Abstract
The amount and composition of fatty acids in the fungus Stilbella aciculosa associated with the marine macroorganism Apostichopus japonica (trepang) were determined by gas-liquid chromatography and gas chromatography-mass spectrometry. In the culture liquid of S. aciculosa, prostaglandins (PG) of groups E and F were revealed by UV spectroscopy. This finding was confirmed by the presence of direct precursors of PG, polyunsaturated eicosapentaenoic and docosahexaenoic acids, in the culture liquid. The biomass of this fungus contained PG of group B.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Eroshin, V.K., Dedyukhini, E.G., Satrutdinov, A.D., and Chistyakova, T.I., Growth-Coupled Lipid Synthesis in Mortierella alpina LPM 301, a Producer of Arachidonic Acid, Mikrobiologiya, 2002, vol. 71, no. 2, pp. 200–204 [Microbiology (Engl. Transl.), vol. 71, no. 2, pp. 169–172].
Vali, S.R., Sheng, H.Y., and Ju, Yi-H., An Efficient Method for the Purification of Arachidonic Acid from Fungal Single-Cell Oil (ARASCO), J. Am. Oil Chem. Soc., 2003, vol. 80, no. 7, pp. 725–730.
Van Dyk, M.S., Kock, J.-L.F., and Botha, A., Hydroxy Long-Chain Fatty Acids in Fungi, J. Microbiol. Biotechnol., 1994, vol. 10, no. 5, pp. 495–504.
Mitscher, L.A., Clark, G.W., and Hudson, P.B., Terrein, An Optically Active Prostaglandin Synthon of Fungal Origin. II. Chemical Conversion To 4(R)-Acetoxy-2-Cyclopentenone, Tetrahedron Lett., 1978, no. 29, pp. 2553–2556.
Zeleneva, R.N., Ustynyuk, T.K., Bragintseva, L.M., Polin, A.N., and Kovalenko, V.A., Fungal strain Fusarium sambucinum, a Prostaglandin Producer, RF Patent No. 1446928, Byull. Izobret., 1994, no. 24.
Tsitsigiannis, D.I., Kowieski, T.M., Zarnovski, R., and Keller, N.P., Three Putative Oxylipin Biosynthetic Genes Integrate Sexual and Asexual Development in Aspergillus nidulans, Microbiology (UK), 2005, vol. 151, pp. 1809–1821.
Lamačka, M., Šajbidor, J., The Occurrence of Prostaglandins and Related Compounds in Lower Organisms, Prostagl. Leukotr. Essent. Fatty Acids, 1995, vol. 52, pp. 357–364.
Feofilova, E.P., Heterothallism of Mucoraceous Fungi: A Review of Biological Implications and Uses in Biotechnology, Prikl. Biokhim. Mikrobiol., 2006, vol. 42, no. 5, pp. 501–519 [Appl. Biochem. Microbiol. (Engl. Transl.), vol. 42, no., 5, pp. 439–454].
Mischenko, T.Ya., Korotchenko, O.D., and Isai, S.V., Search for Compounds of Prostaglandine-Type Action in Marine Organisms, Byull. Eksperim. Biologii I Meditsiny, 1982, vol. 94, no. 11, pp. 94–96.
Bilai, V.I., Metody eksperimental’noi mikologii. Spravochnik: (Handbook on Methods in Experimental Mycology), Kiev: Naukova dumka, 1982.
Semenov, S.M., Laboratornye sredy dlya aktinomitsetov i gribov. Spravochnik (Handbook on Laboratory Media for Actinomycetes and Fungi), Moscow: Agropromizdat, 1990.
Korotchenko, O.D., Mischenko, T.Ya., and Isay, S.V., Prostaglandins of Japan Sea Invertebrates in Echinoderms, Comp. Biochem. Physiol., 1983, vol. 74C, no. 1, pp. 85–88.
Hartman, L., Lago, C.A., and Regino, N., The Quick Method of Preparation of Methyl Ester Fatty Acids from Lipids, Lab. Pract., 1973, vol. 22, pp. 475–476.
Weisberger, A., Elucidation of Organic Structures by Physical and Chemical Methods, Bentley, K.W., Ed., New York: Wiley, 1963 [Rus. Transl. Moscow: Khimiya, 1967, vol. 1].
Perera, S.K. and Fedor, L.R., Acid-and Base-Catalyzed Dehydration of Prostaglandin E2 To Prostaglandin A2 and General-Base-Catalyzed Isomerization of Prostaglandin A2 To Prostaglandin B2, J. Am. Chem. Soc., 1979, vol. 101, no. 24, pp. 7390–7393.
Ruban, E.L., Mikrobnye lipidy i lipazy (Microbial Lipids and Lipases), Moscow: Nauka, 1977.
Kawashima, H., Kamada, N., Sakuradani, E., Jareonkitmongkol, S., Akimoto, K., and Shimizu, S., Production of 8,11,14,17-cis-Eicosatetraenoic Acid by Δ5 Desaturase-Defective Mutants of an Arachidonic Acid-Producing Fungus, Mortierella alpina, J. Am. Oil Chem. Soc., 1997, vol. 74, no. 4, pp. 455–459.
Wierzbicki, A.S., Lloyd, M.D., Schofield, C.J., Feher, M.D., and Gibberd, F.B., Refsum’s Disease: a Peroxisomal Disorder Affecting Phytanic Acid α-Oxidation, J. Neurochem., 2002, vol. 80, no. 5, pp. 727–735.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © T.V. Kafanova, N.G. Busarova, Yu.V. Khudyakova, S.V. Isai, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 4, pp. 508–511.
Rights and permissions
About this article
Cite this article
Kafanova, T.V., Busarova, N.G., Khudyakova, Y.V. et al. Marine fungus Stilbella aciculosa as a potential producer of prostaglandins. Microbiology 77, 451–454 (2008). https://doi.org/10.1134/S0026261708040115
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261708040115