Abstract
This review discusses the prospects of using the potential of microorganisms for bioremediation of PCB-contaminated natural environments (soil, sediments, and sewage sludge) under anaerobic and aerobic conditions. A detailed analysis of the research conditions of original works has shown that the efficiency of bioremediation of PCB-contaminated matrices strongly depends on the character and degree of contamination. In the case of aerobic bioremediation, the best results were obtained with moderately contaminated soils and sediments (20 to 700 PCB/kg), in which the level of contamination decreased by 40–75%. These results could be achieved by repeated inoculation of a consortium of specific microorganisms (isolated or engineered) with concurrent addition of biphenyl as an inducer and of biosurfactants; their effect increased in a slurry bioreactor. PCB concentration decreased mainly due to the degradation of congeners with one to three chlorine atoms. The content of higher-chlorinated PCB can be noticeably decreased only under sequential anaerobic/aerobic treatment; the best effect was achieved with anaerobic granules. However, only in individual cases, mainly in laboratory experiments with freshly spiked PCB at moderate concentrations, was it possible to reduce their content to a level permissible for technogenic soils. The review begins with the description of the main metabolic pathways and patterns of biodegradation of these pollutants in natural and artificial environments.
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Borja, J., Taleon, D.M., Auresenia, J., and Gallardo, S., Polychlorinated Biphenyls and Their Biodegradation, Process. Biochemistry, 2005, vol. 40, pp. 1999–2013.
Fedorov, L.A., Dioxins as a Fundamental Factor of Technogenic Pollution, Zh. Ekologich. Khimii, 1993, no. 3, pp. 169–187.
Chavez, F.P., Gordillo, F., and Jerez, C.A., Adaptive Responses and Cellular Behavior of Biphenyl-Degrading Bacteria Toward Polychlorinated Biphenyls, Biotechnol. Adv., 2006, vol. 24, pp. 309–320.
Ross, G., The Public Health Implications of Polychlorinated Biphenyls (PCBs) in the Environment, Rev. Ecotox. Environ. Safety, 2004, vol. 59, pp. 275–291.
Abraham, W.R., Nogales, B., Golyshin, P.N., Pieper, D.H., and Timmis, K.N., Polychlorinated Biphenyl-Degrading Microbial Communities in Soils and Sediments, Curr. Opin. Microbiol., 2002, vol. 5, pp. 246–253.
De, S., Perkins, M., and Dutta, S.K., Nitrate Reductase Gene Involvement in Hexachlorobiphenyl Dechlorination by Phanerochaete chrysosporium, J. Hazard. Mater., 2006, vol. 135, pp. 350–354.
Mackova, M., Barriault, D., Francova, K., Sylvestre, M., Moder, M., Vrchotova, B., Lovecka, P., Najmanova, J., Demnerova, K., Novakova, M., Rezek, J., and Macek, T., Phytoremediation of Polychlorinated Biphenyls, Phytoremediation and Rhizoremediation, Mackova M. et al., Eds., Springer, 2006.
Bakker, D.J., De Vries, W., Vad de Plassche, E.J., and Van Pul, W.A.J., Manual for Performing Risk Assessments for Persistent Organic Pollutants in Aquatic Ecosystems. Guidelines for Critical Limits, Calculation Methods and Input Data, TNO-Report, TNO-MEPR98/376.
Ohtsubo, Y., Kudo, T., Tsuda, M., and Nagata, Y., Strategies for Bioremediation of Polychlorinated Biphenyls, Appl. Microbiol. Biotechnol., 2004, vol. 65, pp. 250–258.
Pieper, D.H., Aerobic Degradation of Polychlorinated Biphenyls, Appl. Microbiol. Biotechnol., 2005, vol. 67, pp. 170–191.
Bedard, D.L. and Quensen III, J.F., Microbial Reductive Dechlorination of Polychlorinated Biphenyls, Microbial Transformation and Degradation of Toxic Organic Chemicals, Young, Y. and Cerniglia, C.E., Eds., New York: Wiley-Liss, 1995, pp. 127–216.
