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ATP pool and bioluminescence in psychrophilic bacteria Photobacterium phosphoreum

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Abstract

Bioluminescence activity and ATP pool were investigated in the cells of psychrophilic bacteria Photobacterium phosphoreum collected from the exponential and stationary growth phases and immobilized in polyvinyl alcohol (PVA) cryogel. In liquid culture, ATP pool remained at an almost constant level throughout the luminescence cycle (over 100 h). The ATP pool in the stationary-phase and PVA-immobilized cells remained constant throughout their incubation in the medium (over 200 h) and in 3% NaCl solution (over 100 h). Quantitative assessment of integral photon yield and ATP pool indicated that bioluminescence decay in growing or stationary cells was not caused by limitation from the energy substrates of the luciferase reaction. Kinetic and quantitative parameters of emission activity and ATP pool excluded the possibility of formation of the aldehyde substrate for luciferase via reduction of the relevant fatty acids in NADPH and ATP-dependent reductase reaction and its oxidation in the monooxygenase reaction. Our results indicate that the aliphatic aldehyde is not utilized in the process of light emission.

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References

  1. Hastings, J.W. and Nealson, K.H., Bacterial bioluminescence, Annu. Rev. Microbiol., 1977, vol. 31, pp. 549–595.

    Article  CAS  PubMed  Google Scholar 

  2. Meighen, E.A., Genetics of bacterial bioluminescence, Annu. Rev. Genet., 1994, vol. 28, pp. 117–139.

    Article  CAS  PubMed  Google Scholar 

  3. Nealson, K.H. and Hastings, J.W., Bacterial bioluminescence: its control and ecological significance, Microbiol. Rev., 1979, vol. 43, no. 4, pp. 406–518.

    Google Scholar 

  4. Ulitzur, S. and Hastings, J.M., Growth, luminescence, respiration, and the adenosintriphosphate pool in Beneckea harveyi, J. Bacteriol., 1978, vol. 133, pp. 1307–1313.

    CAS  PubMed Central  PubMed  Google Scholar 

  5. Ulitzur, S., The regulatory control of the bacterial luminescence system-a new view, J. Biolumin. Chemilumin., 1989, vol. 4, pp. 317–325.

    Article  CAS  PubMed  Google Scholar 

  6. Lee, B., Lee, J., Shin, D., and Kim, E., Statistical optimization of bioluminescence of Photobacterium phosphoreum KCTC 2852, J. Biosci. Bioeng., 2001, vol. 92, no. 1, pp. 72–76.

    CAS  PubMed  Google Scholar 

  7. Kuts, V.V. and Ismailov, A.D., Physiological and emission characteristics of the luminescent bacterium Photobacterium phosphoreum from the White Sea, Microbiology (Moscow), 2009, vol. 78, no. 5, pp. 554–558.

    Article  CAS  Google Scholar 

  8. Karl, D.M. and Nealson, K.H., Regulation of cellular methabolism during synthesis and expression of the luminous system in Beneckea and Photobacterium, J. Gen. Microbiol., 1980, vol. 117, pp. 357–368.

    CAS  Google Scholar 

  9. Efremenko, E.N., Sen’ko, O.V., Kuts, V.V., Alenina, K.A., Kholstov, A.V., and Ismailov, A.D., RF Patent 2394910, 2010.

  10. Makiguchi, N., Arita, M., and Asai, Y., Immobilisation of luminous bacterium and light intensity of luminous material, J. Ferment. Technol., 1980, vol. 58, no. 1, pp. 17–21.

    CAS  Google Scholar 

  11. Lozinsky, V.I. and Plieva, F.M., Poiy(vinyl alcohol) cryogels employed as matrices for cell immobilization, Enzyme Microb. Technol., 1998, vol. 23, pp. 227–242.

    Article  CAS  Google Scholar 

  12. Philp, J.C., Balmand, S., Hajto, E., Bailey, M.J., Wiles, S., Whiteley, A.S., Lilley, A.K., Hajto, J., and Dunbar, S.A., Whole cell immobilized biosensors for toxicity assessment of a wastewater treatment plant treating phenolics, Anal. Chim. Acta, 2003, vol. 487, pp. 61–74.

    Article  CAS  Google Scholar 

  13. Ismailov, A.D., Kutz, V.V., and Yefremenko, E.N., Factors affecting the stability of a light emission at PVA-immobilized cells of Photobacterium phosphoreum, J. Luminescence, 2010, vol. 25, pp. 166–167.

