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Analysis of Azospirillum brasilense plasmid loci coding for (Lipo)polysaccharides synthesis enzymes

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Abstract

Lipopolysaccharides LpsI and LpsII containing the same O-specific polysaccharide (OPS), yet different in antigenic structure and charge, have been revealed in the rhizobacterium Azospirillum brasilense Sp245. In the present work, four putative glycosyltransferase genes were identified in a 14-kbp fragment of a 120-MDa plasmid of Sp245, p120-lpsKM348X. Insertional mutagenesis of one of them, encoding the predicted ADP-heptose:LPS-heptosyltransferase, resulted in LpsI loss. By means of DNA hybridizations and PCR with primers specific towards several sites of p120-lpsKM348X, it was demonstrated that homologous segments of 120-MDa plasmids of A. brasilense strains Sp245 and Sp107, which are characterized by identical structures of the OPS repeating units, are practically identically organized. In an 85-MDa plasmid of Sp245, a locus was identified with high homology to the plasmid genes of glycosyltransferases and conserved membrane-bound proteins from a wide range of soil bacteria.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciencest, pr. Entuziastov 13, Saratov, 410049, Russia

    E. I. Katsy, L. P. Petrova & O. V. Kulibyakina

  2. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia

    A. G. Prilipov

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  1. E. I. Katsy
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  2. L. P. Petrova
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  3. O. V. Kulibyakina
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  4. A. G. Prilipov
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Original Russian Text © E.I. Katsy, L.P. Petrova, O.V. Kulibyakina, A.G. Prilipov, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 2, pp. 239–245.

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Katsy, E.I., Petrova, L.P., Kulibyakina, O.V. et al. Analysis of Azospirillum brasilense plasmid loci coding for (Lipo)polysaccharides synthesis enzymes. Microbiology 79, 216–222 (2010). https://doi.org/10.1134/S002626171002013X

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