Wiegel, J. and Wu, Q.Z., Microbial Reductive Dehalogenation of Polychlorinated Biphenyls, FEMS Microbiol. Letts., 2000, vol. 32, pp. 1–15.
Zanaroli, G., Perez-Jimenez, J.R., Young, L.Y., Marchetti, L., and Fava, F., Microbial Reductive Dechlorination of Weathered and Exogenous Co-Planar Polychlorinated Biphenyls (PCBs) in An Anaerobic Sediment of Venice Lagoon, Biodegradation, 2006, vol. 17, pp. 121–129.
Bedard, D.L., Bailey, J.J., Reiss, B.L., and Jerzak, G.V., Development and Characterization of Stable Sediment-Free Anaerobic Bacterial Enrichment Cultures That Dechlorinate Aroclor 1260, Appl. Environ. Microbiol., 2006, vol. 72, pp. 2460–2470.
Furukawa, K., Oxygenases and Dehalogenases: Molecular Approaches To Efficient Degradation of Chlorinated Environmental Pollutants, Boisci. Biotechnol. Biochem., 2006, vol. 70, pp. 2335–2348.
Ang, E.L., Zhao, H.M., and Obbard, J.P., Recent Advances in the Bioremediation of Persistent Organic Pollutants Via Biomolecular Engineering, Enz. Microbial Technol., 2005, vol. 37, pp. 487–496.
Kohler, H.-E.P., Kohler-Staub, D., and Focht, D.D., Cometabolism of Polychlorinated Biphenyls: Enhanced Transformation of Aroclor 1254 by Growing Bacterial Cells, Appl. Environ. Microbiol., 1988, vol. 54, pp. 1940–1945.
Rybkina, D.O., Plotnikova, E.G., Dorofeeva, L.V., Mironenko, Yu.L., and Demakov, V.A., A New Aerobic Gram-Positive Bacterium with a Unique Ability to Degrade ortho-and para-chlorinated Biphenyls, Mikrobiologiya, 2003, vol. 72, no. 6, pp. 759–765 [Microbiology (Engl. Transl.), vol. 72, no. 6, pp. 672–677].
Di Gioia, D., Bertin, L., Zanaroli, G., Marchetti, L., and Fava, F., Polichlorinated Biphenyl Degradation in Aquaous Wastes by Employing Continious Fixed-Bed Bioreactors, Process Biochem., 2006, vol. 41, pp. 935–940.
Brunner, W., Sutherland, F.H., and Focht, D.D., Enhanced Biodegradation of Polychlorinated Biphenyls in Soil by Analog Enrichment and Bacterial Inoculation, J. Environ. Qual., 1985, vol. 14, pp. 324–328.
Komancova, M., Jurcova, I., Kochankova, L., and Burkhard, J., Metabolic Pathways of Polychlorinated Biphenyls Degradation by Pseudomonas sp. 2, Chemosphere, 2003, vol. 50, pp. 537–543.
Keum, Y.S. and Li, Q.X., Fungal Laccase-Catalyzed Degradation of Hydroxy Polychlorinated Biphenyls, Chemosphere, 2004, vol. 56, pp. 23–30.
Kamei, I., Kogura, R., and Kondo, R., Metabolism of 4,4′-Dichlorobiphenyl by White-Rot Fungi Phanerochaete chrysosporium and Phanerochaete sp. MZ142, Appl. Microbiol. Biotech., 2006, vol. 72, pp. 566–575.
Kubatova, A., Erbanova, P., Eichlerova, I., Homolka, L., Nerud, F., and Sasek, V., PCB Congener Selective Biodegradation by the White Rot Fungus Pleurotus ostreatus in Contaminated Soil, Chemosphere, 2001, vol. 43, pp. 207–215.
Zharikov, G.A., Borovick, R.V., Kapranov, V.V., Kiselyova, N.I., Krainova, O.A., Dyadishcheva, V.P., Salanda, A.V., and Zharikov, M.G., Study of Contamination and Migration of Polychlorinated Biphenyls in the Environment. Bioremediation of Contaminated Soils and Assessment of Their Impact on the Serpukhov Population Health, Bioremediation of Soils Contaminated with Aromatic Compounds, Heipiper H.J., Ed., Springer, 2007, pp. 93–104.
