Abstract
Six bacterial strains capable of using, as sole carbon and energy source, at least one of the following polycyclic aromatic hydrocarbons (PAH), naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene, were isolated. The interactions between these PAH during their biodegradation were studied in experiments involving PAH pairs, one PAH at least being used as a carbon source. All individual strains were found capable of cometabolic degradation of PAH in a range varying among strains. Inhibition phenomena, sometimes drastic, were often observed but synergistic interactions were also detected. Naphthalene was toxic to all strains not isolated on this compound. Strain associations were found efficient in relieving inhibition phenomena, including the toxic effect of naphthalene. Accumulation of water-soluble metabolites was consistently observed during PAH degradation.
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References
Arvin E, Jensen B, Torp Gundersen A (1989) Substrate interactions during aerobic biodegradation of benzene. Appl Environ Microbiol 55:3221–3225
Barbieri P, Palladino L, Di Gennaro P, Galli E (1993) Alternative pathways for o-xylene or m-xylene and p-xylene degradation in a Pseudomonas stutzeri strain. Biodegradation 4:71–80
Boldwin B, Tiehm A, Fritzsche C (1993) Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp. Appl Environ Microbiol 59:1927–1930
Cerniglia CE (1992) Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3:351–368
Cerniglia CE (1993) Biodegradation of polycyclic aromatic hydrocarbons. Curr Opin Biotechnol 4:331–338
Chang MK, Voice TC, Criddle CS (1993) Kinetics of competitive inhibition and cometabolism in the biodegradation of benzene, toluene and p-xylene by two Pseudomonas isolates. Biotechnol Bioeng 41:1057–1065
Gibson DT, Subramanian V (1984) Microbial degradation of aromatic hydrocarbons. In: Gibson DT (ed) Microbial degradation of organic compounds. Dekker, New York Basel, pp 181–252
Grifoll M, Casellas M, Bayona JM, Solanas AM (1992) Isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission products. Appl Environ Microbiol 58:2910–2917
Hegeman GD (1966) Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J Bacteriol 91:1140–1154
Heitkamp MA, Cerniglia CE (1988) Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. Appl Environ Microbiol 54:1612–1614
Horvath RS (1972) Microbial co-metabolism and the degradation of organic compounds in nature. Bacteriol Rev 36:146–155
Kelley I, Cerniglia CE (1991) The metabolism of fluoranthene by a species of Mycobacterium. J Ind Microbiol 7:19–26
Kelly I, Freeman JP, Evans FE Cerniglia CE (1993) Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1. Appl Environ Microbiol 59:800–806
Kiyohara H, Takizawa N, Nagao T (1992) Natural distribution of bacteria metabolizing many kinds of polycyclic aromatic hydrocarbons. J Ferment Bioeng 74:49–51
Kramers PGN, Heijden CA van der (1990) Polycyclic aromatic hydrocarbons (PAH): carcinogenicity data and risk extrapolations. In: Rose J (ed) Environmental topics, vol 1. Gordon and Breach, New York London Paris, pp 47–57
Monna L, Omori T, Kodama T (1993) Microbial degradation of dibenzofuran, fluorene, and dibenzo-p-dioxin by Staphylococus auriculans DBF63. Appl Environ Microbiol 59:285–289
Mueller JG, Chapman PJ, Blattmann BO, Pritchard PH (1990) Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis. Appl Environ Microbiol 56:1079–1086
Perry JJ (1979) Microbial cooxidation involving hydrocarbons. Microbiol Rev 43:59–72
Rosenfeld H, Feigelson P (1969) Synergistic and product induction of the enzymes of tryptophan metabolism in Pseudomonas acidovorans. J Bacteriol 97:697–704
Van Eyk J, Bartels TJ (1968) Paraffin oxidation in Pseudomonas aeruginosa. I. Induction of paraffin oxidation. J Bacteriol 96:706–712
Walter U, Beyer M, Klein J, Rehm HJ (1991) Degradation of pyrene by Rhodococcus sp. UW 1. Appl Microbiol Biotechnol 34:671–676
Weissenfels WD, Beyer M, Klein J (1990) Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures. Appl Microbiol Biotechnol 32:479–484
Weissenfels WD, Beyer M, Klein J, Rehm HJ (1991) Microbial metabolism of fluoranthene: isolation and identification of ring fission products. Appl Microbiol Biotechnol 34:528–535
Wilson SC, Jones KC (1993) Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): a review. Environ Pollut 81:229–249
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Bouchez, M., Blanchet, D. & Vandecasteele, JP. Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism. Appl Microbiol Biotechnol 43, 156–164 (1995). https://doi.org/10.1007/BF00170638
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DOI: https://doi.org/10.1007/BF00170638