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The strategy of strain selection for a mixed culture performing rapid conversion of a mixture of polyaromatic compounds

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Abstract

The strain Sphingomonas sp. VKM V-2434 converts the mixture of seven polyaromatic compounds (PACs): fluorene, dibenzothiophene, carbazole, phenanthrene, anthracene, fluoranthene, and pyrene. The effect of each of the above PACs on the rate of mixture conversion was determined. The following two strains, which utilize the substances inhibiting the studied process, were added to the culture: strain FON-11 utilizing 9-fluorenone (fluorene metabolite) and strain CBZ-21 utilizing carbazole. In the case of the mixed culture of three strains, conversion rates were 1.5 and 1.2–3.8 times higher for the PAC mixture and its individual components, respectively, than the rates for Sphingomonas sp. VKM V-2434 monoculture. The degree of degradation of PAC conversion products increased from 32 to 44%. The rate of PAC conversion by the mixed culture exceeded the sum of conversion rates for the individual component strains; this cooperative effect was particularly marked for anthracene and pyrene.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    M. A. Baboshin & L. A. Golovleva

Authors
  1. M. A. Baboshin
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  2. L. A. Golovleva
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Correspondence to M. A. Baboshin.

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Original Russian Text © M.A. Baboshin, L.A. Golovleva, 2010, published in Mikrobiologiya, 2010, Vol. 79, No. 1, pp. 79–88.

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Baboshin, M.A., Golovleva, L.A. The strategy of strain selection for a mixed culture performing rapid conversion of a mixture of polyaromatic compounds. Microbiology 79, 73–82 (2010). https://doi.org/10.1134/S0026261710010108

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