Natarajan, M.R., Wu, W.M., Wang, H., Bhatnagar, L., and Jain, M.K., Dechlorination of Spiket PCBs in Lake Sediment by Anaerobic Microbial Granules, Water. Res., 1998, vol. 32, pp. 3013–3020.
Adriaens, P. and Grbic-Grlic, D., Cometabolic Transformation of Mono-and Dichlorobiphenyls and Chlorohydroxybiphenyls by Methanotrophic Groundwater Isolates, Environ. Sci. Technol., 1994, vol. 28, pp. 1325–1330.
Nollet, H., Van de Putte, I., Raskin, L., and Verstraete, W., Carbon/Electron Source Dependence of Polychlorinated Biphenyl Dechlorination Pathways for Anaerobic Granules, Chemosphere, 2005, vol. 58, pp. 299–310.
Tartakovsky, B., Michote, A., Cadieux, J.-A.C., Lau, P.C.K., Hawari, J., and Guiot, S.R., Degradation of Aroclor 1242 in a Single-Stage Coupled Anaerobic/Aerobic Bioreactor, Water Res., 2001, vol. 35, pp. 4323–4330.
Moza, P., Scheunert, I., Klein, W., and Korte, F., Studies with 2,4′5-Trichlorobiphenyl-14C and 2,2′,4,4′,5-Pentaclorobiphenyl-14C in Carrots, Sugar Beets, and Soil, J. Agr. Food Chem., 1979, vol. 27, pp. 1079–1124.
Focht, D.D. and Brunner, W., Kinetics of Biphenyl and Polychlorinated Biphenyl Metabolism in Soil, Appl. Environ. Microbiol., 1985, vol. 50, pp. 1058–1063.
Vasilyeva, G.K, Strijakova, E.R, and Shea, P.J, Use of Activated Carbon for Soil Bioremediation, Viable methods of soil and water pollution monitoring, protection and remediation, Twardowska, I. et al. Eds., Serial NATO Collection, Springer, 2006.
Bzdusek, P.A., Lu, J., and Christensen, E.R., PCB Congeners and Dechlorination in Sediments of Sheboygan River, Wisconsin, Determined by Matrix Factorization, Environ. Sci. Technol., 2006, vol. 40, pp. 120–129.
Cho, Y.C., Sokol, R.C., Frohnhoefer, R.C., and Rhee, G.Y., Reductive Dechlorination of Polychlorinated Biphenyls: Threshold Concentration and Dechlorination Kinetics of Individual Congeners in Aroclor 1248, Environ. Sci. Technol., 2003, pp. 5651–5656.
Zeeb, B.A., Amphlet, J.S., Rutter, A., and Reimer, K.J., Potential for Phytoremediation of Polychlorinated Biphenyl-(PCB)-Contaminated Soil, Int. J. Phytoremedia, 2006, vol. 8, pp. 199–221.
Sinkhonen, S. and Paasivirta, J., Degradation Half-Life Times of PCDDs, PCDFs and PCBs for Environmental Fate Modeling, Chemosphere, 2000, vol. 40, pp. 943–949.
Ayris, S. and Harrad, S., The Fate and Persistence of Polychlorinated Biphenyls in Soil, J. Environ. Monitor, 1999, vol. 1, pp. 395–401.
Fava, F., Bertin, L., Fedi, S., and Zannoni, D., Methyl-Beta-Cyclodextrin-Enhanced Solubilization and Aerobic Biodegradation of Polychlorinated Biphenyls in Two Aged-Contaminated Soils, Biotechnol. Bioeng., 2003, vol. 81, pp. 381–390.
Chang, B.V., Liu, W.G., and Yuan, S.Y., Microbial Dechlorination of Three PCBs Congeners in River Sediment, Chemosphere, 2001, vol. 45, pp. 849–856.
