to the toxic effect of 10 carbon-based nanomaterials (CBNs) and 10 metal nanoparticles (MNPs) was carried out in this study. The bioluminescence inhibition assays with marine Photobacterium phosphoreum and recombinant Escherichia coli strains were varied in minimal toxic concentrations and EC50 values but led to well-correlated biotoxicity evaluation for the most active compounds, which were ranked as Cu > (MgO, CuO) > (fullerenol, graphene oxide). The novel sensor strain Bacillus subtilis EG168-1 exhibited the highest sensitivity to CBNs and MNPs, which increased significantly the number of toxic compounds causing the bacterial bioluminescence inhibition effect.

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Authors and Affiliations

  1. State Research Center for Dermatovenerology and Cosmetology, Moscow, Russia

    D. G. Deryabin

  2. Orenburg State University, Orenburg, Russia

    L. V. Efremova & I. F. Karimov

  3. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia

    I. V. Manukhov & E. Yu. Gnuchikh

  4. Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Russia

    I. V. Manukhov

  5. All-Russian Research Institute of Beef Cattle Breeding, Orenburg, Russia

    S. A. Miroshnikov

Authors
  1. D. G. Deryabin
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  2. L. V. Efremova
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  3. I. F. Karimov
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  4. I. V. Manukhov
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  5. E. Yu. Gnuchikh
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  6. S. A. Miroshnikov
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Correspondence to D. G. Deryabin.

Additional information

Original Russian Text © D.G. Deryabin, L.V. Efremova, I.F. Karimov, I.V. Manukhov, E.Yu. Gnuchikh, S.A. Miroshnikov, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 2, pp. 177–186.

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Deryabin, D.G., Efremova, L.V., Karimov, I.F. et al. Comparative sensitivity of the luminescent Photobacterium phosphoreum, Escherichia coli, and Bacillus subtilis strains to toxic effects of carbon-based nanomaterials and metal nanoparticles. Microbiology 85, 198–206 (2016). https://doi.org/10.1134/S0026261716020053

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  • DOI: https://doi.org/10.1134/S0026261716020053

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