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Production of extracellular H2O2 and L-lysine-α-oxidase during bulk growth of the fungus Trichoderma cf. aureoviride Rifai VKM F-4268D under salt stress

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Abstract

In this article, we report on Some physiological aspects of the synthesis of extracellular L-lysine-α-oxidase (LO) by the fungus Trichoderma cf. aureoviride Rifai VKM F-4268D under salt stress conditions and discuss the possible role of this enzyme for the producer. It has been shown that The synthesis of extracellular LO and proteolytic enzymes is induced in the fungus T. cf. aureoviride Rifai VKM F-4268D during submerged cultivation on wheat bran under salt stress. It has been shown that LO biosynthesis is accompanied by H2O2 accumulation in the growth medium. It seems that the extracellular LO synthesis followed by hydrogen peroxide production under stress conditions provides an adaptive advantage for the producer fungus in its competition with other organisms.

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References

  1. Murthy, S.N. and Janardanasarma, M.K., Identification of L-Amino Acid/L-Lysine Alpha-Amino Oxidase in Mouse Brain, Mol. Cell. Biochem., 1999, vol. 197, no. 1–2, pp. 13–23.

    Article  PubMed  CAS  Google Scholar 

  2. Kitani, Y., Tsukamoto, C., Zhang, G., Nagai, H., Ishida, M., Ishizaki, S., Shimakura, K., Shiomi, K., and Nagashima, Y., Identification of an Antibacterial Protein as L-Amino Acid Oxidase in the Skin Mucus of Rockfish Sebastes schlegeli, FEBS J., 2007, vol. 274, no. 1, pp. 125–136.

    Article  PubMed  CAS  Google Scholar 

  3. Yamada, R., Ujiie, H., Kera, Y., Kitagawa, K., Arimoto, K., Takahashi, M., and Matsumura, Y., Purification and Properties of D-Aspartate Oxidase from Cryptococcus humicolus UJ1, Biochim. Biophys. Acta, 1996, vol. 1294, pp. 153–158.

    Article  PubMed  Google Scholar 

  4. Calderon, J., Olvera, L., Martinez, L.M., and Davila, G., A Neurospora crassa Mutant Altered in the Regulation of L-Amino Acid Oxidase, Microbiolology (UK), 1997, vol. 143, pp. 1969–1974.

    Article  CAS  Google Scholar 

  5. Sikora, L. and Murzluf, G.A., Regulation of L-Amino Acid Oxidase and of D-Amino Acid Oxidase in Neurospora crassa, Mol. Gen., 1982, vol. 186, no. 1, pp. 33–39.

    Article  CAS  Google Scholar 

  6. Kusakabe, H., Kodama, K., Kuninaka, A., Yoshino, H., Misono, H., and Soda, K., A New Antitumor Enzyme, L-Lysine Alpha-Oxidase from Trichoderma viride. Purification and Enzymological Properties, J. Biol. Chem., 1980, vol. 255, no. 3, pp. 976–981.

    PubMed  CAS  Google Scholar 

  7. Kusakabe, H., Kodama, K., Kuninaka, A., Yoshino, H., and Soda, K., Extracellular Production L-Lysine α-Oxidase in Wheat Bran Culture of a Strain of Trichoderma viride, Agric. Biol. Chem., 1979, vol. 43, no. 12, pp. 2531–2535.

    Article  CAS  Google Scholar 

  8. Kusakabe, H., Sugi, M., Kodamo, A., Kuninaka, A., Yoshino, H., and Soda, K., Antibacterial Activity of L-Lysine α-Oxidase against Rec+ and Rec− Strans of Bacillus subtilis, Agric. Biol. Chem., 1979, vol. 43, no. 6, pp. 1371–373.

    Article  CAS  Google Scholar 

  9. Makrushin, K.V., Arinbasarova, A.Yu., Lukasheva, E.V., Medentsev, A.G., and Berezov, T.T., Search for Fungal Producers of L-Lysine-α-Oxidase, Biotekhnologiya, 2011, no. 5, pp. 44–49.