    Google Scholar 

  14. Hastings, J.W. and Weber, G., Total quantum flux of isotropic sources, J. Opt. Soc. Am., 1963, vol.53, no. 12, pp. 1410–1415.

    Article  CAS  Google Scholar 

  15. Lundin, A., Use of firefly luciferase in ATP-related assays of biomass, enzymes, and metabolites, Meth. Enzymol., 2000, Academic, pp. 346–370.

    Google Scholar 

  16. Watanabe, H., Mimura, M., Takimoto, A., and Nakamura, T., Luminescence and respiratory activities of Photobacterium phosphoreum, J. Biochem., 1975, vol. 77, no. 6, pp. 1147–1155.

    CAS  PubMed  Google Scholar 

  17. Meighen, E.A., Biosynthesis of aliphatic aldehydes for the bacterial bioluminescent reaction stimulation by ATP and NADPH, Biochem. Biophys. Res. Commun., 1979, vol. 87, no. 4, pp. 1080–1086.

    Article  CAS  PubMed  Google Scholar 

  18. Watanabe, T. and Nacamura, T., Bioluminescence and cell growth of Photobacterium phosphoreum, J. Biochem., 1980, vol. 88, no. 3, pp. 815–817.

    CAS  PubMed  Google Scholar 

  19. Watanabe, H. and Hastings, J.W., Expression of bacterial luminescence is stimulated by nalidixic acid in a nalidixic acid resistant mutant, Arch. Microbiol., 1990, vol. 154, pp. 239–243.

    Article  CAS  Google Scholar 

  20. Ulitzur, S., Reinhertz, A, and Hastings, J.W., Factor affecting the cellular expression of bacterial luciferase, Arch. Microbiol., 1981, vol. 129, no. 1, pp. 67–71.

    Article  CAS  PubMed  Google Scholar 

  21. Shimomura, O., Bioluminescence: Chemical Principles and Methods, New Jersey: World Sci. Pub., 2008.

    Google Scholar 

  22. Shimomura, O., Johnson, F.H., and Morise, H., The aldehyde content of luminous bacteria and of an “aldehydeless” dark mutant, Proc. Natl. Acad. Sci. U. S. A., 1974, vol. 71, no. 12, pp. 4666–4668.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Rees, J.F., Wergifosse, B., Noiset, O., Dubuisson, V., Jassens, B., and Thompson, E.M., The origins of marine bioluminescence: turning oxygen defence mechanisms into deep-sea communication tools, J. Exp. Biol., 1998, vol. 201, pp. 1211–1221.

    CAS  PubMed  Google Scholar 

  24. Eley, M. and Cormier, M.J., On the function of aldehyde in bacterial bioluminescenc: evidence for the aldehyde reouirement during luminescence from the frozen state, Biochem. Biophys. Res. Commun., 1968, vol. 32, no. 3, pp. 454–460.

    Article  CAS  PubMed  Google Scholar 

  25. Karatani, H., Konaka, T., and Katsukawa, Ch., Properties of the bimodal fluorescent protein produced by Photobacterium phosphoreum, Photochem. Photobiol., 2000, vol. 71, no. 2, pp. 230–236.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia

    L. E. Aleskerova, K. A. Alenina, M. M. Mazhul’, N. A. Piskunkova & A. D. Ismailov

  2. Department of Chemical Enzymology, Chemical Faculty, Lomonosov Moscow State University, Moscow, Russia

    E. N. Efremenko

Authors
  1. L. E. Aleskerova
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  2. K. A. Alenina
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  3. E. N. Efremenko
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  4. M. M. Mazhul’
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  5. N. A. Piskunkova
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  6. A. D. Ismailov
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Correspondence to A. D. Ismailov.

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Original Russian Text © L.E. Aleskerova, K.A. Alenina, E.N. Efremenko, M.M. Mazhul’, N.A. Piskunkova, A.D. Ismailov, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 4, pp. 403–410.

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Aleskerova, L.E., Alenina, K.A., Efremenko, E.N. et al. ATP pool and bioluminescence in psychrophilic bacteria Photobacterium phosphoreum . Microbiology 83, 315–321 (2014). https://doi.org/10.1134/S0026261714040031

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