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The expression, secretion and activity of the aspartic protease MpAPr1 in Metschnikowia pulcherrima IWBT Y1123

  • Genetics and Molecular Biology of Industrial Organisms - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Protease-secreting yeasts have broad biotechnological potential for application to various industrial processes, including winemaking. However, this activity is influenced by the yeast response to environmental factors such as nitrogen and protein sources, as are found in grape juice. In this study, the wine-relevant yeast Metschnikowia pulcherrima IWBT Y1123, with known protease-secreting ability, was subjected to different nitrogen-containing compounds to monitor their impact on protease secretion and activity. Protease activity increased above basal levels for haemoglobin-containing treatments, indicating an inductive influence of proteins. On the other hand, treatments containing both haemoglobin and assimilable nitrogen sources led to a delayed increase in protease activity and protein degradation, suggesting a nitrogen catabolite repression mechanism at work. Protease activity and expression were furthermore evaluated in grape juice, which revealed increased expression and activity levels over time as promising results for further investigations into the impact of this yeast on wine properties.

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Acknowledgements

This work is based on research supported in part by the National Research Foundation of South Africa (Grant number: 113303) and Winetech for their financial support.

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  1. Department of Viticulture and Oenology, Institute for Wine Biotechnology, Private Bag X1, Matieland, 7602, South Africa

    C. Snyman, L. W. Theron & B. Divol

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  1. C. Snyman
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  2. L. W. Theron
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  3. B. Divol
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Correspondence to B. Divol.

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Snyman, C., Theron, L.W. & Divol, B. The expression, secretion and activity of the aspartic protease MpAPr1 in Metschnikowia pulcherrima IWBT Y1123. J Ind Microbiol Biotechnol 46, 1733–1743 (2019). https://doi.org/10.1007/s10295-019-02227-w

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  • DOI: https://doi.org/10.1007/s10295-019-02227-w

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