Kwon, O.S., Kim, Y.E., and Park, J.G., Effect of Moisture Content on Reductive Dechlorination of Polychlorinated Biphenyls and Population Dynamics of Dechlorinating Microorganisms, J. Microbiol., 2001, vol. 39, pp. 195–201.
Rysavy, J.P., Yan, T., and Novak, P.J., Enrichment of Anaerobic Polychlorinated Biphenyl Dechlorinators from Sediment with Iron as a Hydrogen Source, Water Res., 2005, vol. 39, pp. 569–578.
Alexander, M., Biodegradation and bioremediation, CA, USA: Academic Press, 1999.
Harkness, M.R., McDermott, J.B., Abramowicz, D.A., Salvo, J.J., Flanagan, W.P., Stephens, M.L., Mondello, F.J., May, R.J., Lobos, J.H., Carrol, K.M., Brennan, M.J., Bracco, A.A., Fish, K.M., Warner, G.L., Wilson, P.R., Dietrich, D.K., Lin, D.T., Morgan, C.B., and Gately, W.L., In Situ Stimulation of Aerobic PCB Biodegradation in Hudson River Sediments, Science, 1993, vol. 259, pp. 503–507.
Oh, E.T., Koh, S.C., Kim, E., Ahn, Y.H., and So, J.S., Plant Terpenes Enhance Survivability of Polychlorinated Biphenyl (PCB) Degrading Pseudomonas pseudoalcaligenes KF707 Labeled with gfp in Microcosms Contaminated with PCB, J. Appl. Microbiol. Biotechnol., 2003, vol. 13, pp. 463–468.
Singer, A.C., Gilbert, E.S., Luepromchai, E., and Crowley, D.E., Bioremediation of Polychlorinated Biphenyl-Contaminated Soil Using Carvone and Surfactant-Grown Bacteria, Appl. Microbiol. Biochem., 2000, vol. 54, pp. 838–843.
Mulligan, C.N., Yong, R.N., and Gibbs, B.F., Surfactant-Enhanced Remediation of Contaminated Soil: a Review, Eng. Geology, 2001, vol. 60, pp. 371–380.
Hudak, A.J. and Cassidy, D.P., Stimulating In-Soil Rhamnolipid Production in a Bioslurry Reactor by Limiting Nitrogen, Biotechnol. Bioeng., 2004, vol. 88, pp. 861–868.
Doick, K.J., Burauel, P., Jones, K.C., and Semple, K.T., Effect of Cyclodextrin and Transformer Oil Amendments on the Chemical Extractability of Aged [C-14]Polychlorinated Biphenyl and [C-14]Polycyclic Aromatic Hydrocarbon Residues in Soil, Environ. Toxicol. Chem., 2005, vol. 24, pp. 2138–2144.
Jonker, M.T.O. and Barendregt, A., Oil Is Sedimentary Supersorbent for Polychlorinated Biphenyls, Environ. Sci. Technol., 2006, vol. 40, pp. 3829–3835.
Sylvestre, M., Biphenyl/Chlorobiphenyls Catabolic Pathway of Comamonas testosteroni B-356: Prospect for Use in Bioremediation, Int. Biodeter. Biodegr., 1995, pp. 189–211.
Ahn, Y.B., Beaudette, L.A., Lee, H., and Trevors, J.T., Survival of a gfp-Labeled Polychlorinated Biphenyl Degrading Psychrotolerant Pseudomonas spp. in 4 and 22 Degrees C Soil Microcosms, Microbial. Ecology, 2001, vol. 42, pp. 614–623.
Sierra, I., Valera, J.L., Marina, M.L., and Laborda, F., Study of the Biodegradation Process of Polychlorinated Biphenyls in Liquid Medium and Soil by a New Isolated Aerobic Bacterium (Janibacter sp.), Chemosphere, 2003, vol. 53, pp. 609–618.
Di Toro, S., Zanaroli, G., and Fava, F., Intensification of the Aerobic Bioremediation of an Actual Site Soil Historically Contaminated by Polychlorinated Biphenyls (PCBs) Through Bioaugmentation with a Non Acclimated, Complex Source of Microorganisms, Microbial. Cell. Factories, 2006, vol. 6, pp. 1–10.
