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Ectothiorhodospira magna sp. nov., a new large alkaliphilic purple sulfur bacterium

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Abstract

Two strains of purple sulfur bacteria of the family Ectothiorhodospiraceae were isolated from moderately saline steppe lakes (with pH above 9.0) of the Transbaikal region (strain B7-7) and Mongolia (strain M10). The cells of the novel strains were spiral-shaped, 2.0–3.2 × 9.6–20.0 μm, motile due to a polar tuft of flagella. Photosynthetic pigments were represented by bacteriochlorophyll a and carotenoids of the spirilloxanthin series. Photosynthetic membranes were represented by long strands of lamellae distributed throughout the whole cell; unlike most Ectothiorhodospiraceae species, the membranes were not packed into regular stacks. Bacteria were capable of weak growth on sulfide and slow grow on hydrogen under photoautotrophic conditions. The best growth was noted on sulfide in the presence of acetate and bicarbonate. Thiosulfate did not stimulate phototrophic growth, even in the presence of organic substrates. The new isolates were alkaliphiles growing at a pH optimum of 9–10. Growth was possible within a salinity range of 0–80 g/l NaCl, with an optimum at 5–15 g/l NaCl. The morphology, the structure of the photosynthetic apparatus (strands of lamellae), and the physiology of the new strains were similar to those of Thiorhodospira sibirica. However, analysis of the 16S rRNA gene sequences demonstrated that the studied isolates were closely related to the type strain Ectothiorhodospira shaposhnikovii (99% similarity) of the family Ectothiorhodospiraceae, whereas the level of similarity between the new strains and Thiorhodospira sibirica was only 94–95%. According to the results of DNA-DNA hybridization, the DNA-DNA homology level between the tested strains was almost 100%; the similarity between the new isolates and the type strain Ectothiorhodospira shaposhnikovii was only 58%. The isolates differed from other representatives of the genus Ectothiorhodospira in the structure of the gene encoding the key enzyme of autotrophic CO2 fixation, ribulose-1,5-bisphosphate carboxylase (RuBisCo), which was similar to the RuBisCo genes of members of another family of sulfur bacteria, Chromatiaceae. The new isolates of purple bacteria were described as a new species of the genus Ectothiorhodospira, Ect. magna sp. nov. with the type strain B7-7T (= VKM B-2537 = DSM 22250).

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    I. A. Bryantseva, T. P. Tourova, N. A. Kostrikina & V. M. Gorlenko

  2. Moscow State University, Moscow, 119992, Russia

    O. L. Kovaleva

Authors
  1. I. A. Bryantseva
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  2. T. P. Tourova
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  3. O. L. Kovaleva
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  4. N. A. Kostrikina
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  5. V. M. Gorlenko
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Correspondence to V. M. Gorlenko.

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Original Russian Text © I.A. Bryantseva, T.P. Tourova, O.L. Kovaleva, N.A. Kostrikina, V.M. Gorlenko, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 6, pp. 782–792.

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Bryantseva, I.A., Tourova, T.P., Kovaleva, O.L. et al. Ectothiorhodospira magna sp. nov., a new large alkaliphilic purple sulfur bacterium. Microbiology 79, 780–790 (2010). https://doi.org/10.1134/S002626171006010X

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  • DOI: https://doi.org/10.1134/S002626171006010X

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