  10. Gomez, D., Lucas-Elio, P., Sanchez-Amat, A., and Solano, F., A Novel Type of Lysine Oxidase: L-Lysine-Epsilon-Oxidase, Biochim. Biophys. Acta, 2006, vol. 1764, no. 10, pp. 1577–1585.

    Article  PubMed  CAS  Google Scholar 

  11. Lucas-Elio, P., Gomez, D., Solano, F., and Sanchez-Amat, A., The Antimicrobial Activity of Marinocine, Synthesized by Marinomonas mediterranea, Is due to Hydrogen Peroxide Generated by Its Lysine Oxidase Activity, J. Bacteriol., 2006, vol. 188, no. 7, pp. 2493–2501.

    Article  PubMed  CAS  Google Scholar 

  12. Böhmer, A., Müller, A., Passarge, M., Liebs, P., Honeck, H., and Müller, H., A Novel L-Glutamate Oxidase from Streptomyces endus. Purification and Properties, Eur. J. Biochem., 1989, vol. 182, no. 2, pp. 327–332.

    Article  PubMed  Google Scholar 

  13. Koyama, H., Purification and Characterization of a Novel L-Phenylalanine Oxidase (Deaminating and Decarboxylating) from Pseudomonas sp. P-501, J. Biochem., 1982, vol. 92, pp. 1235–1240.

    PubMed  CAS  Google Scholar 

  14. Lukasheva, E.V. and Berezov, T.T., L-Lysine-α-Oxidase: Physicochemical and Biological Properties, Biochemistry (Moscow), 2002, vol. 67, no. 10, pp. 1152–1158.

    Article  CAS  Google Scholar 

  15. Berezov, T.T., Molecular and Biochemical Bases of Enzyme Therapy of Tumors, Biomed. Khim., 2005, vol. 51, no. 3, pp. 235–247.

    PubMed  CAS  Google Scholar 

  16. Makrushin, K.V., Arinbasarova, A.Yu., Lukasheva, E.V., Medentsev, A.G., and Berezov, T.T., Biosynthesis of L-Lysine-α-Oxidase by Trichoderma Fungi, Tez. Dokl. VI Mosk. Mezhdunar. kongr.: “Biotekhnologiya: sostoyanie i perspektivy razvitiya” (Proc. 6th Moscow Int. Congr. “Biotechnology: State and Prospects”), Moscow, 2009.

  17. Esders, T.W. and Schubert, R.M., US Patent, no. 4166763, 1979.

  18. Rowan, A.D. and Buttle, D.J., Pineapple Cysteine Endopeptidases, Meth. Enzymol., 1994, vol. 244, pp. 555–568.

    Article  PubMed  CAS  Google Scholar 

  19. Mai-Prochnow, A., Lucas-Elio, P., Egan, S., Webb, J.S., Sanchez-Amat, A., and Kjelleberg, S., Hydrogen Peroxide Linked to Lysine Oxidase Activity Facilitates Biofilm Differentiation and Dispersal in Several Gram-Negative Bacteria, J. Bacteriol., 2008, vol. 190, no. 15, pp. 5493–5501.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    A. Yu. Arinbasarova, K. V. Makrushin & A. G. Medentsev

  2. Peoples’ Friendship University of Russia, ul. Miklukho-Maklaya 6, Moscow, 117198, Russia

    E. V. Lukasheva & T. T. Berezov

Authors
  1. A. Yu. Arinbasarova
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  2. K. V. Makrushin
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  3. A. G. Medentsev
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  4. E. V. Lukasheva
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  5. T. T. Berezov
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Corresponding author

Correspondence to A. Yu. Arinbasarova.

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Original Russian Text © A.Yu. Arinbasarova, K.V. Makrushin, A.G. Medentsev, E.V. Lukasheva, T.T. Berezov, 2012, published in Mikrobiologiya, 2012, Vol. 81, No. 6, pp. 741–744.

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Arinbasarova, A.Y., Makrushin, K.V., Medentsev, A.G. et al. Production of extracellular H2O2 and L-lysine-α-oxidase during bulk growth of the fungus Trichoderma cf. aureoviride Rifai VKM F-4268D under salt stress. Microbiology 81, 684–687 (2012). https://doi.org/10.1134/S0026261712050025

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  • DOI: https://doi.org/10.1134/S0026261712050025

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