Maltseva, O.V., Tsoi, T.V., Quensen III, J.F., Fukuda, M., and Tiedje, J.M., Degradation of Anaerobic Reductive Dechlorination Products of Aroclor 1242 by Four Aerobic Bacteria, Biodegradation, 1999, vol. 10, pp. 363–371.
Smith, K.E., Schwab, A.P., and Banks, M.K., Phytoremediation of Polychlorinated Biphenyl (PCB)-Contaminated Sediment: A Greenhouse Feasibility Study, J. Environ. Quality, 2007, vol. 36, pp. 239–244.
Kuipers, B., Cullen, W.R., and Mohn, W.W., Reductive Dechlorination of Weathered Aroclor 1260 during Anaerobic Biotreatment of Arctic Soils, Can. J. Microbiol., 2003, vol. 49, pp. 9–14.
Rodrigues, J.L.M., Kachel, C.A., Aiello, M.R., Quensen III, J.F., Maltseva, O.V., Tsoi, T.V., and Tiedje, J.M., Degradation of Aroclor 1242 Dechlorination Products in Sediments by Burkholderia xenovorans LB400(Ohb) and Rhodococcus sp. Strain RHA1 (Fcb), Appl. Environ. Microbiol., 2006, vol. 72, pp. 2476–2482.
Tharakan, J., Tomlinson, D., Addagada, A., and Shafagati, A., Biotransformation of PCBs in Contaminated Sludge: Potential for Novel Biological Technologies, Eng. Life Sci., 2006, vol. 6, pp. 43–50.
Patureau, D. and Trably, E., Impact of Anaerobic and Aerobic Processes on PolychloroBiphenyl Removal in Contaminated Sewage Sludge, Biodegradation, 2006, vol. 17, pp. 9–17.
Shimura, M., Hayakawa, T., Kyotani, T., Ushiogi, T., and Kimbara, K., Bioremediation of Polychlorinated Biphenyl Contaminated Sludge and Ballast, Proc. Institution of Mech. Eng. Part F-J. Rail and Rapid Transit, 2003, vol. 217, pp. 285–290.
Ryslava, E., Krejcik, Z., Macek, T., Novakova, H., Denmerova, K., and Mackova, M., Study of PCB Degradation in Real Contaminated Soil, Fres. Environ. Bull, 2003, vol. 12, pp. 296–301.
Villacieros, M., Whelan, C., Mackova, M., Molgaard, J., Sanchez-Contreras, M., Lloret, J., de Carcer, D.A., Oruezabal, R.I., Bolanos, L., Macek, T., Karlson, U., Dowling, D.N., Martin, M., and Rivilla, R., Polychlorinated Biphenyl Rhizoremediation by Pseudomonas fluorescens F113 Derivatives, Using a Sinorhizobium meliloti Nod System To Drive bph Gene Expression, Appl. Environ. Microbiol., 2005, vol. 71, pp. 2687–2694.
Chen, Y.Q., Adam, A., Toure, O., and Dutta, S.K., Molecular Evidence of Genetic Modification of Sinorhizobium meliloti: Enhanced PCB Bioremediation, J. Ind. Microbiol. Biotechnol., 2005, vol. 32, pp. 561–566.
Manzano, M.A., Perales, J.A., Sales, D., and Quiroga, J.M., Enhancement of Aerobic Microbial Degradation of Polychlorinated Biphenyl in Soil Microcosms, Environ. Toxicol. Chem., 2003, vol. 22, pp. 699–705.
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Original Russian Text © G.K. Vasilyeva, E.R. Strijakova, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 6, pp. 725–741.
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Vasilyeva, G.K., Strijakova, E.R. Bioremediation of soils and sediments contaminated by polychlorinated biphenyls. Microbiology 76, 639–653 (2007). https://doi.org/10.1134/S002626170706001X
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DOI: https://doi.org/10.1134/S002626170706001X